Frontiers in Biology >

2009 , Vol. 4 >Issue 4: 442 - 445

DOI: https://doi.org/10.1007/s11515-009-0052-3

Research articles

Identification of superoxide dismutase isoenzymes in tobacco pollen

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  • Key Laboratory for Biotechnology of the State Ethnic Affairs Commission, College of Life Science, South-Central University for Nationalities, Wuhan 430074, China;

Published date: 05 Dec 2009

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Abstract

We investigated the possible existence of superoxide dismutase (SOD; EC 1.15.1.1) isoenzymes in the pollen of Nicotiana tabacum (Petit Havana SR-1 cultivar). To detect SOD activity, crude extracts from tobacco pollen were subjected to native polyacrylamide gel electrophoresis followed by staining with nitroblue tetrazolium (NBT). The presence of six SOD isoenzymes was detected in tobacco pollen. Treatment with SOD inhibitors indicated the presence of one manganese SOD (Mn SOD), five copper-zinc SOD (Cu/Zn SOD) isoenzymes, and the absence of iron SOD (Fe SOD).

Key words： tobacco; pollen; reactive oxygen species; superoxide dismutase; fertilization

Cite this article

Jing WANG, Xuequn LIU, Guanghui YU, . Identification of superoxide dismutase isoenzymes in tobacco pollen[J]. Frontiers in Biology, 2009 , 4(4) : 442 -445 . DOI: 10.1007/s11515-009-0052-3

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