Contribution of polymorphism of genes of aldosterone synthetase CYP11B2 (C-344T), adrenergic receptors type 1 ADRB1 (Gly389Arg) and type 2 ADRB2 (Arg16Gly) to formation of different obesity phenotypes

Abstract

Objective. To study the relationship of polymorphism of the genes of aldosterone synthetase CYP11B2 (C-344T), adrenergic receptor type 1 ADRB1 (Gly389Arg) and type 2 ADRB2 (Arg16Gly) with the risk of obesity.

Materials and methods. The study involved 170 patients; the mean age was 45.4 ± 7.3 years. The patients were divided into 2 groups: group 1 – obese patients with metabolic disorders in combination with 1-2 degree hypertension – complicated obesity (n = 90); group 2 – 50 participants with obesity without metabolic disorders (metabolically healthy obesity). The control group consisted of 100 healthy respondents. All the surveyed persons underwent anthropometry and general clinical studies according to the recommendation for examination of patients with obesity and arterial hypertension. Total DNA was isolated from venous whole blood samples by RT-PCR using the DNA-Sorb-B kit.  

Results. It was found that in the group of complicated obesity, the carriage of the TT genotype of the CYP11B2 gene polymorphism (C-344T) and the CG genotype of the Gly389Arg polymorphism of the ADRB1 gene prevailed in comparison with the group of metabolically healthy obesity and healthy respondents. The predominance of carriage of the GG genotype of the ADRB2 gene polymorphism (Arg16Gly) was revealed in the groups of metabolically healthy obesity and complicated obesity in comparison with the healthy group. An association of the polymorphic position of the ADRB1 (Gly389Arg) and ADRB2 (Arg16Gly) genes with the level of low-density lipoprotein cholesterol, triglycerides, the level of systolic blood pressure and diastolic blood pressure, and the level of uric acid was established. The relationship between the polymorphism of the CYP11B2 (C-344T) promoter region and the level of glomerular filtration rate and total cholesterol was determined.

Conclusions. Analysis of ADRB1 (Gly389Arg), ADRB2 (Arg16Gly), and CYP11B2 (C-344T) gene polymorphism variants can be used as an additional marker to assess the risk of developing obesity, arterial hypertension, and metabolic disorders.

Keywords

obesity / arterial hypertension / CYP11B2 (C-344T) / ADRB1 (Gly389Arg) / ADRB2 (Arg16Gly) gene polymorphisms

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RIGHTS & PERMISSIONS

Shulkina S.G., Larina O.S., Alikin S.V., Zhelobov V.G., Smirnova E.N., Antipova A.A., Kolomeets N.Y., Kobernik M.Y.