Frontiers in Biology >

2006 , Vol. 1 >Issue 4: 345 - 348

DOI: https://doi.org/10.1007/s11515-006-0043-6

High cell density and high expression of recombinant human ApoA-lMilano in Escherichia coli by twice temperature-shifted induction

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  • State Key Laboratory of Bioreactor Engineering, National Research Center for Biotechnology, East China University of Science and Technology, Shanghai 200237, China;;

Published date: 05 Dec 2006

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Abstract

The effect of temperature on the formation of recombinant protein, apolipoprotein A-IMilano was investigated in the present study. The temperature of the initial growth phase was set at 30ºC, while temperature variation in induction phase was arranged in three modes. High cell-density culture of Escherichia coli and high expression of recombinant human by twice temperature-shifted induction were carried out. Experimental results showed that ApoA-IMilano reached 4.8 g/L with the final cell density of OD600, 150. It was found that twice temperature-shifted induction could successfully avoid the effect of acetic acid on cell density and the expression of the product. The present study provides a basic procedure for the production of recombinant ApoA-IMilano.

Cite this article

MA Wenfeng, GUO Meijin, DING Mansheng, CHU Ju, ZHANG Siliang . High cell density and high expression of recombinant human ApoA-lMilano in Escherichia coli by twice temperature-shifted induction[J]. Frontiers in Biology, 2006 , 1(4) : 345 -348 . DOI: 10.1007/s11515-006-0043-6

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