Construction of circular RNA-based competing endogenous RNA network in colon cancer

  • Zhengyu Gu 1 ,
  • Weiyi Wu 2 ,
  • Jinnan Lu 3 ,
  • Jun Shen , 4
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  • 1. Department of Gastroenterology, Nanjing Red Cross Hospital, Nanjing, China
  • 2. Department of Pathophysiology, Key Laboratory of Cardiovascular Disease and Molecular Intervention, Nanjing Medical University, Nanjing, China
  • 3. Department of Gastroenterology, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, Jiangsu, People's Republic of China
  • 4. Department of Synthetic Internal Medicine, Nanjing Red Cross Hospital, Nanjing, China
99528161@qq.com

Received date: 15 Aug 2023

Revised date: 22 Feb 2024

Accepted date: 22 Feb 2024

Copyright

2024 2024 The Authors. Precision Medical Sciences published by John Wiley & Sons Australia, Ltd on behalf of Nanjing Medical University Affiliated Cancer Hospital & Jiangsu Cancer Hospital.

Abstract

Circular RNAs (CircRNAs) are now under discussion as novel promising biomarkers for patients with various tumors. Our study aimed to identify circRNAs related to the progression of colon cancer and to further investigate their roles in the development of colon cancer base on The Cancer Genome Atlas database. The competing endogenous RNA (ceRNA) network was constructed based on 4 circRNAs, 14 miRNAs, and 229 mRNAs. Meanwhile, three hub genes were identified via the protein protein interaction networks (PPI) network. It should be noted that only RUNX1 targeted by circ-0084615 and mir-330-3p was confirmed as a survival-related gene. Further analyses indicated the possible function of RUNX1 in colon progression, such as angiogenesis, epithelial–mesenchymal transition, hedgehog signaling and so on. Further investigations were conducted into the correlation between RUNX1 expression profiles and immune cells. Finally, we validated the expression of the circ-0084615/mir-330-3p/RUNX1 axis in clinical specimens. In conclusion, we constructed ceRNA network and revealed that the circ-0084615/mir-330-3p/RUNX1 axis serves as a critical role in colon cancer.

Cite this article

Zhengyu Gu , Weiyi Wu , Jinnan Lu , Jun Shen . Construction of circular RNA-based competing endogenous RNA network in colon cancer[J]. Precision Medical Sciences, 2024 , 13(1) : 52 -62 . DOI: 10.1002/prm2.12127

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