RESEARCH ARTICLE

Age-dependent peculiarities modulation of activity of aldehyde scavenger enzymes in mitochondria of rat thigh muscle during stress

  • Vadim V. Davydov , 1 ,
  • Evgenya R. Grabovetskaya 2 ,
  • Amjad Hamdallah 3
Expand
  • 1. Chair of Biochemistry State Academican I.P.Pavlov Medical University, Ryazan, 390026, Russia
  • 2. Chair of Medical Chemistry Kharkov National Medical University, Kharkov, 61022, Ukraine
  • 3. Chair of Biochemistry V.N. Karazin Kharkov National University, Kharkov, 61077, Ukraine

Received date: 31 Oct 2015

Accepted date: 10 Jan 2016

Published date: 22 Mar 2016

Copyright

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg

Abstract

The purpose of this study was a comparative investigation of activity of aldehyde scavenger enzymes in mitochondrial fraction of a thigh muscle in intact and immobilized rats of different ages. It has been shown that 12-month-old (adult) rats have high basal levels of aldehyde dehydrogenase, aldehyde reductase and glutathione transferase activity in mitochondrial fraction of thigh muscle. Aldehyde dehydrogenase activity increases during immobilization stress in adult rats. This change promote to enhance the effectiveness of utilization of carbonyl products of free radical oxidation in mitochondria of skeletal muscle of 12-month-old rats during stress. Immobilization of old and pubertal rats is accompanied by metabolic preconditions leading to accumulation of endogenous aldehydes in mitochondria, and, as a result, to the injury of muscular fibers and intensification of sarcopenia manifestations.

Cite this article

Vadim V. Davydov , Evgenya R. Grabovetskaya , Amjad Hamdallah . Age-dependent peculiarities modulation of activity of aldehyde scavenger enzymes in mitochondria of rat thigh muscle during stress[J]. Frontiers in Biology, 2016 , 11(1) : 28 -31 . DOI: 10.1007/s11515-016-1383-5

Compliances with ethics guidelines

Davydov V V, Grabovetskaya E R and Amjad Hamdallah declare that they have no conflict or interest.
All institutional and national guidelines for the care and use of laboratory animals were followed.
1
Atrac C, Madnusson T A (1978). Procedure for the isolation of noradrenaline, adrenaline, dopamine, 5-hydroxytryptamine and histamine from the same tissues samples using a single column of strongly acid exchange resin. Acta Pharmacol Toxicol (Copenh), 42: 35–57

PMID

2
Bautmans I, Van Puyvelde K, Mets T (2009). Sarcopenia and functional decline: pathophysiology, prevention and therapy. Acta Clin Belg, 64(4): 303–316

DOI PMID

3
Chen C N, Brown-Borg H M, Rakoczy S G, Thompson L V (2008). Muscle disuse: adaptation of antioxidant systems is age dependent. J Gerontol A Biol Sci Med Sci, 63(5): 461–466

PMID

4
Davydov V V, Amjad Hamdallah, Grabovetskaya E R (2014). Age-related peculiarities of change in content of free radical oxidation products in muscle during stress. Front Biol, 9(4): 283–286

DOI

5
Davydov V V, Bozhkov A I, Kulchitski O K (2012). Physiological and pathophysiological role of endogenous aldehydes. Saarbrucken: Palmarium Academic Publishing, 240 (in Russian)

6
Davydov V V, Dobaeva N M, Bozhkov A I (2004). Possible role of alteration of aldehyde’s scavenger enzymes during aging. Exp Gerontol, 39(1): 11–16

DOI PMID

7
Davydov V V, Shvets V N (2003). Age-dependent differences in the stimulation of lipid peroxidation in the heart of rats during immobilization stress. Exp Gerontol, 38(6): 693–698

DOI PMID

8
Grabovetskaya E R, Davydov V V (2009). Age peculiarities in changes activity of enzymes these take part in utilization of aldehydes in heart mitochondria during stress. Problems of Aging and Longevity, 18 (2): 166 –173

9
Hindle A G, Lawler J M, Campbell K L, Horning M (2010). Muscle aging and oxidative stress in wild-caught shrews. Comp Biochem Physiol B Biochem Mol Biol, 155(4): 427–434

DOI PMID

10
Kang C, Chung E, Diffee G, Ji L L (2013). Exercise training attenuates aging-associated mitochondrial dysfunction in rat skeletal muscle: role of PGC-1a. Exp Gerontol, 48(11): 1343–1350

DOI PMID

11
López-Torres M, Pérez-Campo R, Rojas C, Barja de Quiroga C (1992). Sensitivity to in vitro lipid peroxidation in liver and brain of aged rats. Rev Esp Fisiol, 48(3): 191–196

PMID

12
Mannervik B, Guthenberg C (1981). Glutathione transferase (human placenta). Methods Enzymol, 77: 231–235

DOI PMID

13
Marzetti E, Calvani R, Cesari M, Buford T W, Lorenzi M, Behnke B J, Leeuwenburgh C (2013). Mitochondrial dysfunction and sarcopenia of aging: from signaling pathways to clinical trials. Int J Biochem Cell Biol, 45(10): 2288–2301

DOI PMID

14
Meerson F Z (1984). Pathogenesis and prevention of stress and ischemic injures of heart. Moscow. Medicina (B Aires), 270 (in Russian)

15
Michalíková S, Balázová H, Jezová D, Kvetnanský R (1990). Changes in circulating catecholamine levels in old rats under basal conditions and during stress. Bratisl Lek Listy, 91(9): 689–693

PMID

16
Narici M V, Maffulli N (2010). Sarcopenia: characteristics, mechanisms and functional significance. Br Med Bull, 95: 139–159

DOI PMID

17
Pirozhkov S V, Panchenko L F (1988). The role of aldehyde dehydrogenases in the malonic dialdehyde metabolism in liver of rats. Biochemistry (Mosc), 53(9): 1443–1448

18
Rossi P, Marzani B, Giardina S, Negro M, Marzatico F (2008). Human skeletal muscle aging and the oxidative system: cellular events. Curr Aging Sci, 1(3): 182–191

DOI PMID

19
Srivastava S, Liu S Q, Couklin D J (2001). Involvement of aldose reductase in metabolism of atherogenic aldehyde. Chem Biol Interact, 130 –132 (1–3): 563 –571

20
Uchida K (2000). Role of reactive aldehyde in cardiovascular diseases. Free Radic Biol Med, 28(12): 1685–1696

DOI PMID

Outlines

/