A recombinant strain of Salmonella choleraesuis C500, containing a eukaryotic expression plasmid pBO1 with the immune-dominant epitope of foot-and-mouth disease virus, was constructed. Specific immune response to this recombinant strain was evaluated by oral administration of the recombinant live bacteria pBO1/S. cho in rabbits. Results showed that T cell response and specific antibody production were elicited. This approach may present a general strategy for eliciting immune responses with DNA vaccine delivered by live bacterial vectors. The stimulated indexes of T lymphoproliferation by specific antigens of FMDV in rabbits, can reach up to 11.0 and an antibody titer of 1/32 as detected in the erum with liquid block ELISA.
Liu Mingqiu, Yan Jingran, Yan Weiyao, Zheng Zhaoxin, Niu Xiaofeng
. Immure response induced by oral DNA vaccination against FMDV delivered by attenuated Salm onella choleraesuis C500[J]. Frontiers in Biology, 2006
, 1(2)
: 110
-114
.
DOI: 10.1007/s11515-006-0020-0