Gene deletor: a new tool to address gene flow and food safety concerns over transgenic crop plants
Received date: 21 Nov 2011
Accepted date: 26 Dec 2011
Published date: 01 Dec 2012
Copyright
Environmental and food safety concerns over transgenic plants have hampered commercial applications of transgenic plant technology worldwide. A recently developed transgene deletion technology, named gene deletor technology, may be used to eliminate all transgenes from pollen, seeds, fruits or other organs when functions of transgenes are no longer needed or their presence may cause concerns. In this review, I will briefly describe the principle of the gene deletor technology with major supporting experimental data. I will also explain main characteristics and requirements of the gene deletor technology. Finally, I will discuss the gene deletor technology in the context of how it may be used to alleviate environmental and food safety concerns over transgenic plants in vegetatively and sexually propagated plants, to prevent volunteer transgenic plants, to protect proprietary transgenic technologies, and to allow farmers to reuse their harvested seeds for future planting.
Yi LI . Gene deletor: a new tool to address gene flow and food safety concerns over transgenic crop plants[J]. Frontiers in Biology, 0 , 7(6) : 557 -565 . DOI: 10.1007/s11515-012-1195-1
1 |
Bayley C C, Morgan M, Dale E C, Ow D W (1992). Exchange of gene activity in transgenic plants catalyzed by the Cre-lox site-specific recombination system. Plant Mol Biol, 18(2): 353-361
|
2 |
Belostotsky D A, Meagher R B (1996). A pollen-, ovule-, and early embryo-specific poly(A) binding protein from Arabidopsis complements essential functions in yeast. Plant Cell, 8(8): 1261-1275
|
3 |
Chen L J, Lee D S, Song Z P, Suh H S, Lu B R (2004). Gene flow from cultivated rice (Oryza sativa) to its weedy and wild relatives. Ann Bot (Lond), 93(1): 67-73
|
4 |
Chen Y, Rice P A (2003). New insight into site-specific recombination from Flp recombinase-DNA structures. Annu Rev Biophys Biomol Struct, 32(1): 135-159
|
5 |
Clark D, Klee H, Dandekar A (2004). Despite benefits, commercialization of transgenic horticultural crops lags. Calif Agric, 58(2): 89-98
|
6 |
Conner A J, Glare T R, Nap J P (2003). The release of genetically modified crops into the environment. Part II. Overview of ecological risk assessment. Plant J, 33(1): 19-46
|
7 |
Corneille S, Lutz K, Svab Z, Maliga P (2001). Efficient elimination of selectable marker genes from the plastid genome by the CRE-lox site-specific recombination system. Plant J, 27(2): 171-178
|
8 |
Dale E C, Ow D W (1991). Gene transfer with subsequent removal of the selection gene from the host genome. Proc Natl Acad Sci USA, 88(23): 10558-10562
|
9 |
Dale P J, Clarke B, Fontes E M G (2002). Potential for the environmental impact of transgenic crops. Nat Biotechnol, 20(6): 567-574
|
10 |
Daniell H, Kumar S, Dufourmantel N (2005a). Breakthrough in chloroplast genetic engineering of agronomically important crops. Trends Biotechnol, 23(5): 238-245
|
11 |
Daniell H, Ruiz O N, Dhingra A (2005b). Chloroplast genetic engineering to improve agronomic traits. Methods Mol Biol, 286: 111-138
|
12 |
Gardner D S, Danneberger T K, Nelson E K (2004). Lateral spread of glyphosate-resistant transgenic creeping bentgrass (Agrostis stolonifera) lines in established turfgrass swards. Weed Technol, 18(3): 773-778
|
13 |
Gilbertson L (2003). Cre-lox recombination: Cre-ative tools for plant biotechnology. Trends Biotechnol, 21(12): 550-555
|
14 |
Giovannetti M (2003). The ecological risks of transgenic plants. Riv Biol, 96(2): 207-223
|
15 |
Grindley N D, Whiteson K L, Rice P A (2006). Mechanisms of site-specific recombination. Annu Rev Biochem, 75(1): 567-605
|
16 |
Hare P D, Chua N H (2002). Excision of selectable marker genes from transgenic plants. Nat Biotechnol, 20(6): 575-580
|
17 |
Heuberger S, Ellers-Kirk C, Tabashnik B E, Carrière Y (2010). Pollen- and seed-mediated transgene flow in commercial cotton seed production fields. PLoS ONE, 5(11): e14128
|
18 |
Hoa T T C, Bong B B, Huq E, Hodges T K (2002). Cre/ lox site-specific recombination controls the excision of a transgene from the rice genome. Theor Appl Genet, 104(4): 518-525
|
19 |
Hoff T, Schnorr K M, Mundy J (2001). A recombinase-mediated transcriptional induction system in transgenic plants. Plant Mol Biol, 45(1): 41-49
|
20 |
Iamtham S, Day A (2000). Removal of antibiotic resistance genes from transgenic tobacco plastids. Nat Biotechnol, 18(11): 1172-1176
|
21 |
Jones P B C (2005). Approval for genetically engineered bentgrass creeps through agency turfs. ISB News Report, http://isb.vt.edu/articles/jan0504.htm
|
22 |
Justman M (2008). Enginerrin Agriculture (The 10th briefs on the top areas for technology innovation through 2025). (http://www.socialtechnologies.com/FileView.aspx?fileName=PressRelease03102008.pdf)
|
23 |
Kausch A, Hague J, Oliver M, Li Y, Daniell H, Mascia P, Watrud L, Stewart C N Jr (2010). Transgenic biofuel feedstocks and strategies for biocontainment. Biofuels, 1(1): 163-176
|
24 |
Klaus S M J, Huang F C, Golds T J, Koop H U (2004). Generation of marker-free plastid transformants using a transiently cointegrated selection gene. Nat Biotechnol, 22(2): 225-229
|
25 |
König A (2003). A framework for designing transgenic crops—science, safety and citizen’s concerns. Nat Biotechnol, 21(11): 1274-1279
|
26 |
Lauth M, Spreafico F, Dethleffsen K, Meyer M (2002). Stable and efficient cassette exchange under non-selectable conditions by combined use of two site-specific recombinases. Nucleic Acids Res, 30(21): 115e
|
27 |
Li R, Jia X, Mao X (2005). Ethanol-inducible gene expression system and its applications in plant functional genomics. Plant Sci, 169(3): 463-469
|
28 |
Li Y, Duan H, Smith W (2007). Gene-deletor: a new tool to address concerns over GE crops. USDA Information Systems for Biotechnology News Report, June
|
29 |
Luo H, Lyznik L A, Gidoni D, Hodges T K (2000). FLP-mediated recombination for use in hybrid plant production. Plant J, 23(3): 423-430
|
30 |
Luo K, Duan H, Zhao D, Zheng X, Deng W, Chen Y, Stewart C N Jr, McAvoy R, Jiang X, Wu Y, He A, Pei Y, Li Y (2007). ‘GM-gene-deletor’: fused loxP-FRT recognition sequences dramatically improve the efficiency of FLP or CRE recombinase on transgene excision from pollen and seed of tobacco plants. Plant Biotechnol J, 5(2): 263-274
|
31 |
Lyznik L A, Gordon-Kamm W J, Tao Y (2003). Site-specific recombination for genetic engineering in plants. Plant Cell Rep, 21(10): 925-932
|
32 |
Magaña-Gómez J A, de la Barca A M (2009). Risk assessment of genetically modified crops for nutrition and health. Nutr Rev, 67(1): 1-16
|
33 |
Mallet J, Porter P (1992). Preventing insect adaptation to insect-resistant crops: Are seed mixtures or refugia the best strategy? Proceedings B is the Royal Society B, 250, 165-169
|
34 |
Mehendale H M (2004). Genetically modified foods get bad rap. Int J Toxicol, 23(2): 79-80
|
35 |
Messeguer J (2003). Gene flow assessment in transgenic plants. Plant Cell Tissue Organ Cult, 73(3): 201-212
|
36 |
Mlynárová L, Conner A J, Nap J P (2006). Directed microspore-specific recombination of transgenic alleles to prevent pollen-mediated transmission of transgenes. Plant Biotechnol J, 4(4): 445-452
|
37 |
Moon H S, Li Y, Stewart C N Jr (2010). Keeping the genie in the bottle: transgene biocontainment by excision in pollen. Trends Biotechnol, 28(1): 3-8
|
38 |
Muller B (2006). Infringing and trespassing plants. Patented seeds at dispute in Canada's courts. Focal European Journal of Anthropology, 48: 83-98
|
39 |
Nern APfeiffer, B.D. Svoboda, K. & Rubin, G.M. (2011) Multiple new site-specific recombinases for use in manipulating animal genomes. Proceedings of the National Academy of Sciences, USA, 108, 14198-14203.
|
40 |
Odell J, Caimi P, Sauer B, Russell S (1990). Site-directed recombination in the genome of transgenic tobacco. Mol Gen Genet, 223(3): 369-378
|
41 |
Oliver M J, Quisenberry J E, Trolinder N L G, Keim D L (1998). US United States Patent Number <patent>5723765</patent>: Control of Plant Gene Expression
|
42 |
Ow D W (2001). The right chemistry for marker gene removal? Nat Biotechnol, 19(2): 115-116
|
43 |
Ow D W (2002). Recombinase-directed plant transformation for the post-genomic era. Plant Mol Biol, 48(1-2): 183-200
|
44 |
Ow D W (2007). GM maize from site-specific recombination technology, what next? Curr Opin Biotechnol, 18(2): 115-120
|
45 |
Ow D W (2011). Recombinase-mediated gene stacking as a transformation operating system. J Integr Plant Biol, 53(7): 512-519
|
46 |
Redenbaugh K, McHughen A (2004). Regulatory challenges reduce opportunities for horticultural biotechnology. Calif Agric, 58(2): 106-115
|
47 |
Reichman J R, Watrud L S, Lee E H, Burdick C A, Bollman M A, Storm M J, King G A, Mallory-Smith C (2006). Establishment of transgenic herbicide-resistant creeping bentgrass (Agrostis stolonifera L.) in nonagronomic habitats. Mol Ecol, 15(13): 4243-4255
|
48 |
Rieger M A, Lamond M, Preston C, Powles S B, Roush R T (2002). Pollen-mediated movement of herbicide resistance between commercial canola fields. Science, 296(5577): 2386-2388
|
49 |
Russell S H, Hoopes J L, Odell J T (1992). Directed excision of a transgene from the plant genome. Mol Gen Genet, 234(1): 49-59
|
50 |
Senaratna T (1992). Artificial seeds. Biotechnol Adv, 10(3): 379-392
|
51 |
Shand H (2002). Terminator no solution to gene flow. Nat Biotechnol, 20(8): 775-776
|
52 |
Srivastava V, Gidoni D (2010). Site-specific gene integration technologies for crop improvement. In Vitro Cell Dev Biol Plant, 46(3): 219-232
|
53 |
Srivastava V, Ow D W (2003). Rare instances of Cre-mediated deletion product maintained in transgenic wheat. Plant Mol Biol, 52(3): 661-668
|
54 |
Stewart C N Jr, Halfhill M D, Warwick S I (2003). Transgene introgression from genetically modified crops to their wild relatives. Nat Rev Genet, 4(10): 806-817
|
55 |
Sugita K, Kasahara T, Matsunaga E, Ebinuma H (2000). A transformation vector for the production of marker-free transgenic plants containing a single copy transgene at high frequency. Plant J, 22(5): 461-469
|
56 |
van Duyne G D (2001). A structural view of cre-loxp site-specific recombination. Annu Rev Biophys Biomol Struct, 30(1): 87-104
|
57 |
Watrud L S, Lee E H, Fairbrother A, Burdick C, Reichman J R, Bollman M, Storm M, King G, van de Water P K (2004). Evidence for landscape-level, pollen-mediated gene flow from genetically modified creeping bentgrass with CP4 EPSPS as a marker. Proceedings of the National Academy of Sciences USA, 101, 14533-14538
|
58 |
Zuo J, Niu Q W, Møller S G, Chua N H (2001). Chemical-regulated, site-specific DNA excision in transgenic plants. Nat Biotechnol, 19(2): 157-161
|
/
〈 | 〉 |