Advances in quantitative proteomics

Abstract

Large-scale protein quantification has become a major proteomics application in many areas of biological and medical research. During the past years, different techniques have been developed, including gel-based such as differential in-gel electrophoresis (DIGE) and liquid chromatography-based such as isotope labeling and label-free quantification. These quantitative proteomics tools hold significant promise for biomarker discovery, diagnostic and therapeutic applications. They are also important for research in functional genomics and systems biology towards basic understanding of molecular networks and pathway interactions. In this review, we summarize current technologies in quantitative proteomics and discuss recent applications of the technologies.

Key words： two dimensional gel; liquid chromatography-mass spectrometry (LC-MS); stable isotope; quantification; application

Acknowledgements

The proteomics work in our collaborative orted by funding from the University of Florida, the National Science Foundation (MCB 0818051,CAREER 0845162onal Institute of Health (1S10RR025418-01) of the USA to S CHEN, and the National Science Foundation of China (No. 30871566) and the Natural Science Foundation of Heilongjiang Province (No. C2007-37) to H LI.

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