Construction of circular RNA-based competing endogenous RNA network in colon cancer

Abstract

Circular RNAs (CircRNAs) are now under discussion as novel promising biomarkers for patients with various tumors. Our study aimed to identify circRNAs related to the progression of colon cancer and to further investigate their roles in the development of colon cancer base on The Cancer Genome Atlas database. The competing endogenous RNA (ceRNA) network was constructed based on 4 circRNAs, 14 miRNAs, and 229 mRNAs. Meanwhile, three hub genes were identified via the protein protein interaction networks (PPI) network. It should be noted that only RUNX1 targeted by circ-0084615 and mir-330-3p was confirmed as a survival-related gene. Further analyses indicated the possible function of RUNX1 in colon progression, such as angiogenesis, epithelial–mesenchymal transition, hedgehog signaling and so on. Further investigations were conducted into the correlation between RUNX1 expression profiles and immune cells. Finally, we validated the expression of the circ-0084615/mir-330-3p/RUNX1 axis in clinical specimens. In conclusion, we constructed ceRNA network and revealed that the circ-0084615/mir-330-3p/RUNX1 axis serves as a critical role in colon cancer.

Key words： ceRNA; circRNAs; colon cancer; RUNX1; tumor progression

References

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