CYTOMEGALOVIRUS DETECTION FREQUENCY IN INFANTS BORN OWING TO USE OF AUXILIARY REPRODUCTIVE TECHNOLOGIES

A V Permyakova; I I Lvova; S E Makhmudova

doi:10.17816/pmj34251-55

Perm Medical Journal ›› 2017, Vol. 34 ›› Issue (2) :51 -55. DOI: 10.17816/pmj34251-55

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CYTOMEGALOVIRUS DETECTION FREQUENCY IN INFANTS BORN OWING TO USE OF AUXILIARY REPRODUCTIVE TECHNOLOGIES

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Abstract

Aim. To determine the frequency of detecting cytomegaloviral infection (CMVI) in infants born as a result of extracorporal fertilization (ECF). Materials and methods. Medical documentation and examination of 46 infants under 1 year, who were born by means of ECF, were analyzed. To detect CMV DNA and determine its quantitative content, PCR in a real-time mode (test-system “AmpliSens® SMV-screen/monitor-FL” (Russia)) was used. Saliva and urine served as the study material. Results. CMV DNA, determined in the saliva and urine, indicated the following: in 78,3 % (36/46) of infants, no viral DNA was revealed in any of media. In 21,7 % (10/46) of infants, salivary CMV DNA was detected, in 17,4 % (8/46) - urinary. Viral load median of CMV DNA in the saliva was 5,5l g copies of DNA/ml (confidence interval, CI - 4,9; 6,1), in the urine - 4,6 lg copies of DNA/ml (CI - 4,0; 5,2) with no reliable difference for the studied media. Finally, the share of CMV-positive infants, born by means of ECF within the first year of life was 21,7 %. Conclusions. Infants, born owing to ECF, in 65,2 % of cases are premature; CMVI frequency, verified by PCR-positive salivary and urinary tests among them is 21,0 %.

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Auxiliary reproductive technologies / cytomegaloviral infection / infants / screening

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A V Permyakova, I I Lvova, S E Makhmudova. CYTOMEGALOVIRUS DETECTION FREQUENCY IN INFANTS BORN OWING TO USE OF AUXILIARY REPRODUCTIVE TECHNOLOGIES. Perm Medical Journal, 2017, 34(2): 51-55 DOI:10.17816/pmj34251-55

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