Isolation and characterisation of human gingival margin-derived STRO-1/MACS⁺ and MACS⁻ cell populations

Abstract

Researchers in Germany and Egypt have identified a cell population in the gum margins with regenerative potential. A team led by Karim Fawzy El-Sayed, from the Christian Albrechts University in Kiel, Germany, and Cairo University in Egypt, magnetically sorted human gum tissue using antibodies that bind STRO-1, a surface protein that marks cells as mesenchymal stem cells. Cells expressing STRO-1 showed more molecular characteristics of stem cells, had greater proliferative capacity and could differentiate into more cell types than cells without STRO-1 on their surface. The study is the first to directly compare the two gum cell populations. STRO-1-positive cells in the gum margin could provide a renewable source of multipotent stem cells for the treatment of gingivitis and other types of periodontal disease.

Keywords

alkaline phosphatase / collagen / differentiation / gingiva / osteonectin / osteopontin / polymerase chain reaction / stem cells

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