Frontiers in Biology >

2007 , Vol. 2 >Issue 3: 309 - 313

DOI: https://doi.org/10.1007/s11515-007-0045-z

Analysis of total proteins in pollen of Humulus scandens Lour in Wuhan Region of China by two-dimensional electrophoresis

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  • 1.Department of Bioengineering, Hainan University, Haikou 570228, China; Institute of Allergy and Inflammation, Medical Collage of Shantou University, Shantou 515031, China; 2.Institute of Allergy and Inflammation, Medical Collage of Shantou University, Shantou 515031, China;

Published date: 05 Sep 2007

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Abstract

Total proteins in the pollen of Humulus scandens Lour, one of the most popular aeroallergens in China, were analyzed by two-dimensional electrophoresis in the current study. The proteins were extracted by Trichloracetic acid (TCA) method, and then separated by isoelectric focusing as the first dimension and SDS-PAGE as the second dimension. The spots of proteins were visualized by staining with Coomassie Brilliant Blue. After analysis with software (ImageMaster 2D), 122 different proteins were detected; isoelectric point (pI), Molecular weight (MW) and relative volume of each protein in the pollen were also discovered. This is the first high-resolution, two-dimensional protein map of the pollen of Humulus scandens Lour in China. Our finding has built a solid foundation for identification, characterization, gene cloning and standardization of allergenic proteins in the pollen of Humulus scandens Lour for further studies.

Cite this article

LI Dongdong, HE Shaoheng . Analysis of total proteins in pollen of Humulus scandens Lour in Wuhan Region of China by two-dimensional electrophoresis[J]. Frontiers in Biology, 2007 , 2(3) : 309 -313 . DOI: 10.1007/s11515-007-0045-z

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