Distinguish on the viability of human umbilical cord mesenchymal stem cells using delayed luminescence

Abstract

In this paper, we report the discrimination of the viability of human umbilical cord mesenchymal stem cells (hUC-MSCs) with photo-induced delayed luminescence (DL). We measure the DL decay kinetics of hUC-MSCs using an ultraweak luminescence detection system, and find the significant difference in the weight distributions of the decay rate for hUC-MSCs with high and low viabilities. Spectral discrimination of hUC-MSCs with high and low viabilities is thus carried out by comparing the DL kinetics parameters, including the initial intensity, the peak decay rate and the peak weight value. Our results show that the novel optical method for the viability diagnosis of hUC-MSCs has a promising prospect.

Keywords

High Viability / Decay Rate Constant / Stem Cell Review / Peak Weight / Umbilical Cord Mesenchymal Stem Cell

References

[1] Bianco P, Cao X, Frenette P S, Mao J J, Robey P G, Simmons P J, Wang C-Y. Nature Medicine. 2013, 19: 35

[2] Ranganath S H, Levy O, Inamdar M S, Karp J M. Cell Stem Cell. 2012, 10: 244

[3] Wang S, Qu X, Zhao R C. Journal of Hematology and Oncology. 2012, 5: 19

[4] Lee J W, Fang X, Krasnodembskaya A, Howard J P, Matthay M A. Stem Cells. 2011, 29: 913

[5] Williams A R, Hare J M. Circulation Research. 2011, 109: 923

[6] Motaln H, Schichor C, Lah T T. Cancer. 2010, 116: 2519

[7] Lu L L, Liu Y J, Yang S G, Zhao Q J, Wang X, Gong W, Han Z B, Xu Z S, Lu Y X, Liu D, Chen Z Z, Han Z C. Haematologica. 2006, 91: 1017

[8] Baksh D, Yao R, Tuan R S. Stem Cells. 2007, 25: 1384

[9] Liu Y, Mu R, Wang S, Long L, Liu X, Li R, Sun J, Guo J, Zhang X, Guo J, Yu P, Liu C, Liu X, Huang Z, Wang D, Li H, Gu Z, Liu B, Li Z. Arthritis Research & Therapy. 2010, 12: R210

[10] Fan C-G, Zhang Q-J, Zhou J-R. Stem Cell Reviews and Reports. 2011, 7: 195

[11] Fang T-C, Pang C-Y, Chiu S-C, Ding D-C, Tsai R-K. PLoS ONE. 2012, 7: e46504

[12] Bongso A, Fong C-Y. Stem Cell Reviews and Reports. 2013, 9: 226

[13] Popp F-A, Yan Y. Physics Letters A. 2002, 293: 93

[14] Musumeci F, Applegate L A, Privitera G, Scordino A, Tudisco S, Niggli H J. Journal of Photochemistry and Photobiology B: Biology. 2005, 79: 93

[15] Musumeci F, Privitera G, Scordino A, Tudisco S, Presti C L, Applegate L A, Niggli H J. Applied Physics Letters. 2005, 86: 153902

[16] Lanzanò L, Scordino A, Privitera S, Tudisco S, Musumeci F. European Biophysics Journal. 2007, 36: 823

[17] Kim H-W, Sim S-B, Kim C-K, Kim J, Choi C, You H, Soh K-S. Cancer Letters. 2005, 229: 283

[18] Kim J, Choi C, Lim J, You H, Sim S-B, Yom Y-K, Kim E-H, Soh K-S. Journal of Alternative & Complementary Medicine. 2005, 11: 879

[19] Chen P, Zhang L, Zhang F, Liu J-T, Bai H, Tang G-Q, Lin L. Biomedical Optics Express. 2012, 3: 1787

[20] Bókkon I, Vimal R, Wang C, Dai J, Salari V, Grass F, Antal I. Journal of Photochemistry and Photobiology B: Biology. 2011, 103: 192

[21] Lenger K, Bajpai R P, Spielmann M. Identification of Unknown Homeopathic Remedies by Delayed Luminescence. Cell Biochemistry and Biophysics. 20131

[22] Bai H, Chen P, Fang H, Lin L, Tang G Q, Mu G G, Gong W, Liu Z P, Wu H, Zhao H, Han Z C. Laser Physics Letters. 2011, 8: 78

[23] Scordino A, Triglia A, Musumeci F. Journal of Photochemistry and Photobiology B: Biology. 2000, 56: 181