PDF
(4118KB)
Abstract
Apple polyphenols are widely present in apple pulp, peel, kernel, and leaves. They are natural bioactive substances with a variety of health functions. This review describes the antioxidant functions of anthocyanins, quercetin, phlorizin, chlorogenic acid, and epicatechin in apple polyphenols through the regulation of signal pathways and transcription factors, and the inhibition or induction of enzymes. Massive food applications of apple polyphenols are summarized, including excellent color protection of fruits and vegetables, meat preservation, preparation of composite packaging films as active ingredients, enhancement of nutritional value as functional ingredients, and improvement of food taste and physical stability. This review would provide a reference for the exploration of apple polyphenol resources and its development in the food field.
Keywords
polyphenols
/
antioxidant
/
apple
/
functional food
Cite this article
Download citation ▾
Qiuxin Lu, Xiaoya Pan, Liangjun Wu, Caihong Shi, Xiangrong Zhang.
Antioxidant mechanisms and applications of apple polyphenols in food: A review.
Journal of Polyphenols, 2025, 7(2): 47-58 DOI:
| [1] |
He Qiang. Polyphenols in fruits and vegetables and their functional properties[J]. Journal of Xihua University (Natural Science Edition), 2019, 38(4): 37-44.
|
| [2] |
Huang S, Li H, Wang L, et al. Research progress of antioxidant properties of apple polyphenols[J]. Food Research and Development, 2014, 35(24): 159-162.
|
| [3] |
Francini A, Romeo S, Cifelli M, et al. 1H NMR and PCA-based analysis revealed variety dependent changes in phenolic contents of apple fruit after drying[J]. Food Chem, 2017, 221: 1206-1213.
|
| [4] |
Kalinowska M, Bielawska A, Lewandowska-Siwkiewicz H, et al. Apples: Content of phenolic compounds vs. variety, part of apple and cultivation model, extraction of phenolic compounds, biological properties[J]. Plant Physiol Biochem, 2014, 84: 169-188.
|
| [5] |
Pojer E, Mattivi F, Johnson D, et al. The case for anthocyanin consumption to promote human health: A review[J]. Compr Rev Food Sci Food Saf, 2013, 12(5): 483-508.
|
| [6] |
Zhao CL, Chen ZJ, Bai XS, et al. Structure-activity relationships of anthocyanidin glycosylation[J]. Mol Divers, 2014, 18(3): 687-700.
|
| [7] |
Li H, Zhang C, Deng Z, et al. Antioxidant activity of delphinidin and pelargonidin: Theory and practice[J]. J Food Biochem, 2022, 46(8): e14192.
|
| [8] |
Kähkönen MP, Heinonen M. Antioxidant activity of anthocyanins and their aglycons[J]. J Agric Food Chem, 2003, 51(3): 628-633.
|
| [9] |
Ali HM, Almagribi W, Al-Rashidi MN. Antiradical and reductant activities of anthocyanidins and anthocyanins, structure-activity relationship and synthesis[J]. Food Chem, 2016, 194: 1275-1282.
|
| [10] |
Tena N, Martín J, Asuero AG. State of the art of anthocyanins: Antioxidant activity, sources, bioavailability, and therapeutic effect in human health[J]. Antioxidants (Basel), 2020, 9(5): 451.
|
| [11] |
Schlesier K, Harwat M, Böhm V, et al. Assessment of antioxidant activity by using different in vitro methods[J]. Free Radic Res, 2002, 36(2): 177-187.
|
| [12] |
Enaru B, Drețcanu G, Pop TD, et al. Anthocyanins: Factors affecting their stability and degradation[J]. Antioxidants (Basel), 2021, 10(12): 1967.
|
| [13] |
Dudek A, Spiegel M, Strugała-Danak P, et al. Analytical and theoretical studies of antioxidant properties of chosen anthocyanins; A structure-dependent relationships[J]. Int J Mol Sci, 2022, 23(10): 5432.
|
| [14] |
Zaplatic E, Bule M, Shah SZA, et al. Molecular mechanisms underlying protective role of quercetin in attenuating Alzheimer’s disease[J]. Life Sci, 2019, 224: 109-119.
|
| [15] |
Min Z, Yangchun L, Yuquan W, et al. Quercetin inhibition of myocardial fibrosis through regulating MAPK signaling pathway via ROS[J]. Pak J Pharm Sci, 2019, 32(3 Special): 1355-1359.
|
| [16] |
Valko M, Jomova K, Rhodes CJ, et al. Redox- and non- redox-metal-induced formation of free radicals and their role in human disease[J]. Arch Toxicol, 2016, 90(1): 1-37.
|
| [17] |
Cherrak SA, Mokhtari-Soulimane N, Berroukeche F, et al. In vitro antioxidant versus metal ion chelating properties of flavonoids: A structure-activity investigation[J]. PLoS One, 2016, 11(10): e0165575.
|
| [18] |
Kattoor AJ, Kanuri SH, Mehta JL. Role of Ox-LDL and LOX-1 in atherogenesis[J]. Curr Med Chem, 2019, 26(9): 1693-1700.
|
| [19] |
Rehman K, Akash MSH. Mechanism of generation of oxidative stress and pathophysiology of type 2 diabetes mellitus: How are they interlinked?[J]. J Cell Biochem, 2017, 118(11): 3577-3585.
|
| [20] |
Oyedemi SO, Nwaogu G, Chukwuma CI, et al. Quercetin modulates hyperglycemia by improving the pancreatic antioxidant status and enzymes activities linked with glucose metabolism in type 2 diabetes model of rats: In silico studies of molecular interaction of quercetin with hexokinase and catalase[J]. J Food Biochem, 2020, 44(2): e13127.
|
| [21] |
Ehrenkranz JR, Lewis NG, Kahn CR, et al. Phlorizin: A review[J]. Diabetes Metab Res Rev, 2005, 21(1): 31-38.
|
| [22] |
Liu Y, Liu Y, Guo Y, et al. Phlorizin exerts potent effects against aging induced by D-galactose in mice and PC12 cells[J]. Food Funct, 2021, 12(5): 2148-2160.
|
| [23] |
Bhakkiyalakshmi E, Sireesh D, Rajaguru P, et al. The emerging role of redox-sensitive Nrf2-Keap1 pathway in diabetes[J]. Pharmacol Res, 2015, 91: 104-114.
|
| [24] |
Park S, Park SK. Anti-oxidant and anti-aging effects of phlorizin are mediated by DAF-16-induced stress response and autophagy in caenorhabditis elegans[J]. Antioxidants (Basel), 2022, 11(10): 1996.
|
| [25] |
Santana-Gálvez J, Cisneros-Zevallos L, Jacobo-Velázquez DA. Chlorogenic acid: Recent advances on its dual role as a food additive and a nutraceutical against metabolic syndrome[J]. Molecules, 2017, 22(3): 358.
|
| [26] |
Lee TK, Kang IJ, Kim B, et al. Experimental pretreatment with chlorogenic acid prevents transient ischemia- induced cognitive decline and neuronal damage in the hippocampus through anti-oxidative and anti-inflammatory effects[J]. Molecules, 2020, 25(16): 3578.
|
| [27] |
Hayakawa S, Ohishi T, Miyoshi N, et al. Anti-cancer effects of green tea epigallocatchin-3-gallate and coffee chlorogenic acid[J]. Molecules, 2020, 25(19): 4553.
|
| [28] |
Pimpley V, Patil S, Srinivasan K, et al. The chemistry of chlorogenic acid from green coffee and its role in attenuation of obesity and diabetes[J]. Prep Biochem Biotechnol, 2020, 50(10): 969-978.
|
| [29] |
Ong KW, Hsu A, Tan BK. Anti-diabetic and anti-lipidemic effects of chlorogenic acid are mediated by ampk activation[J]. Biochem Pharmacol, 2013, 85(9): 1341-1351.
|
| [30] |
Salimi R, Naderi R, Shirpoor A. Involvement of miR- 27a/smurf1/ TNF-α and mitochondrial apoptotic pathway in apoptosis induced by cerebral ischemia-reperfusion injury in rats: The protective effect of chlorogenic acid[J]. Neuroscience Letters, 2023, 817: 137529.
|
| [31] |
FEAK M, Yao WY, Ding YM, et al. Chlorogenic acid suppresses mitochondrial apoptotic effectors Bax/Bak to counteract Nod‐like receptor pyrin domain 3 (NLRP3) inflammasome in thiram exposed chondrocytes[J]. Phytomedicine, 2022, 95: 153865.
|
| [32] |
Han D, Gu X, Gao J, et al. Chlorogenic acid promotes the Nrf2/HO-1 anti-oxidative pathway by activating p21(Waf1/Cip1) to resist dexamethasone-induced apoptosis in osteoblastic cells[J]. Free Radic Biol Med, 2019, 137: 1-12.
|
| [33] |
Wang Q, Liu T, Koci M, et al. Chlorogenic acid alleviated AFB1-induced hepatotoxicity by regulating mitochondrial function, activating Nrf2/HO-1, and inhibiting noncanonical NF-κB signaling pathway[J]. Antioxidants (Basel), 2023, 12(12): 2027.
|
| [34] |
Wang D, Hou J, Wan J, et al. Dietary chlorogenic acid ameliorates oxidative stress and improves endothelial function in diabetic mice via Nrf2 activation[J]. J Int Med Res, 2021, 49(1): 300060520985363.
|
| [35] |
Huang J, Xie M, He L, et al. Chlorogenic acid: A review on its mechanisms of anti-inflammation, disease treatment, and related delivery systems[J]. Front Pharmacol, 2023, 14: 1218015.
|
| [36] |
Gao F, Fu K, Li H, et al. Chlorogenic acid ameliorates mice clinical endometritis by activating Keap1/Nrf2 and inhibiting NFκB signalling pathway[J]. J Pharm Pharmacol, 2021, 73(6): 785-795.
|
| [37] |
Liang N, Kitts DD. Chlorogenic acid (CGA) isomers alleviate interleukin 8 (IL-8) production in Caco-2 cells by decreasing phosphorylation of p38 and increasing cell integrity[J]. Int J Mol Sci, 2018, 19(12): 3873.
|
| [38] |
Ouyang H, Du A, Zhou L, et al. Chlorogenic acid improves diabetic retinopathy by alleviating blood-retinal- barrier dysfunction via inducing Nrf2 activation[J]. Phytother Res, 2022, 36(3): 1386-1401.
|
| [39] |
Zheng Y, Li L, Chen B, et al. Chlorogenic acid exerts neuroprotective effect against hypoxia-ischemia brain injury in neonatal rats by activating Sirt1 to regulate the Nrf2-NF-κB signaling pathway[J]. Cell Commun Signal, 2022, 20(1): 84.
|
| [40] |
Liu D, Wang H, Zhang Y, et al. Protective effects of chlorogenic acid on cerebral ischemia/reperfusion injury rats by regulating oxidative stress-related Nrf2 pathway[J]. Drug Des Devel Ther, 2020, 14: 51-60.
|
| [41] |
Huang T, Che Q, Chen X, et al. Apple polyphenols improve intestinal antioxidant capacity and barrier function by activating the Nrf2/Keap1 signaling pathway in a pig model[J]. J Agric Food Chem, 2022, 70(24): 7576-7585.
|
| [42] |
Qu Z, Liu A, Li P, et al. Advances in physiological functions and mechanisms of (-)-epicatechin[J]. Crit Rev Food Sci Nutr, 2021, 61(2): 211-233.
|
| [43] |
Qing Y, Xiang X, Li S, et al. Integrated evaluation the antioxidant activity of epicatechin from cell dynamics[J]. Biotechnol Prog, 2023, 39(3): e3328.
|
| [44] |
Aron PM, Kennedy JA. Flavan-3-ols: Nature, occurrence and biological activity[J]. Mol Nutr Food Res, 2008, 52(1): 79-104.
|
| [45] |
Martín M, Fernández-Millán E, Ramos S, et al. Cocoa flavonoid epicatechin protects pancreatic beta cell viability and function against oxidative stress[J]. Mol Nutr Food Res, 2014, 58(3): 447-56.
|
| [46] |
González-Manzano S, González-Paramás AM, Delgado L, et al. Oxidative status of stressed Caenorhabditis elegans treated with epicatechin[J]. J Agric Food Chem, 2012, 60(36): 8911-8916.
|
| [47] |
Surco-Laos F, Dueñas M, González-Manzano S, et al. Influence of catechins and their methylated metabolites on lifespan and resistance to oxidative and thermal stress of Caenorhabditis elegans and epicatechin uptake[J]. Food Research International, 2012, 46(2): 514-521.
|
| [48] |
Martín M A, Ramos S, Mateos R, et al. Protection of human HepG2 cells against oxidative stress by the flavonoid epicatechin[J]. Phytother Res, 2010, 24(4): 503-509.
|
| [49] |
Chang CF, Cho S, Wang J. (-)-Epicatechin protects hemorrhagic brain via synergistic Nrf2 pathways[J]. Ann Clin Transl Neurol, 2014, 1(4): 258-271.
|
| [50] |
Shah ZA, Li RC, Ahmad AS, et al. The flavanol (-)-epicatechin prevents stroke damage through the Nrf2/ HO1 pathway[J]. J Cereb Blood Flow Metab, 2010, 30(12): 1951-1961.
|
| [51] |
Prince PD, Fraga CG, Galleano M. (-)-Epicatechin administration protects kidneys against modifications induced by short-term l-NAME treatment in rats[J]. Food Funct, 2020, 11(1): 318-327.
|
| [52] |
Zhang H, Deng A, Zhang Z, et al. The protective effect of epicatechin on experimental ulcerative colitis in mice is mediated by increasing antioxidation and by the inhibition of NF-κB pathway[J]. Pharmacol Rep, 2016, 68(3): 514-520.
|
| [53] |
Prince PD, Lanzi CR, Toblli JE, et al. Dietary (-)-epicatechin mitigates oxidative stress, NO metabolism alterations, and inflammation in renal cortex from fructose-fed rats[J]. Free Radic Biol Med, 2016, 90: 35-46.
|
| [54] |
Ma J, Peng X, Ng KM, et al. Impact of phloretin and phloridzin on the formation of Maillard reaction products in aqueous models composed of glucose and L-lysine or its derivatives[J]. Food Funct, 2012, 3(2): 178-186.
|
| [55] |
Ran J, Su Y, Wang P, et al. Effect of Lactobacillus acidophilus fermentation on bioaccessibility: The relationship between biotransformation and antioxidant activity of apple polyphenols based on metabolomics[J]. LWT, 2023, 190: 115360.
|
| [56] |
Yi JH, Dong XL, Zhu ZB. et al. Study on the inhibition mechanism of browning inhibitors on apple polyphenol oxidase[J]. Food & Machinery, 2015, 31(4): 122-125.
|
| [57] |
Wong-Paz JE, Muñiz-Márquez DB, Aguilar CN, et al. Enzymatic synthesis, purification and in vitro antioxidant capacity of polyphenolic oxidation products from apple juice[J]. LWT-Food Science and Technology, 2015, 64(2): 1091-1098.
|
| [58] |
Le Guernevé C, Sanoner P, Drilleau JF, et al. New compounds obtained by enzymatic oxidation of phloridzin[J]. Tetrahedron Letters, 2004, 45(35): 6673-6677.
|
| [59] |
Zhang S, Chou S, Cui H, et al. Effects of phloretin on rheological proper ties, antioxidant activity and microstructure of low ester pectin under acidic conditions[J]. Food Science, 2020, 41(2): 43-49.
|
| [60] |
Sun JX, Zhang XW, Chen LF, et al. Study on the antioxidant performance of apple polyphenols[J]. Food Research and Development, 2005(2): 149-150.
|
| [61] |
Lee MK, Hwang YH, Ryu H, et al. Galla rhois water extract inhibits enzymatic browning in apple juice partly by binding to and inactivating polyphenol oxidase[J]. Food Chemistry, 2022, 383: 132277.
|
| [62] |
Marrufo-Hernández NA, Nájera H, González Chávez F, et al. Polyphenol oxidase inactivation from apple juice by Al-based metal-organic frameworks: New anti-browning strategy in fruits and vegetables[J]. Food Chemistry, 2024, 439: 138178.
|
| [63] |
Du S, Zhang Z, Che L, et al. Study on the preservation effect of apple polyphenol and chitosan on pork[J]. Gansu Animal Husbandry and Veterinary Medicine, 2021, 51(8): 31-34+41.
|
| [64] |
Li Y, Liu W, Zhang S, et al. Effect of colorants on color and flavor of traditional chinese cured meat products[J]. Food Science, 2017, 38(19): 68-74.
|
| [65] |
Zhong Y, Liu Y, Xing L, et al. Improving the quality of frozen lamb by microencapsulated apple polyphenols: Effects on cathepsin activity, texture, and protein oxidation stability[J]. Foods, 2022, 11(4): 537.
|
| [66] |
Deng S, Shi S, Xia X. Effect of plant polyphenols on the physicochemical properties, residual nitrites, and N-nitrosamine formation in dry-fried bacon[J]. Meat Sci, 2022, 191: 108872.
|
| [67] |
Lan W, Zhao X, Wang M, et al. Effects of chitosan and apple polyphenol coating on quality and microbial composition of large yellow croaker (Pseudosciaena crocea) during ice storage[J]. J Sci Food Agric, 2022, 102(8): 3099-3106.
|
| [68] |
Cao J, Yang L, Ye B, et al. Effect of apple polyphenol and three antioxidants on the formation of polycyclic aromatic hydrocarbon in barbecued pork[J]. Polycyclic Aromatic Compounds, 2023, 43(7): 6076-6087.
|
| [69] |
Tang P, Li X, Li H, et al. Development of active film based on collagen and hydroxypropyl methylcellulose incorporating apple polyphenol for food packaging[J]. International Journal of Biological Macromolecules, 2024, 273: 132960.
|
| [70] |
Li X, Liu Y, Luo B, et al. Effect of apple polyphenols on physicochemical properties of pea starch/pulp cellulose nanofiber composite biodegradable films[J]. International Journal of Biological Macromolecules, 2024, 257: 128480.
|
| [71] |
Liu X, Chi Y, Zhang H. Improved properties of polysaccharide-based E dible films incorporated with egg white protein and apple polyphenols and application to Ql uality preservation of cashews during storage[J]. Food Science, 2021, 42(13): 174-184.
|
| [72] |
Shen S, Wang Y, Zhang Y. Preparation and rese earch of sodium algin ate/chitosan/apple polyphenol composite film[J]. China Plastics Industry, 2021, 49(11): 157-161+172.
|
| [73] |
Shi J, Wang X, Xu C. Preparation and properties of chitosan/gelat tin/apple polyphenol composite films[J]. Packaging Engineering, 2023, 44(1): 1-6.
|
| [74] |
Li X, Liu Y, Luo B, et al. Effect of apple polyphenols on physicochemical properties of pea starch/pulp cellulose nanofiber composite biodegradable films[J]. Int J Biol Macromol, 2024, 257(Pt 1): 128480.
|
| [75] |
Lin L, Peng S, Shi C, et al. Preparation and characterization of cassava starch/sodium carboxymethyl cellulose edible film incorporating apple polyphenols[J]. Int J Biol Macromol, 2022, 212: 155-164.
|
| [76] |
Gong T, Song Z, Zhang S, et al. Young apple polyphenols confer excellent physical and oxidative stabilities to soy protein emulsions for effective β-carotene encapsulation and delivery[J]. International Journal of Biological Macromolecules, 2024, 275: 133607.
|
| [77] |
Song ZC, Zhang H, Niu PF, et al. Fabrication of a novel antioxidant emulsifier through tuning the molecular interaction between soy protein isolates and young apple polyphenols[J]. Food Chemistry, 2023, 420: 136110.
|
| [78] |
Ahmad I, Khalique A, Shahid MQ, et al. Studying the influence of apple peel polyphenol extract fortification on the characteristics of probiotic yoghurt[J]. Plants (Basel), 2020, 9(1): 77.
|
| [79] |
Niu P, Wang F, Yuan K, et al. Alkaline-extracted thinned young apple polyphenols as an effective scavenger against nitrite in pickles: A comparative study with ethanol- extracted polyphenols[J]. Food Control, 2021, 130: 108387.
|
| [80] |
Gumul D, Ziobro R, Korus J, et al. Apple pomace as a source of bioactive polyphenol compounds in gluten-free breads[J]. Antioxidants (Basel), 2021, 10(5): 807.
|
| [81] |
Kruczek M, Gumul D, Korus A, et al. Phenolic compounds and antioxidant status of cookies supplemented with apple pomace[J]. Antioxidants (Basel), 2023, 12(2): 324.
|
| [82] |
Deng Y, Zhao G, Cheng K, et al. Effect of apple polyphenols on the antioxidant activity and structure of three-dimensional printed processed cheese[J]. Foods, 2023, 12(8): 1731.
|
| [83] |
Liu T, Shen H, Wang F, et al. Thinned-young apple polyphenols inhibit halitosis-related bacteria through damage to the cell membrane[J]. Front Microbiol, 2021, 12: 745100.
|
| [84] |
Wang X, Liu F, Cui Y, et al. Apple polyphenols extracts ameliorate high carbohydrate diet-induced body weight gain by regulating the gut microbiota and appetite[J]. J Agric Food Chem, 2022, 70(1): 196-210.
|
| [85] |
Han M, Zhang M, Wang X, et al. Cloudy apple juice fermented by lactobacillus prevents obesity via modulating gut microbiota and protecting intestinal tract health[J]. Nutrients, 2021, 13(3): 971.
|
| [86] |
Li D, Cui Y, Wang X, et al. Apple polyphenol extract alleviates lipid accumulation in free-fatty-acid-exposed HepG2 cells via activating autophagy mediated by SIRT1/AMPK signaling[J]. Phytother Res, 2021, 35(3): 1416-1431.
|