Frontiers in Biology >

2006 , Vol. 1 >Issue 4: 394 - 397

DOI: https://doi.org/10.1007/s11515-006-0052-5

Intra-specific relationships among Tibetan Eared-pheasants based on randomly amplified polymorphic DNA (RAPD) analysis

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  • 1.Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Beijing Normal University, Beijing 100875, China; 2.Beijing Zoo, Beijing 100044, China;

Published date: 05 Dec 2006

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Abstract

The Tibetan Eared-pheasant Crossoptilon harmani is a rare species native to China. A captive population has been established in the Beijing Zoo since 1999. In order to determine the kinship of the offsprings in 2001, randomly amplified polymorphic DNA (RAPD) was used to examine the parenthood of seven Tibetan Eared-pheasants in the Beijing Zoo. To amplify the genomic DNA of each individual, 53 arbitrary primers were selected. The results of amplifications showed that 14 primers had clear and distinct RAPD patterns. Totally, 226 amplified fragments were generated by RAPD in this study. Cluster analysis of the seven Tibetan Eared-pheasants indicated that all the four young birds had the same father (No.5 male). This study provides a practical method to determine the relationship of offsprings whose parents are unknown in birds.

Cite this article

DING Wei, ZHANG Zhengwang, CHANG Jiang, ZHANG Er, WU Xiushan, ZHANG Jinguo . Intra-specific relationships among Tibetan Eared-pheasants based on randomly amplified polymorphic DNA (RAPD) analysis[J]. Frontiers in Biology, 2006 , 1(4) : 394 -397 . DOI: 10.1007/s11515-006-0052-5

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