RESEARCH ARTICLE

Analysis of p53 expression in partial hydatidiform mole and hydropic abortion

  • Parvin Kheradmand ,
  • Maede Goudarzi ,
  • Mina Tavakoli
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  • Ahvaz Jundishapour University of Medical Sciences, Ahvaz, Iran

Received date: 15 Mar 2017

Accepted date: 31 Aug 2017

Published date: 20 Nov 2017

Copyright

2017 Higher Education Press and Springer-Verlag Berlin Heidelberg

Abstract

BACKGROUND: Gestational trophoblastic disease (GTD) is a heterogeneous group of disorders characterized by abnormal trophoblast tissue. Molar and non-molar hydropic placental changes are the most common forms of GTD. Differential diagnosis of GTD is sometimes problematic. Recently, p53 expression was identified as a good marker for distinguishing GTD types.

AIMS:Comparison of p53 expression in partial hydatidiform mole (PHM) and hydropic abortion.

METHODS: In this prospective cross-sectional study, molar and non-molar hydropic pregnancy specimens were collected. Immunohistochemical staining, based on the Labeled Streptavidin Biotin (LSAB) technique, was carried out on multiple 4mm paraffin block sections prepared from formalin-fixed trophoblastic tissues. Polymer-based Envision was used to assess p53 tumor suppressor protein immunoreactivity. p53 expression was then compared between both groups.

RESULTS: In the study, 40 patients were included: 20 with confirmed PHM and 20 with hydropic pregnancy. p53 protein was positive in 60% of patients with PHM and 25% of patients with hydropic pregnancy. The p53 positive rate was significantly higher in patients with PHM (p = 0.027). Moreover, patients with PHM had a significantly high grade of staining (p<0.001).

CONCLUSION: Our findings indicate that immunohistochemical analysis of p53 protein can be used to distinguish PHM and hydropic pregnancy.

Cite this article

Parvin Kheradmand , Maede Goudarzi , Mina Tavakoli . Analysis of p53 expression in partial hydatidiform mole and hydropic abortion[J]. Frontiers in Biology, 2017 , 12(5) : 357 -360 . DOI: 10.1007/s11515-017-1462-2

Acknowledgments

We wish to thank all our colleagues at Imam Khomeini and Golestan Hospitals, Ahvaz, Iran.

Compliance with ethics guidelines

The authors declare that they have no conflict of interest. All procedures have been approved by the appropriate ethics committee and have therefore been performed in accordance with the ethical standards laid down by the 1964 Declaration of Helsinki and its later amendments. Informed consent was assessed prior to intervention. Details disclosing the identity of the subjects under study were omitted
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