Peripheral blood erythrocytes morphology in ovarian cancer

Abstract

Aim. To assess the morphology of circulating red blood cells in patients with stage III ovarian cancer.

Methods. The object of the study was the peripheral blood erythrocytes of primary patients with ovarian cancer (n=25) who had stage III according to International Federation of Gynecology and Obstetrics. Patients were examined in the gynecological department of Ulyanovsk Regional Clinical Oncology Center. The control group included 25 somatically healthy women. Morphological studies were performed using light microscopy. The number of red blood cells with an unchanged and altered shape was expressed as a percentage. By the method of atomic force microscopy, the topology and rigidity of red blood cells was studied.

Results. A statistically significant decrease in the number of circulating blood erythrocytes was found in patients with ovarian cancer compared to somatically healthy women. At the same time, the number of discocytes is markedly reduced while the number of morphologically altered forms: echinocytes, stomatocytes, spherocytes and erythrocyte rigidity are increased.

Conclusion. With the appearance of altered forms of red blood cells and increase of the transformation index and erythrocyte rigidity in patients with stage III ovarian cancer, total number of red blood cells decreases in circulating blood compared to somatically healthy women.

Keywords

ovarian cancer / red blood cells / echinocytes / transformation index

References

[1] Vaupel P., Thews O., Hoeckel M. Treatment resistance of solid tumors. Med. Oncol. 2001; 18: 243–259. DOI: 10.1385/MO:18:4:243.

[2] Vaupel P., Harrison L. Tumor hypoxia: causa­tive factors, compensatory mechanisms, and cellular response. Oncologist. 2004; 9: 4–9. DOI: 10.1634/theoncologist.9-90005-4.

[3] Schito L., Rey S. Hypoxic pathobiology of breast cancer metastasis. Biochim. Biophys. Acta. 2017; 1868: 239–245. DOI: 10.1016/j.bbcan.2017.05.004.

[4] Wolff M., Kosyna F.K., Dunst J. et al. Impact of hypoxia inducible factors on estrogen receptor expression in breast cancer cells. Arch. Biochem. Biophys. 2017; 613: ­23–30. DOI: 10.1016/j.abb.2016.11.002.

[5] Siprov A.V., Solov'eva M.A. Morphofunctional state of erythrocytes of rats with carcinoma Walker-256 in combined use of docetaxel and ximedon. Byulleten' eksperimental'noy biologii i meditsiny. 2017; (7): 56–60. (In Russ.)

[6] Сипров А.В., Соловьёва М.А. Морфофункциональное состояние эритроцитов крыс с карциномой Walker-256 при сочетанном применении доцетаксела и ксимедона. Бюлл. эксперим. биол. и мед. 2017; (7): 56–60.

[7] Sladkova E.A., Skorkina M.Yu., Zabinyakov N.A. Мorphological and functional peculiarities of blood cells at tumor growth condition. Biomeditsina. 2013; (3): 63–67. (In Russ.)

[8] Сладкова Е.А., Скоркина М.Ю., Забиняков Н.А. Морфофункциональные особенности клеток крови в условиях опухолевого роста. Биомедицина. 2013; (3): 63–67.

[9] Kozinets G.I., Pogorelov V.M., Shmarov D.A. et al. Kletki krovi. Sovremennye tekhnologii ih analiza. (Blood cells. Modern technologies of their analysis.) Moscow: Triada-Farm. 2002; 200 p. (In Russ.)

[10] Козинец Г.И., Погорелов В.М., Шмаров Д.А. и др. Клетки крови. Современные технологии их анализа. М.: Триада-Фарм. 2002; 200 p.

[11] Henderson R.M., Oberleithner H. Pushing, pulling, dragging, and vibrating renal epithelia by using atomic forse microscopy. AJP Renal physiol. 2000; 278 (5): 689–701. DOI: 10.1152/ajprenal.2000.278.5.F689.

[12] Dauletpaeva Zh.O., Demina E.V., Pan'shina S.S. State of peripheral red blood parameters in the presence of lung tumors. Sci. Time. 2016; (5): 159–160. (In Russ.)

[13] Даулетпаева Ж.О., Дёмина Е.В., Паньшина С.С. Состояние параметров красной периферической крови при наличии опухолей лёгкого. Sci. Time. 2016; (5): 159–160.

[14] Pumpur A.S. Role of the assessing the parameters of a complete blood count, biochemical blood assay and hemistasiograms in patients with colorectal cancer. Koloproktologiya. 2017; (3S): 64. (In Russ.)

[15] Пумпур А.С. Роль оценки показателей общего анализа крови, биохимического анализа крови и гемостазиограммы пациентов с колоректальным раком. Колопроктология. 2017; (3S): 64.

[16] Kozinets G.I., Vysotskiy V.V., Pogorelov V.M. Krov' i infekciya. (Blood and infection.) Moskow: Triada-farm. 2001; 182 p. (In Russ.)

[17] Козинец Г.И., Высоцкий В.В., Погорелов В.М. Кровь и инфекция. М.: Триада-фарм. 2001; 182 с.

[18] Novitskiy V.V., Stepovaya E.A., Gol'dberg V.E. Eritrotsity i zlokachestvennye novoobrazovanija. (Erythrocytes and malignant neoplasms.) Tomsk. 2000; 286 p. (In Russ.)

[19] Новицкий В.В., Степовая Е.А., Гольдберг В.Е. Эритроциты и злокачественные новообразования. Томск. 2000; 286 с.

[20] Pleskova S.N. Atomno-silovaya mikroskopiya v biologicheskih i medicinskih issledovaniyah. (Atomic force microscopy in biological and medical studies.) Dolgoprudnyy. 2011; 184 p. (In Russ.)

[21] Плескова С.Н. Атомно-силовая микроскопия в биологических и медицинских исследованиях. Долгопрудный. 2011; 184 с.

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Fedotova A.Y., Gening T.P., Abakumova T.V., Dolgova D.R.