The aim of this study was to explore the mechanism of action of sea buckthorn polyphenols in the treatment of hyperlipidemia through network pharmacology and molecular docking. The TCMSP pharmacology database was used to screen the polyphenols present in sea buckthorn, and then the SwissTargetPrediction and Uniprot databases were used to obtain the potential targets of sea buckthorn polyphenols, which were supplemented by the literature. In total, 7 polyphenols and 154 potential targets were obtained. Through GeneCards, OMIM database, 1 358 hyperlipidemia-related targets were collected. We found that there were 101 targets at the intersection of components and diseases. Through GO and KEGG enrichment analysis, 27 core targets were obtained, which were AKT1, TNF, TP53, IL-6, etc. in order of degree value. 174 pathways were obtained from KEGG enrichment analysis, including AGE-RAGE signaling pathway in diabetic complications, fluid shear stress and atherosclerosis, lipid and atherosclerosis, etc. The molecular docking of the main components to the targets was performed using OpenBabelGUI, AutoDockTools-1.5.6 software. Finally, the results were visualized using Cytoscape 3.9.1 software. The molecular docking results showed that sea buckthorn polyphenols have good binding ability with the key targets. Among them, such as quercetin and kaempferol, have good binding ability with TNF, TP53 and IL-6. For example, TNF binds to quercetin with a binding energy of -5.34 kcal/mol and to kaempferol with a binding energy of -6.22 kcal/mol; TP53 binds to kaempferol with a binding energy of -5.32 kcal/mol; IL-6 binds to quercetin with a binding energy of -5.62 kcal/mol, etc. Therefore, the network pharmacology study showed that the treatment of hyperlipidemia by sea buckthorn polyphenols can be realized by multi-component-multi-target-multi-pathway together, which provides some reference for the later study of sea buckthorn polyphenols in the treatment of hyperlipidemia.
Acknowledgments
This research was supported by 2024 Liaoning Province Graduate Education Teaching Reform Research Project (LNYJG2024251).
| [1] |
Wu L, Wang X, Jiang J, et al. Mechanism of rhubarb in the treatment of hyperlipidemia: A recent review[J]. Open Medicine, 2023, 18 (1): 20230812.
|
| [2] |
Tenenbaum A, Fisman EZ, Motro M, et al. Atherogenic dyslipidemia in metabolic syndrome and type 2 diabetes: Therapeutic options beyond statins[J]. Cardiovascular Diabetology, 2006, 5: 20.
|
| [3] |
Sun SS, Wang K, Ma K, et al. An insoluble polysaccharide from the sclerotium of Poria cocos improves hyperglycemia, hyperlipidemia and hepatic steatosis in ob/ob mice via modulation of gut microbiota[J]. Chinese Journal of Natural Medicines, 2019, 17 (1): 3-14.
|
| [4] |
Mao K, Liu H, Cao X, et al. Hawthorn or semen cassiae- alleviated high-fat diet-induced hepatic steatosis in rats via the reduction of endoplasmic reticulum stress[J]. Food & Function, 2022, 13 (23): 12170-12181.
|
| [5] |
Yao NN, Che FB, Zhang T, et al. Comparative analysis of different pretreatment on improving the effect of hot air drying of sea buckthorn with large fruits[J]. Modern Food Science & Technology, 2020, 36 (8): 211-219+22.
|
| [6] |
Dong K, Fernando WMADB, Durham R, et al. Nutritional value, health-promoting benefits and food application of sea buckthorn[J]. Food Reviews International, 2023, 39 (4): 2122-2137.
|
| [7] |
Criste A, Urcan AC, Bunea A, et al. Phytochemical composition and biological activity of berries and leaves from four romanian sea buckthorn (Hippophae Rhamnoides L.) Varieties[J]. Molecules, 2020, 25 (5): 1170.
|
| [8] |
Ning ZX, Niu GC, Zhu LB, et al. Progress of research on active ingredients, physiological functions and development and utilization of sea buckthorn[J]. Food & Machinery, 2021, 37 (11): 221-227+240.
|
| [9] |
Ren J, Ren M, Mo Z, et al. Study on anti-inflammatory mechanism of angelica pubescens based on network pharmacology and molecular docking[J]. Natural Product Communications, 2023, 18 (1): 1934578X221146616.
|
| [10] |
Hong M, Li S, Tan H Y, et al. A network- based pharmacology study of the herb-induced liver injury potential of traditional hepatoprotective Chinese herbal medicines[J]. Molecules, 2017, 22 (4): 632.
|
| [11] |
Xu X, Zhang W, Huang C, et al. A novel chemometric method for the prediction of human oral bioavailability[J]. International Journal of Molecular Sciences, 2012, 13 (6): 6964-6982.
|
| [12] |
Liu J, Li Y, Zhang Y, et al. A network pharmacology approach to explore the mechanisms of Qishen granules in heart failure[J]. Medical Science Monitor, 2019, 25: 7735-7745.
|
| [13] |
Montserrat-de la Paz S, Bermudez B, Carmen Naranjo M, et al. Pharmacological effects of niacin on acute hyperlipemia[J]. Current Medicinal Chemistry, 2016, 23 (25): 2826-2835.
|
| [14] |
Zhou J, Cao B, Ju W, et al. Effects of tongxinluo on angiogenesis in the carotid adventitia of hyperlipidemic rabbits[J]. Molecular Medicine Reports, 2016, 14 (4): 3832-3840.
|
| [15] |
Yuan L, Zhang W, Fang W, et al. Sea buckthorn polyphenols alleviate high-fat-diet-induced metabolic disorders in mice via reprograming hepatic lipid homeostasis owing to directly targeting fatty acid synthase[J]. Journal of Agricultural and Food Chemistry, 2024, 72 (15): 8632-8649.
|
| [16] |
Yang F, Suo Y, Chen D, et al. Protection against vascular endothelial dysfunction by polyphenols in sea buckthorn berries in rats with hyperlipidemia[J]. Bioscience Trends, 2016, 10 (3): 188-196.
|
| [17] |
Jiang YH, Jiang LY, Wang YC, et al. Quercetin attenuates atherosclerosis via modulating oxidized LDL- induced endothelial cellular senescence[J]. Frontiers in Pharmacology, 2020, 11: 772.
|
| [18] |
Zhang F, Feng J, Zhang J, et al. Quercetin modulates AMPK/SIRT1/NF-κB signaling to inhibit inflammatory/ oxidative stress responses in diabetic high fat diet-induced atherosclerosis in the rat carotid artery[J]. Experimental and Therapeutic Medicine, 2020, 20 (6): 280.
|
| [19] |
Li S, Hao M, Wu T, et al. Kaempferol alleviates human endothelial cell injury through circNOL12/miR-6873-3p/ FRS2 axis[J]. Biomedicine & Pharmacotherapy, 2021, 137: 111419.
|
| [20] |
Feng Z, Wang C, Yue J, et al. Kaempferol-induced GPER upregulation attenuates atherosclerosis via the PI3K/AKT/ Nrf2 pathway[J]. Pharmaceutical Biology, 2021, 59 (1): 1106-1116.
|
| [21] |
Huang S, Cheng P. Exploring the mechanism of action of Pu Shen capsule in the treatment of hyperlipidemia based on network pharmacology[J]. Journal of Nanjing University of Traditional Chinese Medicine, 2019, 35 (3): 290-296.
|
| [22] |
Zhang H, Ni Y, Wang SJ, et al. Research progress on the occurrence mechanism of atherosclerosis and intervention of traditional Chinese medicine[J]. Journal of Integrative Cardiovascular and Cerebrovascular Disease, 2019, 17 (21): 3342-3347.
|
| [23] |
An HJ, Kim JY, Gwon MG, et al. Beneficial effects of SREBP decoy oligodeoxynucleotide in an animal model of hyperlipidemia[J]. International Journal of Molecular Sciences, 2020, 21 (2): 552.
|
| [24] |
Kim CW, Oh ET, Park HJ. A strategy to prevent atherosclerosis via TNF receptor regulation[J]. Faseb Journal, 2021, 35 (3): e21391.
|
| [25] |
Chen L, He T, Han Y, et al. Pentamethyl quercetin improves adiponectin expression in differentiated 3T3- L1 cells via a mechanism that implicates PPAR-γ together with TNF-α and IL-6[J]. Molecules, 2011, 16 (7): 5754-5768.
|
| [26] |
International BR. Retracted: High inflammatory factor levels increase cardiovascular complications in diabetic patients undergoing coronary artery bypass grafting[J]. Biomed Research International, 2023, 2023: 9765041.
|
| [27] |
Xiao Y, Le W, Huang H, et al. Effect of electroacupuncture of “Feng long” (ST 40) on levels of blood lipid and macrophage TNF-alpha and IL-6 in hyperlipidemic rats[J]. Acupuncture Research, 2013, 38 (6): 459-464.
|
| [28] |
Moon SH, Huang CH, Houlihan SL, et al. p53 Represses the mevalonate pathway to mediate tumor suppression[J]. Cell, 2019, 176 (3): 564-590.
|
| [29] |
Vaughan CJ, Gotto AM. Update on statins: 2003[J]. Circulation, 2004, 110 (7): 886-892.
|
PDF
(5239KB)
