REVIEW

Approaches in extracellular matrix engineering for determination of adhesion molecule mediated single cell function

  • Chantal E. AYRES-SANDER ,
  • Anjelica L. GONZALEZ
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  • Department of Biomedical Engineering, Yale University, New Haven, CT 06511, USA

Received date: 01 Dec 2011

Accepted date: 29 Dec 2011

Published date: 01 Feb 2013

Copyright

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg

Abstract

The native extracellular matrix (ECM) and the cells that comprise human tissues are together engaged in a complex relationship; cells alter the composition and structure of the ECM to regulate the material and biologic properties of the surrounding environment while the composition and structure of the ECM modulates cellular processes that maintain healthy tissue and repair diseased tissue. This reciprocal relationship occurs via cell adhesion molecules (CAMs) such as integrins, selectins, cadherins and IgSF adhesion molecules. To study these cell-ECM interactions, researchers use two-dimensional substrates or three-dimensional matrices composed of native proteins or bioactive peptide sequences to study single cell function. While two-dimensional substrates provide valuable information about cell-ECM interactions, three-dimensional matrices more closely mimic the native ECM; cells cultured in three-dimensional matrices have demonstrated greater cell movement and increased integrin expression when compared to cells cultured on two-dimensional substrates. In this article we review a number of cellular processes (adhesion, motility, phagocytosis, differentiation and survival) and examine the cell adhesion molecules and ECM proteins (or bioactive peptide sequences) that mediate cell functionality.

Cite this article

Chantal E. AYRES-SANDER , Anjelica L. GONZALEZ . Approaches in extracellular matrix engineering for determination of adhesion molecule mediated single cell function[J]. Frontiers in Biology, 2013 , 8(1) : 32 -49 . DOI: 10.1007/s11515-012-1199-x

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