Frontiers in Biology >

2008 , Vol. 3 >Issue 2: 165 - 169

DOI: https://doi.org/10.1007/s11515-008-0015-0

Construction of a recombinant yeast strain converting xylose and glucose to ethanol

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  • 1.College of Life Science, Capital Normal University; 2.East China University of Science and Technology

Published date: 05 Jun 2008

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Abstract

Candida shehatae gene xyl1 and Pichia stipitis gene xyl2, encoding xylose reductase (XR) and xylitol dehydrogenase (XD) respectively, were amplified by PCR. The genes xyl1 and xyl2 were placed under the control of promoter GAL in vector pYES2 to construct the recombinant expression vector pYES2-P12. Subsequently the vector pYES2-P12 was transformed into S. cerevisiae YS58 by LiAc to produce the recombinant yeast YS58-12. The alcoholic ferment indicated that the recombinant yeast YS58-12 could convert xylose to ethanol with the xylose consumption rate of 81.3%.

Cite this article

TIAN Shen, ZANG Jinxin, PAN Yaping, LIU Jikai, YANG Xiushan, YUAN Zhenhong, YAN Yongjie . Construction of a recombinant yeast strain converting xylose and glucose to ethanol[J]. Frontiers in Biology, 2008 , 3(2) : 165 -169 . DOI: 10.1007/s11515-008-0015-0

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