Analysis of p53 expression in partial hydatidiform mole and hydropic abortion
Received date: 15 Mar 2017
Accepted date: 31 Aug 2017
Published date: 20 Nov 2017
Copyright
BACKGROUND: Gestational trophoblastic disease (GTD) is a heterogeneous group of disorders characterized by abnormal trophoblast tissue. Molar and non-molar hydropic placental changes are the most common forms of GTD. Differential diagnosis of GTD is sometimes problematic. Recently, p53 expression was identified as a good marker for distinguishing GTD types.
AIMS:Comparison of p53 expression in partial hydatidiform mole (PHM) and hydropic abortion.
METHODS: In this prospective cross-sectional study, molar and non-molar hydropic pregnancy specimens were collected. Immunohistochemical staining, based on the Labeled Streptavidin Biotin (LSAB) technique, was carried out on multiple 4mm paraffin block sections prepared from formalin-fixed trophoblastic tissues. Polymer-based Envision was used to assess p53 tumor suppressor protein immunoreactivity. p53 expression was then compared between both groups.
RESULTS: In the study, 40 patients were included: 20 with confirmed PHM and 20 with hydropic pregnancy. p53 protein was positive in 60% of patients with PHM and 25% of patients with hydropic pregnancy. The p53 positive rate was significantly higher in patients with PHM (p = 0.027). Moreover, patients with PHM had a significantly high grade of staining (p<0.001).
CONCLUSION: Our findings indicate that immunohistochemical analysis of p53 protein can be used to distinguish PHM and hydropic pregnancy.
Key words: Partial hydatidiform mole; hydropic abortion; p53 expression
Parvin Kheradmand , Maede Goudarzi , Mina Tavakoli . Analysis of p53 expression in partial hydatidiform mole and hydropic abortion[J]. Frontiers in Biology, 2017 , 12(5) : 357 -360 . DOI: 10.1007/s11515-017-1462-2
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