Morphological characteristics of experimental ischemic wound healing after application of autoand hetero-fibroblasts and dermal equivalent

Abstract

Aim. To study morphological structure, collagen synthesis and angiogenesis in biopsy samples of newly formed epidermis and derm on day 19 of their repair in experimental ischemic wound after application of auto- and hetero-fibroblasts as well as after transplantation of dermal equivalent with hetero-fibroblasts.

Methods. The study was performed on 28 mature C57/B1 white mice younger than 1 year. Around and into the bed of surgical skin wound in the scapular region, 0.4 ml of suspension of fibroblasts and 1.33 million cells of dermal equivalent were injected. Biopsy sample was embedded in paraffin and was stained with hematoxylin and eosin and by Weigert-Van Gieson.

Results. Most importantly, regenerative histogenesis is affected by introduction of auto-fibroblasts, when the greatest thickness of the epidermis is registered and angiogenesis and collagen formation are most active. At the same time, the most differentiated epidermis is observed after transplantation into the wound of dermal equivalent with hetero-bifroblasts.

Conclusion. The beneficial effect of dermal equivalent with hetero-bifroblasts differs from the effect of auto-fibroblast suspension by only a few percent: epidermal thickness by 4.29%, the area of collagen fibers by 2.66%, the area of blood vessels by 4.04%, that makes such differences unreliable.

Keywords

regenerative histogenesis / dermal equivalent / fibroblast / skin / angiogenesis

References

[1] Govrin G. New method for treating hard-to-heal wounds: clinical experience with charged polystyrene microsphers. Wounds. 2010; 6 (4): 52–61.

[2] Govrin G. New method for treating hard-to-heal wounds: clinical experience with charged polystyrene microsphers. Wounds. 2010; 6 (4): 52-61.

[3] Danilov R.K. Ranevoy protsess: gistogeneticheskie osnovy. (Wound process: histogenetic basis.) Saint Petersburg: VMedA. 2008; 380 p. (In Russ.)

[4] Данилов Р.К. Раневой процесс: гистогенетические основы. СПб.: ВМедА. 2008; 380 с.

[5] Khrupkin V.I., Zubritskiy V.F., Ivashkin A.N. et al. Dermatoplastika ranevykh defektov. (Dermatoplasty of wound defects.) Moscow: GEOTAR-Media. 2009; 102 p. (In Russ.)

[6] Хрупкин В.И., Зубрицкий В.Ф., Ивашкин А.Н. и др. Дерматопластика раневых дефектов. М: ГЭОТАР-Медиа. 2009; 102 с.

[7] Vinnik Yu.S., Salmina A.B., Drobushevskaya A.I. et al. Cellular technologies and tissue engineering in the treatment of long-term healing wounds. Vestnik eksperimental’noy i klinicheskoy khirurgii. 2011; 4 (2): 392–397. (In Russ.)

[8] Винник Ю.С., Салмина А.Б., Дробушевская А.И. и др. Клеточные технологии и тканевая инженерия в лечении длительно не заживающих ран. Вестн. эксперим. и клин. хир. 2011; 4 (2): 392-397.

[9] Shapovalova E.Yu., Boyko T.A., Baranovskiy Yu.G. et al. Morphological characteristics of the healing of an ischemic experimental wound on the 12th day after application of auto- and hetero-fibroblasts and dermal equivalent. Mezhdunarodnyy nauchno-issledovatel’skiy zhurnal. 2017; (8-3): 51–55. (In Russ.)

[10] Шаповалова Е.Ю., Бойко Т.А., Барановский Ю.Г. и др. Морфологическая характеристика заживления ишемизированной экспериментальной раны на 12 сутки после применения ауто- и гетерофибробластов и дермального эквивалента. Международ. науч.-исслед. ж. 2017; (8-3): 51-55.

[11] Porter S. The role of the fibroblast in wound contraction and healing. Wounds. 2007; 3 (2): 45–48.

[12] Porter S. The role of the fibroblast in wound contraction and healing. Wounds. 2007; 3 (2): 45-48.

[13] Zorin V.L., Zorina A.I., Petrakova O.S., Cherkasov V.R. Dermal fibroblasts for the treatment of skin defects. Geny & Kletki. 2009; 4 (4): 26–40. (In Russ.)

[14] Зорин В.Л., Зорина А.И., Петракова О.С., Черкасов В.Р. Дермальные фибробласты для лечения дефектов кожи. Гены & Клетки. 2009; 4 (4): 26-40.

[15] Meleshina A.V., Bystrova A.S., Rogovaya O.S. et al. Tissue-engineered skin constructs and application of stem cells for creation of skin equivalents (review). Sovremennye tekhnologii v meditsine. 2017; 9 (1): 198–218. (In Russ.) DOI: 10.17691/stm2017.9.1.24.

[16] Мелешина А.В., Быстрова А.С., Роговая О.С. и др. Тканеинженерные конструкты кожи и использование стволовых клеток для создания кожных эквивалентов (обзор). Соврем. технол. в мед. 2017; 9 (1): 198-218. DOI: 10.17691/stm2017.9.1.24.

[17] Baranovskiy Yu.G., Il’chenko F.N., Shapovalova E.Yu. Method for modeling trophic ulcers in laboratory mice in the experimental model. Vestnik neotlozhnoy i vosstanovitel’noyk hirurgii. 2016; 1 (2): 258–260. (In Russ.)

[18] Барановский Ю.Г., Ильченко Ф.Н., Шаповалова Е.Ю. Способ моделирования трофической язвы у лабораторных мышей в опытной модели. Вестн. неотложной и восстановительной хир. 2016; 1 (2): 258-260.

[19] Andreev D.Yu., Abramova N.V., Blinova M.I., Pinaev G.P. Effectiveness of cutaneous plastics and dermal equivalent in the treatment of extensive ulcers of the tibia of mixed origin. Vestnik khirurgii im. I.I. Grekova. 2013; 172 (1): 104–107. (In Russ.)

[20] Андреев Д.Ю., Абрамова Н.В., Блинова М.И., Пинаев Г.П. Эффективность кожной пластики и дермального эквивалента в лечении обширных язв голени смешанного генеза. Вестн. хир. им. И.И. Грекова. 2013; 172 (1): 104-107.

RIGHTS & PERMISSIONS

Shapovalova E.Y., Boyko T.A., Baranovskiy Y.G., Lugin I.A.