Application of glutathione to antagonize H₂O₂-induced oxidative stress in rat tracheal epithelial cells

Abstract

With increasing industrialization, numerous air pollutants are generated. This research aimed to investigate the effects of inhalation of oxidative pollutants. H₂O₂ was used to simulate oxidative air pollutants, and glutathione, a reducing agent that is widely distributed in organisms, was used as an antagonist, to protect cells from oxidative stress. H₂O₂ was diluted using two gradients (0.05 mM, 0.20 mM, 0.80 mM, 3.20 mM and 0.05 mM, 0.10 mM, 0.15 mM, 0.20 mM) and GSH was dissolved at 20 μM. MTT, MDA, ROS, GSH, and TSLP were used as biomarkers to evaluate oxidative stress and possible resulting molecular events. A dose–response relationship was observed between H₂O₂ concentrations and the above-mentioned biomarkers. Glutathione significantly reduced levels of oxidative stress.

Key words： H₂O₂; glutathione; oxidative stress; TSLP

Compliance with ethics guidelines

The authers declare that they have no competing interets.

References

1 Al-Shami A, Spolski R, Kelly J, Keane-Myers A, Leonard W J (2005). A role for TSLP in the development of inflammation in an asthma model. J Exp Med, 202(6): 829–839

PMID

2 Crow J P (1997). Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species. Nitric Oxide, 1(2): 145–157

PMID

3 Li J, Li L, Chen H, Chang Q, Liu X, Wu Y, Wei C, Li R, Kwan J K, Yeung K L, Xi Z, Lu Z, Yang X (2014). Application of vitamin E to antagonize SWCNTs-induced exacerbation of allergic asthma. Sci Rep, 4: 4275

PMID

4 Li Y, Liu D, Ai H,Chang Q, Liu D, Xia Y, Liu S, Peng N, Xi Z, Yang X, (2010). Biological evaluation of layered double hydroxides as efficient drug vehicles. Nanotechnology, 21(10): 105101

5 Lu Z, Li C M, Qiao Y, Yan Y, Yang X (2008). Effect of inhaled formaldehyde on learning and memory of mice. Indoor Air, 18(2): 77–83

PMID

6 Massacand J C, Stettler R C, Meier R, Humphreys N E, Grencis R K, Marsland B J, Harris N L (2009). Helminth products bypass the need for TSLP in Th2 immune responses by directly modulating dendritic cell function. Proc Natl Acad Sci USA, 106(33): 13968–13973

PMID

7 Meng Z (2003). Oxidative damage of sulfur dioxide on various organs of mice: sulfur dioxide is a systemic oxidative damage agent. Inhal Toxicol, 15(2): 181–195

PMID

8 Nakamura Y, Miyata M, Ohba T, Ando T, Hatsushika K, Suenaga F, Shimokawa N, Ohnuma Y, Katoh R, Ogawa H, Nakao A (2008). Cigarette smoke extract induces thymic stromal lymphopoietin expression, leading to T(H)2-type immune responses and airway inflammation. J Allergy Clin Immunol, 122(6): 1208–1214

PMID

9 Oda D, Nguyen M P, Royack G A, Tong D C (2001). H₂O₂ oxidative damage in cultured oral epithelial cells: the effect of short-term vitamin C exposure. Anticancer Res, 21(4A): 2719–2724

PMID

10 Pandey A, Ozaki K, Baumann H, Levin S D, Puel A, Farr A G, Ziegler S F, Leonard W J, Lodish H F (2000). Cloning of a receptor subunit required for signaling by thymic stromal lymphopoietin. Nat Immunol, 1(1): 59–64

PMID

11 Sagan L A (1987). What is hormesis and why haven’t we heard about it before? Health Phys, 52(5): 521–525

PMID

12 Schlagnhaufer C D, Arteca R N (1997). Ozone‐induced oxidative stress: mechanisms of action and reaction. Physiol Plant, 100(2): 264–273

13 Shuai S, Yang S Y, Liu Z M, Yang X (2015). Oxidative damage in the kidney and brain of mice induced by different nano-materials. Frontiers in Biology, 10(1): 91–96

14 Soumelis V, Reche P A, Kanzler H, Yuan W, Edward G, Homey B, Gilliet M, Ho S, Antonenko S, Lauerma A, Smith K, Gorman D, Zurawski S, Abrams J, Menon S, McClanahan T, de Waal-Malefyt Rd R, Bazan F, Kastelein R A, Liu Y J (2002). Human epithelial cells trigger dendritic cell mediated allergic inflammation by producing TSLP. Nat Immunol, 3(7): 673–680

PMID

15 Stebbing A R D (1982). Hormesis—the stimulation of growth by low levels of inhibitors. Sci Total Environ, 22(3): 213–234

PMID

16 Wu G, Fang Y Z, Yang S, Lupton J R, Turner N D (2004). Glutathione metabolism and its implications for health. J Nutr, 134(3): 489–492

PMID