This study employs combined network pharmacology and molecular docking approaches to investigate the potential mechanisms by which Erigeron breviscapus polyphenols inhibit liver fibrosis. Active compounds were identified through literature mining, with targets predicted using TCMSP, PubChem, SwissTarget, and SwissADME databases. Liver fibrosis- related targets were retrieved from GeneCards, OMIM, and TTD. Following rigorous screening, 12 bioactive polyphenolic compounds and 117 corresponding targets were identified, intersecting with 8,375 liver fibrosis targets to yield 67 common targets. Protein-protein interaction analysis revealed 80 key targets (e.g., EGFR, ESR1, PTGS2). GO and KEGG analyses indicated enrichment in 352 biological terms and 50 pathways, including chemical carcinogenesis receptor activation and steroid hormone biosynthesis. Molecular docking confirmed effective binding affinity between the top four compounds (by degree value) and their respective targets. In summary, the results of this study indicate that Erigeron breviscapus can inhibit the development of liver fibrosis and related diseases through multiple components, targets, and pathways. This study provides a solid theoretical basis for the research of Erigeron breviscapus in the field of anti liver fibrosis.
The aim of this study was to enhance the applicability of genistein (GEN) and investigate genistein solid dispersion (GEN-SD). The optimal process parameters were determined as follows: anhydrous ethanol volume ratio of 4:1, ultrasonication time of 30 min, rotary evaporation temperature of 50 °C, and a drug-to-carrier mass ratio of 1:7. The results of the dissolution and solubility experiments showed that the dissolution rate and solubility of the optimized solid dispersion were significantly improved compared to pure GEN. Comprehensive characterization of the GEN-SD using X-ray diffraction, differential scanning calorimetry, scanning electron microscopy, and Fourier transform infrared spectroscopy clarified variations in crystalline form, thermal properties, and microscopic morphology. Antioxidant experiments showed that GEN- SD exhibited antioxidant activity and could effectively scavenge various free radicals. Stability studies demonstrated that GEN-SD was stable at a high temperature of 60 ℃ and a light intensity of 4500 lx.
The aim of this project is to explore the mechanism of the treatment of alcoholic liver by means of network pharmacology and molecular docking. In this study, the chemical components of the extract of Callistephus chinensis (L.) Nees. were characterized by LC-MS/MS, identifying 6 compounds by positive and negative total ion flow maps. The target of chrysanthemum was derived from SwissTargetPrediction database, and the target related to alcoholic liver was derived from GeneCards and OMIM database. Add the target to the String database to build the protein interaction platform Microbiology software was used for GO bioprocess enrichment analysis and KEGG pathway enrichment analysis, and the target pathway network was constructed. In Discovery Studio 2016 Client software verified molecular docking, China aster flavonoids compounds and the adhesion strength of the key targets. Five potential active components were screened from the flavonoid monomer compounds of the chrysanthemum Cupressus. 546 Bioprocess (BP), 75 cell composition (CC) and 185·molecular function (MF) were obtained by GO enrichment analysis. KEGG enrichment analysis for each cross target involved a pathway. Network analysis showed that it was the main active component of flavonoids in the treatment of alcoholic liver, and other related signals were related to the treatment of alcoholic liver. This study showed that the flavonoids of Callistephus chinensis (L.) Nees were involved in the treatment of alcoholic liver by regulating multi-target and multi-pathway.
In the context of a surging demand for functional foods, this study utilized Sophora japonica L. (SL) and Rosa rugosa Thunb. (RT), which are rich in polyphenols (with flavonoids as the core subclass). High-purity extracts (SLE and RTE) were obtained through ethanol reflux extraction and macroporous resin purification, and then formulated with maltodextrin and erythritol to prepare a composite solid beverage. This beverage exhibited excellent antioxidant capabilities. At a concentration of 1 mg/mL, the scavenging rates of DPPH, ABTS, and hydroxyl radicals reached 82.4%, 94.6%, and 49.2%, respectively. Network pharmacology indicated that quercetin and β-sitosterol could modulate lipid metabolism pathways. Moreover, the beverage showed potential for lipid-lowering. Its cholate adsorption capacity was 589.4 ± 2.9 mg/g at pH 7.0, and the IC50 value for pancreatic lipase inhibition was 32.55 mg/mL. However, a 60-day storage stability test revealed that the moisture content approached 5%, likely due to polyphenol-flavonoid reactivity, extending dissolution time to 30.88 s. These changes were attributed to polyphenols (with flavonoids as the core active subclass, and non-flavonoids such as phenolic acids as auxiliary), resulting in color alterations and reduced solubility. This study confirmed the dual functions of the SL- RT beverage in antioxidant and lipid-lowering aspects. Nevertheless, it also pointed out the need to optimize the formula and process to enhance stability, providing an important basis for the development of stable functional beverages.