Identification of key genes and signaling pathways based on transcriptomic studies of aerobic and resistance training interventions in sarcopenia in SAMP8 mice

Abstract

We examined the effects of resistance and aerobic exercise on the gene expression and biometabolic processes of aging skeletal muscle in senescence-accelerated mouse/prone 8 mice, a model of sarcopenia, and compared them with senescence-accelerated mouse/resistant 1 mice acting as controls. We found that exercise improved muscle strength, endurance, fiber size, also modulated genes and pathways related to synaptic transmission, potassium transport, JAK-STAT signaling, and PI3K-Akt signaling. Our results suggested that BDNF, JAK2, RhoC, Myh6, Stat5a, Tnnc1, and other genes may mediate the beneficial effects of exercise on sarcopenia through these pathways.

Keywords

Resistance training / Aerobic training / Sarcopenia / Transcriptome

Submission statement

The manuscript has not been published and is not under consideration for publication elsewhere. All authors have read and agree with the manuscript content, and the manuscript will not be submitted elsewhere for review and publication.

Ethical approval statement for animal use

Male SAMR1 and SAMP8 mice (7 months of age) were purchased from Peking University School of Medicine (Department of Laboratory Animal Science), license number: SCXK (Beijing) 2016-0010. Animal feeding and training were carried out in the Animal Experiment Center of Chengdu Sport University (license number: Ethics Committee of Chengdu Sport University 2021 No. 39). The laboratory room temperature was controlled at 20 °C-25 ​°C, and a good ventilation environment and ambient humidity were guaranteed for 12 ​h. Lights on and lights off simulated day and night alternation. The mice had free access to standard SPF-grade full-price solid nutrient feed and filtered warm water. The litter tray and animal laboratory were cleaned daily.

Authors’ contributions

DH: conceptualization, methodology, validation, writing—original draft preparation, and project administration. LL and GX: software, formal analysis, investigation, resources, supervision, and funding acquisition. LL, HY, YB, and QB: writing—review and editing. All authors have read and agreed to the published version of the manuscript.

Funding

Funding was received from the National Natural Science Foundation of China (NSFC) (NSFC Grant No. 81904318), Sichuan Science and Technology Program (22ZYZYTS0046), Key Laboratory of Sports Medicine of Sichuan Province, Institute of Sports Medicine and Health, Chengdu Sport University (No. 2021-A030), and the National Key Research and Development Program of China (No. 2018YFF0300904), Sichuan Province central government guides local science and technology development project (2022ZYD0062), Sichuan Province science and technology innovation and entrepreneurship seedling project (MZGC20230033).

CRediT authorship contribution statement

Lunyu Li: Writing - original draft, Supervision, Software, Resources, Methodology, Funding acquisition, Formal analysis, Data curation. Xiaotian Guan: Writing - original draft, Supervision, Software, Resources, Investigation, Funding acquisition, Formal analysis. Ying Huang: Visualization, Validation, Software, Formal analysis. Bo Qu: Visualization, Software, Methodology. Binyu Yao: Supervision, Software, Formal analysis. Haili Ding: Writing - review & editing, Project administration, Conceptualization.

Conflict of interest

There no conflicts of interest.

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The authors thank all the laboratory members (Qingsong Liu, Junjie Zhu, Zeting Fu, Yan Zhang, and Yicheng Wang) for their help and technical support. We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.