Changes of intestinal microbiota in patients with HIV

Abstract

Aim. To assess the changes in intestinal microbiota in patients with HIV. Methods. The cultures of stool samples of 317 patients with different stages of HIV [13 (4.1%) patients - with the acute infection stage (2A, 2B, 2C), 217 (68.5%) - with the stage III, 87 (27.4%) - with the stages IVA-B according to classification by Pokrovsky V.I., 2001] were examined by plating on the growth medium and further microbiologic examination. Results. Changes in intestinal microbiota were found in 94% of the patients. The decrease in the obligatory microorganisms quantity, especially in bifidobacteria (lower than reference ranges in 70.3% of patients) was the most frequent finding. Bacterial overgrowth of conditionally pathogenic biota (mainly S. aureus and Candida) was registered in 57.1% of patients. The intestinal microbiota composition did not depend on the clinical stage of HIV infection and presence of opportunistic infections, with only CD-4 count associated with the degree of intestinal microbiota changes. Conclusion. Intestinal microbiota changes are typical for patients with HIV infection at all stages of the disease regardless of opportunistic infections presence. Low CD-4 count is associated with higher severity of intestinal microbiota changes.

Keywords

HIV infection / intestinal microbiota changes / systemic inflammatory response syndrome

References

[1] Хасанова Г.Р., Биккинина О.И., Анохин В.А. и др. Кишечный эндотоксин как вероятный индуктор системного воспалительного ответа при ВИЧ-инфекции // Практ. мед. - 2012. - №1. - C. 52-55.

[2] Anukam K.C., Osazuwa E.O., Osadolor H.B. et al. Yogurt containing probiotic Lactobacillus rhamnosus GR-1 and L. reuteri RC-14 helps resolve moderate diarrhea and increases CD4 count in HIV/AIDS patients // J. Clin. Gastroenterol. - 2008. - Vol. 42. - P. 239-243.

[3] Brenchley J.M., Price D.A., Schacker T.W. et al. Microbial translocation is a cause of systemic immune activation in chronic HIV infection // Nat. Med. - 2006. - Vol. 12. - P. 1365-1371.

[4] Brenchley J.M., Douek D.C. HIV infection and the gastrointestinal immune system // Mucosal Immunol. - 2008. - Vol. 1. - P. 23-30.

[5] Gaboriau-Routhiau V., Rakotobe S., Lecuyer E. et al. The key role of segmented filamentous bacteria in the coordinated maturation of gut helper T cell responses // Immunity. - 2009. - Vol. 31. - P. 677-689.

[6] Grantham-McGregor S., Cheung Y.B., Cueto S. et al. Developmental potential in the first 5 years for children in developing countries // Lancet. - 2007. - Vol. 369. - P. 60-70.

[7] Kwon H.K., Lee C.G., So J.S. et al. Generation of regulatory dendritic cells and CD4+ Foxp3+ T cells by probiotics administration suppresses immune disorders // Proc. Natl. Acad. Sci. USA. - 2010. - Vol. 107. - P. 2159-2164.

[8] Lin Y.P., Thibodeaux C.H., Pena J.A. et al. Probiotic Lactobacillus reuteri suppress proinflammatory cytokines via c-Jun // Inflamm. Bowel Dis. - 2008. - Vol. 14. - P. 1068-1083.

[9] Nazli A., Chan O., Dobson-Belaire W.N. et al. Exposure to HIV-1 directly impairs mucosаl epithelial barrier integrity allowing microbial translocation // PLoS Pathog. - 2010. - Vol. 6, N 4. - ID-e1000852.

[10] Sharpstone D., Neild P., Crane R. et al. Small intestinal transit, absorption, and permeability in patients with AIDS with and without diarrhea // Gut. - 1999. - Vol. 45. - P. 70-76.

[11] Trois L., Cardoso E.M., Miura E. Use of probiotics in HIV-infected children: a randomized double-blind controlled study // J. Trop. Pediatr. - 2008. - Vol. 54. - P. 19-24.

[12] Willing B.P., Van Kessel A.G. Enterocyte proliferation and apoptosisin the caudal small intestine is influenced by the composition of colonizing commensal bacteria in the neonatal gnotobiotic pig // J. Anim. Sci. - 2007. - Vol. 85. - P. 3256-3266.

RIGHTS & PERMISSIONS

Khasanova G.R., Anokhin V.A., Bikkinina O.I., Shakhbazova E.N., Kotlyar E.Y., Nagimova F.I.