Comparative measurement of concentration of lithium, circulating immune complexes and complement activity in blood and lymph in experimental anaphylactic shock and Arthus phenomenon

Abstract

Aim. Comparative measurement of concentration of lithium, circulating immune complexes and complement activity in animals’ blood and lymph in experimental anaphylactic shock and Arthus phenomenon.

Methods. Experiments were conducted on 27 chinchilla rabbits in double series. The studied values of lithium, concentration of circulating immune complexes and complement activity in intact animals were used as controls.

Results. On day 7 of sensibilisation before anaphylactic shock the titer of complement components in the blood decreased by 1.4 times (p <0.001) compared to parameters of intact animals, at the stage of anaphylactic shock sharp decrease of its concentration to undetermined values was observed (p <0.001). The blood concentration of circulating immune complexes increased by 4.4 times, and at the stage of anaphylactic shock (day 21) its increase by 7.1 times was observed (p <0.001). Lithium concentration decreased to 0.03 mmol/L at the stage of anaphylactic shock (p <0.001). In Arthus phenomenon at the stage of sensibilisation (day 5) the blood complement titer decreased by 1.1 times (p <0.001), during resolution period - by 10.1 times (p <0.001). The blood concentration of circulating immune complexes increased by 4.5 times (p <0.001) at the stage of sensibilisation (day 5), and at the stage of Arthus phenomenon - by 11.8 times compared to intact animals (p <0.001).

Conclusion. The concentration of circulating immune complexes increases in anaphylactic and immunocomplex reactions, besides, more significantly in Arthus phenomenon (23 r.u. in anaphylactic shock versus 38.1 r.u. in Arthus phenomenon); lithium concentration in anaphylactic shock decreases by 5.2 times which is indicative of inhibitory action of this microelement in allergic reactions.

Keywords

lithium / circulating immune complexes / complement / anaphylactic shock / Arthus phenomenon

References

[1] Podkolzin A.A., Dontsov V.I. Faktory maloy intensivnosti v bioaktivnosti i immunokorrektsii. (Factors of low intensity in the bioactivity and immunecorrection.) Moscow: Meditsina. 1985; 280 р. (In Russ.)

[2] Подколзин А.А., Донцов В.И. Факторы малой интенсивности в биоактивности и иммунокоррекции. М.: Медицина. 1985; 280 с.

[3] Dontsov VI., Ismailov T.A. Application of the lithium in the treatment of bronchial asthma. Patologicheskaya fiziologiya i eksperimental’naya terapiya. 1984; 5: 65–66. (In Russ.)

[4] Донцов В.И., Исмаилов Т.А. Применение микроэлемента лития при лечении бронхиальной астмы. Патол. физиол. и эксперим. терап. 1984; 5: 65-66.

[5] Gushchin I.S. IgE-mediated hypersensitivity as a response to barrier tissue dysfunction. Immunologiya. 2015; 36 (1): 45–52. (In Russ.)

[6] Гущин И.С. IgE-опосредованная гиперчувствительность как ответ на нарушение барьерной функции тканей. Иммунология. 2015; 36 (1): 45-52.

[7] Shatokhin M.N., Konoplya A.I., Teodorovich O.V., Karaulov A.V. Cytokine status and the complement system in chronic prostatitis. Pharmacological correction of violations. Immunologiya. 2011; 32 (1): 321–324. (In Russ.)

[8] Шатохин М.Н., Конопля А.И., Теодорович О.В., Караулов А.В. Цитокиновый статус и система комплемента при хроническом простатите. Фармакологическая коррекция нарушений. Иммунология. 2011; 32 (1): 321-324.

[9] Ettinger R., Karnell J.L., Henault J. et all. Pathogenic mechanisms of IgE-mediated imflamattion in self-destruktive autoimmune responses. Autoimmunity. 2017, 50 (1): 25–36. DOI: 10.1080/08916934.2017.1280670.

[10] Ettinger R., Karnell J.L., Henault J. et all. Pathogenic mechanisms of IgE-mediated imflamattion in self-destruktive autoimmune responses. Autoimmunity. 2017, 50 (1): 25-36. DOI: 10.1080/08916934.2017.1280670.

[11] Swierzko A., Sedzynski M., Kirikae T. et al. Role of the complement-lectin pathway in anaphylactoid reaction induced with lipopolysaccharide in mice. Eur. J. Immunol. 2003; 33: 2842–2852. DOI: 10.1002/eji.200323949.

[12] Swierzko A., Sedzynski M., Kirikae T. et al. Role of the complement-lectin pathway in anaphylactoid reaction induced with lipopolysaccharide in mice. Eur. J. Immunol. 2003; 33: 2842-2852. DOI: 10.1002/eji.200323949.

[13] Barkagan Z.S., Sukhanova Q.A., Buevich E.I. Anaphilactic shock. Konsilium. 2000; (6): 6–9. (In Russ.)

[14] Баркаган З.С., Суханова Г.А., Буевич Е.И. Анафилактический шок. Консилиум. 2000; (6): 6-9.

[15] Kornienko A.A., Kulikovskiy N.N.., Sorokatyy A.E. Catheterization of the thoracic duct in the experiment. Aktual’nye voprosy topograficheskoy anatomii i operativnoy khirurgii.. 1977; 1: 22–26. (In Russ.)

[16] Корниенко А.А., Куликовский Н.Н., Сорокатый А.Е. Катетеризация грудного протока в эксперименте. Актуал. вопр. топографич. анатомии и опер. хирургии. 1977; 1: 22-26.

[17] Lehmann W.D., Bahr U., Schulten H.R. Determination of lithium in microlitre amounts of human body fluids at therapeutic and normal levels by stable isotope dilution and field desorption mass spectrometry. Biomed. Mass. Spectrom. 1978; 9 (5): 536–539. DOI: 10.1002/bms.1200050906.

[18] Lehmann W.D., Bahr U., Schulten H.R. Determination of lithium in microlitre amounts of human body fluids at therapeutic and normal levels by stable isotope dilution and field desorption mass spectrometry. Biomed. Mass. Spectrom. 1978; 9 (5): 536-539. DOI: 10.1002/bms.1200050906.

[19] Grynevich Yu.A., Alferov A.N. Determination of immune complexes in the blood of cancer patients. Laboratornoe delo. 1981; (8): 493–495. (In Russ.)

[20] Гриневич Ю.А., Алфёров А.Н. Определение иммунных комплексов в крови онкологических больных. Лабораторн. дело. 1981; (8): 493-495.

[21] Reznikovа L.S. Komplement i ego znachenie v immunologicheskikh reaktsiyakh. (Complement and its importance in immunological reactions.) Moscow: Meditsina. 1967; 272 р. (In Russ.)

[22] Резникова Л.С. Комплемент и его значение в иммунологических реакциях. М.: Медицина. 1967; 272 с.

[23] Aliev S.D., Akhundov I.A. Changes in immune parameters in anaphylactic reactions and their correction by microelements. Scientifical and pedagogical news of Odlar Yurdu University, Baku. 2005; (14): 86–89. (In Russ.)

[24] Алиев С.Д., Ахундов И.А. Изменения иммунных показателей при анафилактических реакциях и их коррекция микроэлементами. Odlar Yurdu Universitetinin elmi və pedaqoji xəbərləri. 2005; (14): 86-89.

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Alieva T.R.