Frequency and specificity of alloantibodies to HNA system antigens in donors of blood and blood components

Abstract

Aim. To study the rate of human neutrophil antigens (HNA) alloimmunization in donors of blood and blood components.

Methods. The study included blood samples of 1,127 donors who donated blood and its components in the Russian Scientific and Research Institute of Hematology and Transfusiology, Saint Petersburg, 635 male and 492 female. 36.18% of female donors were aged between 18 and 30 (n=178) and 63.82% were aged between 30 and 60 (n=314). The medical histories of all donors did not contain the records about the transfusion of blood components. Screening and identification of HNA alloantibodies were performed using the flow cytometric granulocyte immunofluorescence test with monoclonal antibodies CD16-PE, CD45-PC7, CD19-APC, Goat F(ab')2 Anti-Human IgG-FITC and the panel of donor neutrophils with detected HNA genotypes.

Results. The frequency of HNA alloantibodies in blood donors was 0.35% (n=4). No HNA alloantibodies were found in the male donors. Alloantibodies were detected only in female blood donors aged between 30 and 60. HNA antibody frequency in female donors was 0.81%. It were detected anti-HNA-1a, anti-HNA-3b and anti-HNA-4b antibodies of the IgG immunoglobulin class. In one donor, the specificity of antibodies could not be established. To establish whether detected antibodies in immunized donors were alloantibodies, HNA genotypes were determined.

Сonclusion. HNA alloantibodies occur with low frequency and are determined in 0.35% of blood donors. Alloantibodies were detected only in female donors and were directed against HNA-1a, HNA-3b and HNA-4b. The frequency of HNA antibodies in female donors was 0.81%. HNA genotyping confirmed that detected antibodies were alloantibodies.

Keywords

HNA alloantibodies / transfusion-related acute lung injury / screening of alloantibodies / flow cytometry / donors / genotyping

References

[1] Davoren A., Curtis B.R., Shulman I.A. et al. ­TRALI due to granulocyte-agglutinating human neutrophil antigen-3a (5b) alloantibodies in donor plasma: a report of 2 fatalities. Transfusion. 2003; 43: 641–645. DOI: 10.1046/j.1537-2995.2003.00374.x.

[2] Reil A., Keller-Stanislawski B., Bux J. et al. Specificities of leucocyte alloantibodies in transfusion-­related acute lung injury and results of leucocyte antibody screening of blood donors. Vox Sang. 2008; 95: 313–317. DOI: 10.1111/j.1423-0410.2008.01092.x.

[3] Chapman C., Stainsby D., Jones H. et al. Ten years of hemovigilance reports of transfusion-related acute lung injury in the United Kingdom and the impact of preferential use of male donor plasma. Transfusion. 2009; 49: 440–452. DOI: 10.1111/j.1537-2995.2008.01948.x.

[4] Heinzl M.W., Schonbacher M., Dauber E.-M. et al. Detection of granulocyte-reactive antibodies: a comparison of different methods. Vox Sang. 2015; 108: 287–293. DOI: 10.1111/vox.12227.

[5] Gottschall J.L., Triulzi D.J., Curtis B. et al. The frequency and specificity of human neutrophil antigen antibodies in a blood donor population. Transfusion. 2011; 51: 820–827. DOI: 10.1111/j.1537-2995.2010.02913.x.

[6] Xia W., Xu X., Chen D. et al. The prevalence of leucocyte alloantibodies in blood donors from South China. Transfusion Med. 2015; 25: 385–392. DOI: 10.1111/tme.12276.

[7] Khaydukov S.V., Baidun L.A., Zurochka A.V., Totolyan A.A. Standardized technology “study of the subpopulation composition of peripheral blood lymphocytes using flow cytofluorimeters-analyzers” (project). Medicinskaya immunologiya. 2012; 14 (3): 255–268. (In Russ.)

[8] Хайдуков С.В., Байдун Л.А., Зурочка А.В., Тотолян А.А. Стандартизованная технология «исследование субпопуляционного состава лимфоцитов периферической крови с применением проточных цитофлюориметров-анализаторов» (проект). Мед. иммунол. 2012; 14 (3): 255–268. DOI: 10.15789/1563-0625-2012-3-255-268.

[9] Krobinets I.I., Mineeva N.V., Bogdanova I.O. et al. The value of the human neutrophil antigens typing of blood donors for the activity of the blood service. Transfusiologiya. 2018; (4): 23–31. (In Russ.)

[10] Кробинец И.И., Минеева Н.В., Богданова И.О. и др. Значение типирования антигенов нейтрофилов у доноров для деятельности службы крови. Трансфузиология. 2018; (4): 23–31.

[11] Mraz G.A., Crighton G.L., Christie D.J. Antibodies to human neutrophil antigen HNA-4b implicated in a case of neonatal alloimmune neutropenia. Transfusion. 2016; 56: 1161–1165. DOI: 10.1111/trf.13463.

[12] Marın L., Torıo A., Muro M. et al. Alloimmune neonatal neutropenia and thrombocytopenia associa­ted with maternal anti HNA-1a, HPA-3b and HLA antibo­dies. Pediatr. Allergy Immunol. 2005; 16: 279–282. DOI: 10.1111/j.1399-3038.2005.00245.x.

[13] Bayat B., Tjahjono Y., Berghofer H. et al. Choline transporter-like protein-2: New von Willebrand factor-binding partner involved in antibody-mediated neutrophil activation and transfusion-related acute lung injury. Arterioscler. Thromb. Vasc. Biol. 2015; 35 (7): 1616–1622. DOI: 10.1161/ATVBAHA.115.305259.

RIGHTS & PERMISSIONS

Krobinets I.I., Mineeva N.V., Bogdanova I.O., Kudryavtsev I.V., Chechetkin A.V.