Supportive angiogenic and osteogenic differentiation of mesenchymal stromal cells and endothelial cells in monolayer and co-cultures

Abstract

Interaction between developing blood vessel and bone marrow cells may improve the quality of engineered bone grafts. Florian Böhrnsen and Henning Schliephake at Germany’s University of Göttingen cultured bone marrow stem cells and cells from blood vessels together and separately, and analyzed how they differentiated. Even when grown together, cells retained their ability to differentiate into bone marrow and blood vessels, respectively. The expression of differentiation markers was increased in the co-cultures, suggesting that the two cell types reinforce each other’s differentiation. Furthermore, the presence of bone marrow cells encouraged structured growth of the blood vessels. Understanding the beneficial interactions between these two cell types is important in efforts to engineer bone grafts with a blood vessel network, and will improve our ability to successfully engineer and implant bone tissue.

Keywords

angiogenic / co-culture / differentiation / endothelial cell / mesenchymal stromal cell / osteogenic

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