Bioactive compounds from marine Streptomycessp. VITPSA as therapeutics

Abstract

BACKGROUND: Marine actinomycetes are efficient producers of new secondary metabolites that show different biological activities, including antibacterial, antifungal, anticancer, insecticidal, and enzyme inhibition activities.

METHODS: The morphological, physiological, and biochemical properties of the strain Streptomyces sp. VITPSA were confirmed by conventional methods. Antibacterial, anti-oxidant, anti-inflammatory, anti-diabetic, and cytotoxic activities of Streptomyces sp. VITPSA extract were determined. The media were optimized for the production of secondary metabolites. Characterization and identification of secondary metabolites were conducted by high-performance liquid chromatography, gas chromatography-mass spectroscopy, and Fourier transform infrared spectroscopy analysis.

RESULTS: The strain showed significant antibacterial, anti-oxidant, and cytotoxic activities, moderate anti-inflammatory activity, and no satisfactory anti-diabetic activity. The ethyl acetate extract of Streptomyces sp. VITPSA showed maximum antibacterial activity against two gram-positive and gram-negative bacteria at 0.5 mg/mL. The antioxidant potential of the crude extract exhibited strong reducing power activity at 0.5 mg/mL with 95.1% inhibition. The cytotoxic effect was found to be an IC₅₀ of 50 µg/mL on MCF-7 cell lines. Experimental design of optimization by one-factor analysis revealed the most favorable sucrose, yeast extract, pH (7.25), and temperature (28°C) conditions for the effective production of secondary metabolites.

CONCLUSION: This study revealed that Streptomycessp. VITPSA is an excellent source of secondary metabolites with various bioactivities.

Key words： Marine actinomycetes; bioactive compounds; pathogens; oxidants

Acknowledgments

The authors acknowledge the management of VIT University for providing all necessary facilities to carry out the study.

Compliance with ethics guidelines

S. Pooja, T. Aditi, Jemimah Naine and C. Subathra Devi declare that they have no conflict of interest. This article does not contain any studies with human or animal subjects performed by any of the authors.

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