PHENOTYPICAL AND MOLECULAR-GENETIC MONITORING OF NEISSERIA GONORRHOEAE, CIRCULATING IN WESTERN URAL

M A Rumyantseva; A V Kolomoitsev; T I Karpunina; V D Elkin

doi:10.17816/pmj34460-66

Perm Medical Journal ›› 2017, Vol. 34 ›› Issue (4) :60 -66. DOI: 10.17816/pmj34460-66

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PHENOTYPICAL AND MOLECULAR-GENETIC MONITORING OF NEISSERIA GONORRHOEAE, CIRCULATING IN WESTERN URAL

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Abstract

Aim. To study the biological peculiarities of N.gonorrhoeae strains, isolated from patients of Perm Krai. Materials and methods. Gonococcal infection sickness rate for 2010-2015 in Perm Krai was analyzed using intensive indices. Sixty clinical N.gonorrhoeae strains were used in this work. Primary identification of cultures was carried out at Regional Venereologic Dispensary using bacterioscopic and bacteriological methods with standard surgical procedures. To confirm the species belonging of isolated cultures, PCR method in real-time mode was applied. Results. An approximate value of the average annual increase was 5,85 %. It was shown that use of molecular-genetic analysis essentially adds to phenotypical monitoring of N.gonorrhoeae that permits to assess genetic variety and mechanisms of resistance to antibacterial agents of the strains, circulating throughout the administrative territory. Conclusions. The first experience of such studies showed genetic variety of N.gonorrhoeae , circulating in Perm Krai, and revealed a number of peculiarities of gene prevalence, determining their sensibility/resistance to antimicrobial drugs.

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N. gonorrhoeae / Monitoring / N.gonorrhoeae / phenotypical and molecular-genetic methods of analysis

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M A Rumyantseva, A V Kolomoitsev, T I Karpunina, V D Elkin. PHENOTYPICAL AND MOLECULAR-GENETIC MONITORING OF NEISSERIA GONORRHOEAE, CIRCULATING IN WESTERN URAL. Perm Medical Journal, 2017, 34(4): 60-66 DOI:10.17816/pmj34460-66

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