Oxygen solubility in perfluorocarbon (PFC) emulsions is a crucial performance metric in fields such as artificial blood, oxygen transportation and diagnostic imaging agents. However, there is a lack of simple and accurate detection methods. In this study, the perfluorooctyl bromide (PFOB) emulsion was prepared by using perfluorohexylethyl polyoxyethylene ether (FEO) as the emulsifier, and a modified enzymatic method was developed by using a more stable Orange G as the color indicator to determine the oxygen solubility in the PFOB emulsion at 298.15 K and atmospheric pressure. For 0.2 mL emulsion, the oxygen solubility measured by the modified enzymatic method was close to that measured by gas-liquid chromatography as reported in the literature. The effects of mass fractions of PFC and FEO in the emulsions on the oxygen solubility were studied based on the modified enzymatic method. The results indicate that the oxygen solubility in the emulsion increases with the increasing mass fraction of PFC, independent of the mass fraction of the emulsifier.
| [1] |
RIESS J G . Oxygen carriers (“ blood substitutes ”): raison d’etre, chemistry, and some physiology[J]. Chemical Reviews, 2001, 101(9): 2797—2920.
|
| [2] |
RIESS J G , KRAFFT M P . Fluorocarbon emulsions as in vivo oxygen delivery systems: background and chemistry[M]// LOWE K C, ed. Blood Substitutes. Oxford: Academic Press, 2006: 259—275.
|
| [3] |
VOELKER M , LAUDI S , HENKELMANN J , et al. Extracorporeal membrane oxygenation and perfluorocarbon in a therapy refractory case of acute respiratory distress syndrome[J]. The Annals of Thoracic Surgery, 2022, 113(5): e355—e358.
|
| [4] |
KRAFFT M P . Alleviating tumor hypoxia with perfluorocarbon—based oxygen carriers[J]. Current Opinion in Pharmacology, 2020, 53: 117—125.
|
| [5] |
KEIPERT P E . OxygentTM, a perfluorochemical—based oxygen therapeutic for surgical patients[M]// WINSLOW R M, ed. Blood Substitutes. Oxford: Academic Press, 2006: 312—323.
|
| [6] |
MATSUHIRA T , SAKAI H . Artificial oxygen carriers, from nanometer— to micrometer—sized particles, made of hemoglobin composites substituting for red blood cells[J]. Particuology, 2022, 64: 43—55.
|
| [7] |
JÄGERS J , WROBELN A , FERENZ K B . Perfluorocarbon—based oxygen carriers: from physics to physiology[J]. Pflugers Archiv, 2021, 473(2): 139—150.
|
| [8] |
YANG Z J , TAO D L , ZHONG W Z , et al. Perfluorocarbon loaded fluorinated covalent organic polymers with effective sonosensitization and tumor hypoxia relief enable synergistic sonodynamic—immunotherapy[J]. Biomaterials, 2022, 280:121250.
|
| [9] |
MEGURO K , WATANABE H , KATO H , et al. Study of the behavior of “ uptake and release ” of oxygen into perfluorocarbon—water emulsions[J]. Bulletin of the Chemical Society of Japan, 1983, 56(2): 386—388.
|
| [10] |
LABASQUE T , CHAUMERY C , AMINOT A , et al. Spectrophotometric Winkler determination of dissolved oxygen: re—examination of critical factors and reliability[J]. Marine Chemistry, 2004, 88(1/2): 53—60.
|
| [11] |
LIU J , LIU Q Y . Formaldehyde oxime spectrophotometry for determination of dissolved oxygen in water[J]. China Water & Wastewater, 2009, 25(2): 81—83. (in Chinese)
|
| [12] |
GHOSH A , JANIC V , SLOVITER H A . Enzymic method for measuring dissolved oxygen in nonaqueous materials[J]. Analytical Biochemistry, 1970, 38(1): 270—276.
|
| [13] |
XIAO X Z , ZHANG Y M , ZHOU L , et al. Photoluminescence and fluorescence quenching of graphene oxide: a review[J]. Nanomaterials, 2022, 12(14):2444.
|
| [14] |
FREIRE M G , DIAS A M A , COUTINHO J A P , et al. Enzymatic method for determining oxygen solubility in perfluorocarbon emulsions[J]. Fluid Phase Equilibria, 2005, 231(1): 109—113.
|
| [15] |
BANKAR S B , BULE M V , SINGHAL R S , et al. Glucose oxidase: an overview[J]. Biotechnology Advances, 2009, 27(4): 489—501.
|
| [16] |
WESSELER E P , ILTIS R , CLARK L C . The solubility of oxygen in highly fluorinated liquids[J]. Journal of Fluorine Chemistry, 1977, 9(2): 137—146.
|
| [17] |
FENG J T , YAN X Y , WANG Z Y , et al. Research progress of electron—rich materials promoting Fe3+ reduction in Fenton reaction[J]. Journal of Xi’an Jiaotong University, 2022, 56(9): 69—83. (in Chinese)
|
| [18] |
MOLLET H , GRUBENMANN A . Formulation technology: emulsions, suspensions, solid forms[M]. Weinheim: Wiley—VCH, 2007.
|
| [19] |
FREIRE M G , DIAS A M A , COELHO M A Z , et al. Aging mechanisms of perfluorocarbon emulsions using image analysis[J]. Journal of Colloid and Interface Science, 2005, 286(1): 224—232.
|
| [20] |
KHEDR A , STRIOLO A . Quantification of Ostwald ripening in emulsions via coarse—grained simulations[J]. Journal of Chemical Theory and Computation, 2019, 15(9): 5058—5068.
|
Funding
National Key R&D Program of China(2020YFB1505700)
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