封面图片
2022年, 第16卷 第7期
Cover illustration
Targeting at the conflict between growth and production, a dual temperature control system is developed for yeast engineering based on the expression and activity control of the transcriptional activator Gal4, which acts as a switch to regulate the direction of carbon flux. Temperature change serves as an input signal to trigger the expression of the Gal4 mutant under a heat-shock promoter, and meanwhile to activate it due to its cold-sensitive feature. By changing the culture temperature from 30 °C to 37 °C, both the expression level and activity of the Gal4 mutant are increased, which re-directs the carbon flux from cell growth to product synthesis. In this way, the cellular burden caused by isoprene biosynthesis via a mitochondria-compartmented pathway could be relieved, leading to reconstruction of balance between cell growth and isoprene production. (Jiaxi Lin, Zhen Yao, Xiaomei Lyu, Lidan Ye, Hongwei Yu, pp. 1079–1089)[展开] ...
ISSN 2095-0179 (Print)
ISSN 2095-0187 (Online)
CN 11-5981/TQ
Postal Subscription Code 80-969 Formerly Known as Frontiers of Chemical Science and Engineering in China 2018 Impact Factor: 2.809