Oxidative damage in the kidney and brain of mice induced by different nano-materials
Received date: 18 Oct 2014
Accepted date: 26 Dec 2014
Published date: 14 Feb 2015
Copyright
With the rapid development of nanotechnology, nanomaterials have been used in numerous fields. However, these nanomaterials could also result in risk for human and environmental health. To make a comparison of the health effects of three different kinds of nanomaterials, 28 male BALB/c mice were randomly divided into four groups. Three experimental groups were exposed to different kinds of nanomaterials including graphene, graphene oxide and single wall carbon nanotubes (SWCNTs) by intraperitoneal injection while the control group received a saline injection. The exposure dose of experimental groups was 4mg/kg. After seven days, sections of mice kidney were taken, the organ coefficient of both kidney and brain was counted, and the reactive oxygen species (ROS) level, glutathione (GSH) and malondialdehyde (MDA) content was measured. Our results showed that in the experimental groups, the organ coefficient and GSH content in mice kidneys and brains decreased, whereas the ROS level and MDA content increased, when compared with the control. The graphene oxide group was statistically significant (p<0.05), while the SWCNTs group had extremely significant difference (p<0.01). Morphological changes in the kidney were also seen in the experimental groups. These results demonstrate that oxidative damage to mice kidneys and brains induced by SWCNTs and graphene oxide is more severe than graphene. The degree of damage caused by these three typical nanomaterials is different, probably due to several parameters including particle size, surface character, and shape.
Key words: graphene; graphene oxide; single wall carbon nanotubes; oxidative damage
Shuai SHANG , Shang-Yue YANG , Zhi-Min LIU , Xu YANG . Oxidative damage in the kidney and brain of mice induced by different nano-materials[J]. Frontiers in Biology, 2015 , 10(1) : 91 -96 . DOI: 10.1007/s11515-015-1345-3
1 |
Crow J P (1997). Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species. Nitric Oxide, 1(2): 145-157
|
2 |
Finkel T (2011). Signal transduction by reactive oxygen species. J Cell Biol, 194(1): 7-15
|
3 |
Gurunathan S, Han J W, Dayem A A, Eppakayala V, Kim J H (2012). Oxidative stress-mediated antibacterial activity of graphene oxide and reduced graphene oxide in Pseudomonas aeruginosa. Int J Nanomedicine, 7: 5901-5914
|
4 |
Kwon J T, Hwang S K, Jin H, Kim D S, Minai-Tehrani A, Yoon H J, Choi M, Yoon T J, Han D Y, Kang Y W, Yoon B I, Lee J K, Cho M H (2008). Body distribution of inhaled fluorescent magnetic nanoparticles in the mice. J Occup Health, 50(1): 1-6
|
5 |
Lam C W, James J T, McCluskey R, Hunter R L (2004). Pulmonary toxicity of single-wall carbon nanotubes in mice 7 and 90 days after intratracheal instillation. Toxicol Sci, 77(1): 126-134
|
6 |
Li J Q, Li L, Chen H Q, Chang Q, Liu X D, Wu Y, Wei C X, Li R, Joseph K (2014). C.Kwan, Xi ZG, Lu ZS, Yang X. Application of vitamin E to antagonize SWCNTs-induced exacerbation of allergic asthma. Scientific Reports., 4275(1): 1-10
|
7 |
Liu L H, Li T H, Zhao T K, Wang D W (2012). Research progress on graphene. Materials Review., 26(5): 37-41
|
8 |
Ma P, Zhang Z J, Jiao M, Shan S G, Wu Y, Chen J E, Yang X (2013). Oxidative damage of pesticide eypermethrin on mouse brain cells and the antioxidant role of vitamin E. China Environ Sci, 33(7): 1323-1327
|
9 |
Ni Y J. Study of combined toxicity of formaldehyde and benzene on blood system in mice. Changchun: Jilin University. 2006: 33-34.
|
10 |
Oberdörster G, Oberdörster E, Oberdörster J (2005). Nanotoxicology: an emerging discipline evolving from studies of ultrafine particles. Environ Health Perspect, 113(7): 823-839
|
11 |
Pantarotto D, Briand J P, Prato M, Bianco A (2004). Translocation of bioactive peptides across cell membranes by carbon nanotubes. Chem Commun (Camb), 7(1): 16-17
|
12 |
Raha S, Robinson B H (2000). Mitochondria, oxygen free radicals, disease and ageing. Trends Biochem Sci, 25(10): 502-508
|
13 |
Sasidharan A, Panchakarla L S, Chandran P, Menon D, Nair S, Rao C N, Koyakutty M (2011). Differential nano-bio interactions and toxicity effects of pristine versus functionalized graphene. Nanoscale, 3(6): 2461-2464
|
14 |
Sun T, Cui X, Hou Y, Zhang L, Yang M (2013). The functionalization and biocompatibility of graphene oxide. Applied Chemical Industry., 42(5): 806-808
|
15 |
Wang H L, Ke Y, Zhao M M, Wu K, Yang X (2009). Induction of oxidative stress by single wall carbon nanotubes in the liver and kidney of mice. Acta Scientiae Circumstantiae., 29(7): 1491-1495
|
16 |
Wang J W, Jiang J (2008). Advances in oxidative damage induced by single wall carbon nanotubes. Journal of Higher Correspondence Education., 21(3): 3-6
|
17 |
Zhang H, Cui H (2012). fluorescent sensors based on graphene oxide. Progress in Chemistry., 24(8): 1554-1559
|
18 |
Zhang Y, Ali S F, Dervishi E, Xu Y, Li Z, Casciano D, Biris A S (2010). Cytotoxicity effects of graphene and single-wall carbon nanotubes in neural phaeochromocytoma-derived PC12 cells. ACS Nano, 4(6): 3181-3186
|
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