Influence of polymorphism of the gene GCLC on antioxidant defense status in healthy children of Omsk region

Abstract

Aim. To study the status of antioxidant defense in healthy children, living in Omsk Region, depending on polymorphism of the gene GCLC.

Materials and methods. There were examined 50 healthy children aged 3 to 17 years, including 54 % of boys. Polymorphism of the gene GCLC 129С/C, 129C/Т, 129T/T and reduced (GSH) and oxidized (GSSG) glutathione concentrations were determined. Statistical analysis was implemented using the programs «Statistica 6.0 and DoctorStat1.9.

Results. Polymorphism of the gene GCLC was represented by the genotypes 129C/C (43 children, 86 %) and 129C/T (7 children, 14 %); GSH concentration was within 6.0 [4.4; 7.0] mcmol/l, GSSG concentration – 3.0 [2.3; 3.5] mcmol/l, GSH:GSSG ratio was 2:1. Weak direct correlation between the child age and GSH (r = 0.31; р = 0.028) and GSSG (r = 0.31; р = 0.031) content was established; no gender differences were revealed. Among the subjects with the genotype 129C/T, the blood plasma GSH (р = 0.017) and GSSG (р = 0.016) concentrations decreased by 30 %.

Conclusions. Glutathione concentration in children depends on polymorphism of the gene GCLC.

Keywords

Children / glutathione / gene polymorphism

References

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RIGHTS & PERMISSIONS

Pavlinova E.B., Kirshina I.A., Kurmasheva E.I., Vlasenko N.Y., Mingairova A.G., Savchenko O.A., Indutnyi A.V., Novikov D.G.