Cotton cellulose was extracted with ethanol in sub-and supercritical states dynamically. The degree of conversion was 95.4% and the extract yield was 55.2% when cotton cellulose was non-isothermally extracted with ethanol from 20°C to 400°C. From an engineering standpoint, in the temperature range from 200°C to 320°C, the rate of extract formation could adequately be described by a second-order reaction kinetics equation with the activation energy of 105.3 kJ/mol and the pre-exponential factor of 3.53×10⁷ s⁻¹. With the non-isothermal experimental technique, it was possible to determine the kinetic parameters; conversion degree and extract yield by one experiment.

Sparse-tree land is one of the typical lands and can be considered as one typical rough surface in boundary layer meteorology. Many lands can be classified into the kind surface in the view of scale and distribution feature of the roughness elements such as agroforest, scatter planted or growing trees, savanna and so on. The structure of surface boundary layer in sparse-tree land is analyzed and the parameters, friction velocityu⋆and roughness length z_o are deduced based on energy balance law and other physical hypothesis. The models agree well with data of wind tunnel experiments and field measurements.

Chemical analysis of ammonium, nitrate and total nitrogen in tree leaves and roots and anin-vivo bioassay for nitrate reductase activity (NRA) were used to monitor the seasonal variations in nitrogen assimilation among four coexisting dominant tree species, includingPinus koraiensis, Tilia amurensis, Fraxinus mandshurica andAcer mono, in a virgin mixed broad-leaved/Korean pine (Pinus koraiensis) forest. The soil study included individual horizons of L+F (0–5 cm), Ah (5–11 cm) and Aw (11–25 cm). All four species had nitrate and ammonium in their roots and leaves, and also NRA in leaves. This indicated that these coexisting species were adapted to ammonium + nitrate nutrition. A negative correlation existed between nitrate use and ammonium use. Ammonium concentration was higher than that of nitrate in tree leaves and roots, and also in soils, which indicated climax woody species had a relative preference for ammonium nutrition. There was a positive relationship between tree nitrogen nutrition use and soil nitrogen nutrient supply. Utilization of ammonium and nitrate as well as the seasonal patterns differed significantly between the species. Peaks of ammonium, nitrate, NRA and total nitrogen in one species were therefore not necessarily synchronous with peaks in other species, and which indicated a species-specific seasonal use of nitrogen. The species-specific temporal differentiation in nitrogen use might reduce the competition between co-existing species and may be an important mechanism promoting stability of virgin mixed broad-leaved//Korean pine forest.

The biomass and ratio of root-shoot ofPinus sylvestriformis seedlings at CO₂ concentration of 700 μL·L⁻¹ and 500 μL·L⁻¹ were measured using open-top chambers (OTCs) in Changbai Mountain during Jun. to Oct. in 1999. The results showed that doubling CO₂ concentration was benefit to seedling growth of the species (500 μL·L⁻¹ was better than 700 μL·L⁻¹) and the biomass production was increased in both above-ground and underground parts of seedlings. Carbon transformation to roots was evident as rising of CO₂ concentration.

Pinus sylvestriformis is an important species as an indicator of global climate changes in Changbai Mountain, China. The water use efficiency (WUE) of this species (11-year old) was studied on response to elevated CO₂ concentration at 500±100 μL·L⁻¹ by directly injecting CO₂ into the canopy under natural condition in 1998–1999. The results showed that the elevated CO₂ concentration reduced averagely stomatal opening, stomatal conductance and stomatal density to 78%, 80% and 87% respectively, as compared to normal ambient. The elevated CO₂ reduced the transpiration and enhances the water use efficiency (WUE) of plant.

Net photosynthetic rates (NPRs) of four species seedlings,Pinus koraiensis, Pinus sylvestriformis, Fraxinus mandshurica andPhellodendron amurense, were measured at different CO₂ concentrations and time respectively in Changbai Mountain during the growing season in 1999. The seedlings were cultivated in open-top chambers (OTCs), located outdoors and exposed to natural sunlight. The experimental objects were divided into four groups by tree species. CO₂ concentrations in chambers were kept at 500 μL·L⁻¹ and 700 μL·L⁻¹ and contrast chamber and contrast field were set. The results showed that the effects of elevated CO₂ on NPR of the trees strongly depended on tree species and time. NPRs ofPinus koreainsis andPinus sylvestriformis seedlings increased with the rising of CO₂ concentration, while that ofPhellodendron amurense andFraxinus mandshurica increased at some time and decreased at another time.

Using the closed chamber technique, thein situ measurements of N₂O and CH₄ fluxes was conducted in a broad-leaved Korean pine mixed forest ecosystem in Changbai Mountain, China, from June 1994 to October 1995. The relationships between fluxes (N₂O and CH₄) and some major environmental factors (temperature, soil water content and soil available nitrogen) were studied. A significant positive correlation between N₂O emission and air/soil temperature was observed, but no significant correlation was found between N₂O emission and soil water content (SWC). This result showed that temperature was an important controlling factor of N₂O flux. There was a significant correlation between CH₄ uptake and SWC, but no significant correlation was found between CH₄ uptake and temperature. This suggested SWC was an important factor controlling CH₄ uptake. The very significant negative correlation between logarithmic N₂O flux and soil nitrate concentration, significant negative correlation between CH₄ flux and soil ammonium content were also found.

Investigations of forest plant biological diversity were conducted in Liangshui Nature Reserve, Xiaoxing'an Mountains in eastern Heilongjiang Province. Six samples were selected to initiate fieldwork in spring, summer and autumn. Two samples were added to the whole experiment. Results showed that plant biological diversity in the natural reserve varied with seasons as well as plant growth forms. Generally the highest diversity values occurred in spring in the communities. Herb species had richer diversity than those of either shrub species or wood species. Species richness, diversity and evenness were quoted in the analysis.

Betula ermanni population was divided into three groups: the upper population (2 000–2 200 m), the middle population (1 700–2 000 m), and the down population (1 400–1 700 m) in Changbai Mountain. The dynamics ofBetula ermanni populations in subalpine vegetation are studied and the population life table, fecundity schedule, survival curves, age structure, and fecundity curves were established. The results showed that the, middle population is obviously, the transition from the upper population to the down population.

The photosynthesis and transpiration characteristics ofAdenophora lobophylla andA. potaninii, as well as stomatal behavior such as stomatal size, stomatal density, stomatal open and stomatal conductivity were measured at different altitudes. The relationship between the photosynthesis and transpiration characteristics and the stomatal behavior was analysed by correlation coefficient and path coefficient analysis with altitude changes. The results showed that the influences of stomatal behavior were not evident on the photosynthesis and transpiration characteristics ofA. Lobophylla, but evident on that ofA. potaninii.

Several equations were selected using nonlinear regression analysis for setting up growth and yield models of Dahurian larch (Larix gmelinii Rupr.) plantations. Data of 405 stem analysis trees were collected from 336 temporary plots throughout the Daxing'an Mountains. Results showed that the Richards equation was the best model for estimating tree height, stand mean height and stand dominant height by age; the Power equation was the fittest model for predicting tree volume by DBH and tree height, and the Logarithmic stand volume equation was good for predicting stand volume from age, mean height, basal area and other stand variables. These models can be used to construct volume tables, site index table and other forestry tables for Dahurian plantations.

According to the growth characteristics of natural Korean pine (Pinus Koraiensis) forests, 6 equations such as Chapman-Richards equation, Logistic equation, Power equation, and so on were selected to fit for the growth models for Korean pine forest. The growth models were developed based on 208 random trees and 240 dominant trees. Results show that the Chapman-Richards equation is the best model for estimating tree height by age and DBH, while the Parabola equation is fittest for predicting DBH by age or estimating age from DBH. The site index table of Korean pine forest was compiled by using the proportional method with the Chapman-Richards equation as the guide curve and validated by accuracy test.

The annual dynamic changes of soil nutrients were measured in pure larch plantation and in mixed larch plantation in the arboretum of Inner Mongolia Academy of Forestry Science, Huhehaote. The results showed that soil nutrients in pure larch plantations changed rapidly in July and August. The variation of soil nutrients is more stable in mixed larch plantation. Compared with the pure larch plantation, the content of soil nutrients in mixed larch plantation obviously increased. The soil degradation occurred in the pure larch plantation, and related to the forest age.

This paper reviewed the present status of research about tiger in China. At present, four survival subspecies are endangered in China. Tiger has decreased greatly in number, and its distribution has limited to some areas. The published papers on morphology and anatomy were relatively few, while much work has been done on physiology and biochemistry of captive tigers. The author pointed out a few further research fields. The studies of chromosomes, gene, cloning and establishment of gene bank should be mostly focusing fields. Feeding and breeding techniques must be improved and reinforced.

This paper introduced the past and current situation about distribution and population of wolf (Canis lupus) in different countries. Wolf once distributed widely in the world. Its distribution area has diminished greatly as their living conditions are destroyed, together with persecution from humans. The overall population of wolf is also declining. In some countries, wolves are endangered or even extinct. Now some countries have placed the wolf on the national list of protected animals. Alaska of the United States and Canada are the widely distributed areas of wolves in the world.

The microbial population in rotten living body ofSalix matsudana caused byTrametes suaveolens (L.) was researched. 11 bacteria species (1 species ofBacillus, 2 species ofClostridium and 8 species of non-brood-cell bacteria), 1 species ofActinomyces that belongs to Lavendulac, 8 species of fungi and 6 species ofTrichoderma were isolated from rotten trunk. The hyphae ofTrametes suaveolens mainly existed between rotten sections and discoloration sections. In over-rotten section and healthy section the fungi (Trametes suaveolens) were not isolated. The microbes that lived in the discoloration section were the most in kinds and number and they were the pioneer microbes of wood rotting. Only after they dwelled in wood and eliminated its rot-resistance, could wood-rotting fungi invade wood and caused wood-rotting.

This paper describes the basic structure and design and operation principle of the hydraulic drive and control system with two pumps and two circuits. The manipulator of the cone collecting robot designed is full driven by hydraulic, which has five freedoms. The computer and electrohydraulic proportion velocity regulating valve were installed to realize open loop serve control for reducing cost and easy application.