Influence of ambient geochemical and microbiological variables on the bacterial diversity in a cold seep ecosystem in North Indian Ocean

Abstract

Cold seeps are oases for biological communities on the sea floor around hydrocarbon emission pathways. Microbial utilization of methane and other hydrocarbons yield products that fuel rich chemosynthetic communities at these sites. One such site in the cold seep ecosystem of Krishna-Godavari basin (K-G basin) along the east coast of India, discovered in Feb 2018 at a depth of 1800 m was assessed for its bacterial diversity. The seep bacterial communities were dominated by phylum Proteobacteria (57%), Firmicutes (16%) and unclassified species belonging to the family Helicobacteriaceae. The surface sediments of the seep had maximum OTUs (operational taxonomic units) (2.27 × 10³) with a Shannon alpha diversity index of 8.06. In general, environmental parameters like total organic carbon (p < 0.01), sulfate (p < 0.001), sulfide (p < 0.05) and methane (p < 0.01) were responsible for shaping the bacterial community of the cold seep ecosystem in the K-G Basin. Environmental parameters play a significant role in changing the bacterial diversity richness between different cold seep environments in the oceans.

Keywords

Sediments / Environmental variables / Proteobacteria / Cold seep ecosystem / Organic matter

CRediT authorship contribution statement

Delcy R. Nazareth: Writing – review & editing, Writing – original draft, Visualization, Validation, Software, Methodology, Formal analysis, Data curation. Maria Judith Gonsalves: Writing – review & editing, Writing – original draft, Visualization, Validation, Supervision, Data curation, Conceptualization. Nitisha Sangodkar: Writing – original draft, Visualization, Formal analysis.

Declaration of competing interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Acknowledgements

The authors are thankful to the Director, CSIR-NIO for facilities and to VNJCT for funding Ms Nazareth's fellowship. The authors are grateful to the gas hydrate group and the project leader GAP 2303 Dr. A. Mazumdar for the support and cooperation. This is NIO contribution number 7381.

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