Frontiers in Biology >

2006 , Vol. 1 >Issue 2: 110 - 114

DOI: https://doi.org/10.1007/s11515-006-0020-0

Immure response induced by oral DNA vaccination against FMDV delivered by attenuated Salm onella choleraesuis C500

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  • 1.State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, China; 2.Institute of Biochemistry and Cell Biology, SIBS, Chinese Academy of Sciences, Shanghai 200031, China;

Published date: 05 Jun 2006

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Abstract

A recombinant strain of Salmonella choleraesuis C500, containing a eukaryotic expression plasmid pBO1 with the immune-dominant epitope of foot-and-mouth disease virus, was constructed. Specific immune response to this recombinant strain was evaluated by oral administration of the recombinant live bacteria pBO1/S. cho in rabbits. Results showed that T cell response and specific antibody production were elicited. This approach may present a general strategy for eliciting immune responses with DNA vaccine delivered by live bacterial vectors. The stimulated indexes of T lymphoproliferation by specific antigens of FMDV in rabbits, can reach up to 11.0 and an antibody titer of 1/32 as detected in the erum with liquid block ELISA.

Cite this article

Liu Mingqiu, Yan Jingran, Yan Weiyao, Zheng Zhaoxin, Niu Xiaofeng . Immure response induced by oral DNA vaccination against FMDV delivered by attenuated Salm onella choleraesuis C500[J]. Frontiers in Biology, 2006 , 1(2) : 110 -114 . DOI: 10.1007/s11515-006-0020-0

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