Genomic instability evaluation in different cell line by random amplified polymorphic DNA-PCR analysis

Abstract

The cause of cancer is disorders and unstable genome. Some factors affect proper functioning of the DNA. These situations lead to cell death, cancer, inborn disorders, and overall functional decline contributing to aging. The integrity of the genome of all living organisms is constantly threatened by exogenous and endogenous DNA-damaging agents. An agent might interact directly with DNA and regulate the expression or directly by influencing DNA repair responses and so improve genomic stability. The development of widely applicable methods to monitor genomic instability gains importance nowadays. RAPD-PCR assay is a molecular method able to detect comparative DNA changes. This work is to show the applicability of the method to evaluate the ultimate changes caused in various cell-culture-derived DNA that might be a model for carcinogenesis, genomic instability and routine cell-culture work.