Genetic markers of predisposition to oral lichen planus recurrence development

Abstract

Aim. To search for genetic markers of oral lichen planus development and recurrence risk based on the study of polymorphic variants of tumor necrosis factor α (TNFα rs1800630, rs1800629, rs361525) and interleukin-18 (IL-18 rs187238) genes in patients from the Volga-Ural region of Russia.

Methods. Standard methods of molecular genetic analysis were used in the study. Deoxyribonucleic acid was isolated from peripheral blood lymphocytes by deproteinization with phenol and chloroform. Genotyping of studied polymorphic loci was performed by real time polymerase chain reaction using TaqMan-competing probes. In order to identify the association of polymorphic loci in different models (additive, dominant, recessive, overdominant) the method of logistic regression was used.

Results.. Statistical analysis using logistic regression revealed that polymorphic locus rs187238 in IL-18 gene is associated with the risk of lichen planus development in a recessive model (p=0.042). In addition, the association of a polymorphic locus rs187238 in IL-18 gene with the recurrence risk was described: rs187238*C/C genotype is a genetic marker of increased risk of oral lichen planus recurrence (p=0.01). Analysis of the association of polymorphic loci rs1800630, rs1800629, rs361525 in tumor necrosis factor α gene with the disease severity and the recurrence risk did not reveal any statistically significant results.

Conclusion. The study results confirm the cytokine genes contribution to the oral lichen planus development and disease reccurrence.

Keywords

oral lichen planus / cytokines / polymorphic variants of genes / genetic risk marker / disease recurrence

References

[1] Анисимова И.В., Недосеко В.Б., Ломиашвили Л.М. Клиника, диагностика и лечение заболеваний слизистой оболочки рта и губ. М.: Медкнига: Стоматология. 2008; 117-130 c.

[2] Al-Mohaya M.A., Al-Harthi F., Arfin M., Al-Asmari A. TNF-α, TNF-β and IL-10 gene polymorphism and association with oral lichen planus risk in Saudi patients. J. Appl. Oral Sci. 2015; 23 (3): 295-301. http://dx.doi.org/10.1590/1678-775720150075

[3] Bai J., Zhang Y., Lin M. et al. Interleukin-18 gene polymorphisms and haplotypes in patients with oral lichen planus: a study in an ethnic Chinese cohort. Tissue Antigens. 2007; 70: 390-397. http://dx.doi.org/10.1111/j.1399-0039.2007.00922.x

[4] Boraschi D., Dinarello C.A. IL-18 in autoimmunity: review. Eur. Cytokine Netw. 2006; 17: 224-252.

[5] Carrozzo M., Dametto E., Fasano M.E. et al. Cytokine gene polymorphisms in hepatitis C virus-related oral lichen planus. Exp. Dermatol. 2007; 16: 730-736. http://dx.doi.org/10.1111/j.1600-0625.2007.00577.x

[6] Chauhan I., Beena V.T., Srinivas L. et al. Association of cytokine gene polymorphisms with oral lichen planus in Malayalam-speaking ethnicity from South India (Kerala). J. Interferon Cytokine Res. 2013; 33: 420-427. http://dx.doi.org/10.1089/jir.2012.0115

[7] Hsu H.J., Yang Y.H., Shieh T.Y. et al. Role of cytokine gene (interferon-γ, transforming growth factor-β1, tumor necrosis factor-α, interleukin-6, and interleukin-10) polymorphisms in the risk of oral precancerous lesions in Taiwanese. Kaohsiung J. Med. Sci. 2014; 30 (11): 551-558. http://dx.doi.org/10.1016/j.kjms.2014.09.003

[8] Jin X., Wang J., Zhu L. et al. Association between -308 G/A polymorphism in TNF-a gene and lichen planus: a meta-analysis. J. Dermatol. Sci. 2012; 68: 127-134. http://dx.doi.org/10.1016/j.jdermsci.2012.09.003

[9] Kimkong I., Hirankarn N., Nakkuntod J., Kitkumthorn N. Tumour necrosis factor-alpha gene polymorphisms and susceptibility to oral lichen planus. Oral Dis. 2011; 17: 206-209. http://dx.doi.org/10.1111/j.1601-0825.2010.01722.x

[10] Lu R., Zhang J., Sun W. et al. Inflammation-related cytokines in oral lichen planus: an overview. J. Oral Pathol. Med. 2015; 44: 1-14. http://dx.doi.org/10.1111/jop.12142

[11] Preshaw P.M., Taylor J.J. How has research into cytokine interactions and their role in driving immune responses impacted our understanding of periodontitis? J. Clin. Periodontol. 2011; 38 (11): 60-84. http://dx.doi.org/10.1111/j.1600-051X.2010.01671.x

[12] Sugerman P.B., Savage N.W., Walsh L.J. et al. The pathogenesis of oral lichen planus. Crit. Rev. Oral Biol. Med. 2002; 13: 350-365. http://dx.doi.org/10.1177/154411130201300405

[13] Thongprasom K., Dhanuthai K., Sarideechaigul W. et al. Expression of TNF-alpha in oral lichen planus treated with fluocinolone acetonide 0.1%. J. Oral Pathol. Med. 2006; 35: 161-166. http://dx.doi.org/10.1111/j.1600-0714.2006.00392.x

[14] Zhang Y., Liu W., Zhang S. et al. Salivary and serum interleukin-18 in patients with oral lichen planus: a study in an ethnic Chinese population. Inflammation. 2012; 35: 399-404. http://dx.doi.org/10.1007/s10753-011-9327-3

[15] Zhou G., Xia K., Du G.F. et al. Activation of nuclear factor kappa B correlates with tumor necrosis factor-alpha in oral lichen planus: a clinicopathologic study in atrophic-erosive and reticular form. J. Oral Pathol. Med. 2009; 38: 559-564. http://dx.doi.org/10.1111/j.1600-0714.2009.00779.x

RIGHTS & PERMISSIONS

Akmalova G.M., Chuykin S.V., Ron G.I., Chernysheva N.D., Galimova E.S., Gilyazova I.R., Khusnutdinova E.K.