Effect of recombinant glycodelin on spleen structure in case of allogeneic bone marrow cells introduction

Abstract

Objective. To study the effect of recombinant glycodelin (Mybiosource, Germany) on the morphofunctional state of the spleen in case of transplantation of the allogeneic red bone marrow cells to Wistar rats in dynamics of in vivo experiment. From the point of view of immunology, pregnancy is a physiologically conditioned state of the tolerance of mother’s immune system to genetically foreign embryo. Glycodelin is a protein associated with pregnancy; it has an immunosuppressive effect and is perspective for medicine.

Materials and methods. The morphological picture of the organ was assessed; the following immunohistochemical indicators were studied: monoclonal antibodies to 1) CD68 – for identification of macrophages, membrane staining; 2) Ki-67 – for cells divided with mitosis and being in different phases of cellular cycle; 3) determination of macrophage colony-stimulating factor (M-CSF).

Results. When studying the histological slices of the spleen, it was shown that glycodelin against the background of allogeneic transplantation of the bone marrow contributes to the activation of immune system cells in the spleen, stimulates the proliferation of immune cells (Ki-67) and their differentiation that was manifested by an increase in the number of plasmacytes. By the end of the study, macrophage content is essentially reduced; eosinophil infiltration is verified that is an indirect positive sign of reaction to the transplant. Against the background of the bone marrow cells allotransplantation, there was observed an increase in M-CSF level in animals on the day 21^st from the onset the experiment compared with the group of intact animals. Introduction of glycodelin against the background of BM cells allotransplantation caused the cancellation of this effect.

Conclusions. Thus, the action of glycodelin qualitatively determined the function of the spleen in direction of the development of a tolerant immune response to allogenate and excluded the development of severe post-transplantation complications.

Keywords

allogeneic transplant / glycodelin / red bone marrow / spleen / proliferation / macrophages

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