Lucrative pectinase production by novel strain Pseudozyma sp. SPJ with statistical optimization techniques using agro-industrial residues
Received date: 09 Dec 2013
Accepted date: 26 May 2014
Published date: 11 Aug 2014
Copyright
Production of high titers of an alkaline, extracellular and thermo-tolerant pectinase by a newly isolated yeast Pseudozyma sp. SPJ was carried out under solid state fermentation. Citrus peel, the inexpensive agro-industrial residue used as substrate, was experienced to be unsurpassed. Response surface methodology was conducted to optimize the culture conditions for Pseudozyma sp. SPJ for hyper production of pectinase. Plackett Burman design was applied to identify the most effective culture variables. Out of nine variables studied, incubation time, moisture content and ammonium sulfate were detected as most important. A full factorial Central Composite Design was used to optimize the levels of these variables, which resulted in 17-fold increase (71.19 IU/g to 1215.66 IU/g dry substrate) in the enzyme yield. The results of analysis of variance and multiple regression analysis implies that the effect of incubation time (p<0.000) and moisture content (p<0.018) is more than ammonium sulfate. And also the interaction of moisture content with ammonium sulfate (p<0.002) is more significant.
Sampriya SHARMA , Jitender SHARMA , Rishi Pal MANDHAN . Lucrative pectinase production by novel strain Pseudozyma sp. SPJ with statistical optimization techniques using agro-industrial residues[J]. Frontiers in Biology, 2014 , 9(4) : 317 -323 . DOI: 10.1007/s11515-014-1316-0
1 |
Akhnazarova S, Kafarov V (1982). Experiment Optimization in Chemistry and chemical Engineering. Mir Publishers Moscow.
|
2 |
Bayoumi R A, Yassin H M, Swelim M A, Abdell-All E Z (2008). Production of bacterial pectinase(s) from agro-industrial wastes under solid state fermentation conditions. J ApplSci Res, 4(12): 1708–1721
|
3 |
Bogar B, Szakacs G, Pandey A, Abdulhameed S, Linden J C, Tengerdy R P (2003). Production of phytase by Mucor racemosus in solid-state fermentation. Biotechnol Prog, 19(2): 312–319
|
4 |
Box GEP, Hunter WG, Hunter JS (1978). Statistics for Experimenters, Wiley New York, 294–334
|
5 |
Box G E P, Wilson K B (1951). On the Experimental Attainment of Optimum Conditions. J R Stat Soc, B, 13: 1–45
|
6 |
Cauto S R, Sanroman M S (2005). Application of solid-state fermentation to food industry–a review. J Food Eng, 22: 211–219
|
7 |
Chen Q H, He G Q, Mokhtar A M A (2002). Optimization of medium composition for the production of elastase by Bacillus sp. EL31410 with response surface methodology. Enzyme Microb Technol, 30(5): 667–672
|
8 |
Cochran W G, Cox G M (1957). Experimental Designs. 2nd edn. Wiley New York, 346–354
|
9 |
Dhillon S S, Gill R K, Gill S S, Singh M (2004). Studies on the utilization of citrus peel for pectinase production using fungus Aspergillus niger. Int J Environ Stud, 61: 199–210
|
10 |
Garzon C G, Hours R A (1991). Citrus waste: an alternative substrate for pectinase production in solid state culture. Bioresour Technol, 39(1): 93–95
|
11 |
Gupta S, Kapoor M, Sharma K K, Nair L M, Kuhad R C (2008). Production and recovery of an alkaline exo-polygalacturonase from Bacillus subtilis RCK under solid-state fermentation using statistical approach. Bioresour Technol, 99(5): 937–945
|
12 |
Haaland PD (1989). Experimental Design in Biotechnology. Marcel Dekker New York, 1–18
|
13 |
Hoondal G S, Tiwari R P, Tewari R, Dahiya N, Beg Q K (2002). Microbial alkaline pectinases and their industrial applications: a review. Appl Microbiol Biotechnol, 59(4–5): 409–418
|
14 |
Hours R A, Voget C E, Ertola R J (1998). Some factors affecting pectinase production from apple pomace in solid state cultures. Biol Wastes, 24(2): 147–157
|
15 |
Ismail A S (1996). Utilization of orange peels for the production of multi enzyme complexes by some fungal strains. Process Biochem, 31(7): 645–650
|
16 |
Kashyap D R, Vohra P K, Chopra S, Tewari R (2001). Applications of pectinases in the commercial sector: a review. Bioresour Technol, 77(3): 215–227
|
17 |
Kuhad R C, Kapoor M, Rustagi R (2004). Enhanced production of an alkaline pectinase from Streptomyces sp. RCK-SC by whole-cell immobilization and solid state cultivation. World J Microbiol Biotechnol, 20(3): 257–263
|
18 |
Miller L G (1959). Use of dinitrosalicylic acid reagent for the determination of reducing sugars. Anal Chem, 31(3): 426–428
|
19 |
Nabi N G, Asgher M, Shah A H, Sheikh M A, Asad M J (2003). Production of pectinase by Trichoderma harzianum in solid state fermentation of citrus peel. Pak J Agri Sci, 40: 3–4
|
20 |
Narahara H, Koyama Y, Yoshida T, Pichangukura S, Ueda R, Taguchi H (1982). Growth and enzyme production in a solid-state culture of Aspergillus oryzae. J Ferment Technol, 7: 258–265
|
21 |
Pandey A, Soccol C R, Nigam P, Soccol V T (2000). Biotechnological potential of agro-industrial residues: sugar-cane bagasse. Bioresour Technol, 74(1): 69–80
|
22 |
Patil S R, Dayanand A (2006). Exploration of regional agrowastes for the production of pectinase by Aspergillusniger. Food Technol Biotechnol, 44(2): 289–292
|
23 |
Piccoli-Valle R H, Brandi I V, Silva D O, Passos F J V (2001). Pectin lyase production by Penicillium griseoroseum grown in sugar cane juice in repeated batch cultures. World J Microbiol Biotechnol, 17(5): 433–437
|
24 |
Raimbault M, Alazard D (1980). Culture method to study fungal growth in solid fermentation. Eur J Appl Microbiol Biotechnol, 9(3): 199–209
|
25 |
Reid I, Ricard M (2000). Pectinase in papermaking: solving retention problems in mechanical pulps bleached with hydrogen peroxide. Enzyme Microb Technol, 26(2–4): 115–123
|
26 |
Sharma S, Mandhan R P, Sharma J (2011a). Pseudozyma sp. SPJ: an economic and eco-friendly approach for degumming of flax fibers. World J Microbiol Biotechnol, 27(11): 2697–2701
|
27 |
Sharma S, Mandhan R P, Sharma J (2011b). Statistical optimization of culture parameters for enhanced pectinase production by a novel strain Pseudozyma sp. SPJ. Inter J Appl Eng Res, 6(5): 792–798
|
28 |
Sharma S, Mandhan R P, Sharma J (2012). Utilization of agro-industrial residues for pectinase production by the novel strain Pseudozyma sp. SPJ under solid state cultivation. Ann Microbiol, 62(1): 169–176
|
29 |
Souza M O, Roberto I C (1999). Solid-state fermentation for xylanase production by Thermoascus aurantiacus using response surface methodology. Appl Microbiol Biotechnol, 52: 768–772
|
30 |
Suryanarayan S (2003). Current industrial practice in solid state fermentations for secondary metabolite production: the Biocon India experience. Biochem Eng J, 13(2–3): 189–195
|
31 |
Tatineni R, Doddapaneni K K, Potumarthi R C, Mangamoori L N (2007). Optimization of keratinase production and enzyme activity using response surface methodology with Streptomyces sp7. Appl Biochem Biotechnol, 141(2-3): 187–201
|
32 |
Vaidya R, Vyas P, Chhatpr H S (2003). Statistical optimization of medium components for the production of chitinase by Alcaligenes xylosoxydans. Enzyme Microb Technol, 33(1): 92–96
|
33 |
Wejse P L, Ingvorsen K, Mortensen K K (2003). Xylanase production by a novel halophilic bacterium increased 20 fold by response surface methodology. Enzyme Microb Technol, 32(6): 721–727
|
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