ANALYSIS OF MORPHOLOGICAL CHANGES IN INTACT AND STAPHYLOCOCCUS AUREUS № 6 INFECTED MICE WHEN IMMUNIZED WITH PROTEIN-CONTAINING ANTIGENS

Abstract

Aim. To study the morphological changes in the organs of intact mice and Staphylococcus aureus № 6 infected animals, introduced different S.aureus -secreted protein-containing antigens (PCA) and their protective activity. Materials and methods. 180 male mice of BALB/c line were studied. Mice were doubly immunized subcutaneously in the volume of 0,1 ml with different doses of “original” PCA (0,75; 1,8; 3,0 and 12 mkg), PCA I and PCA II (1,2mkg). Morphological changes in mice organs were investigated after introducing different doses of PCA without animals infection and with preliminary immunization on the model of generalized process development, when infected in the retroorbital sinus with the sublethal dose of S.aureus № 6 equal to 0,1 LD50. To conduct morphological studies, spleen, lungs and right kidney of experimental animals were taken. Paraffin sections of the organs including serial kidney sections (with the interval of 200 mcm) were stained with hematoxylin and eosin. When generalized staphylococcal infection in experimental animals was formed, there was developed and used a “renal” model with the offered indices of abscess process formation and parallel analysis of inoculation results. Results. It was established that complex study of the influence of S.aureus- secreted PCA on the parameters of adaptive immunity, including the study of antigenic and protective activity, morphological changes in the organs, use of “renal” model with the developed indices of abscess process formation and parallel analysis of inoculation results, is informative and adequate. Conclusions. The secreted protein-containing antigens - “original” and PCA II - are the promising preparations to be studied and used for the development of antistaphylococcal vaccines.

Keywords

Staphylococcus aureus № 6 / Protein-containing antigens / Staphylococcus aureus № 6 / morphological changes / “renal model”

References

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RIGHTS & PERMISSIONS

Astashkina E.A., Lebedinskaya O.V., Gruber I.M.