Isolation and partial purification of fungal ligninolytic enzymes from the forest soil fungi isolated from Bhadra Wildlife Sanctuary

Abstract

Screening was done for the isolation of effective lignin degraders from the forest soil samples, by providing lignin as a carbon source through the enrichment method, which leads to the isolation of 8 effective fungal isolates among 14 isolates. Submerged fermentation was done for the production of ligninolytic enzymes with the effective microorganisms by providing Guiaicol as a carbon source. The assay of laccase, lignin peroxidise activity and specific activity was done after the incubation intervals of 2, 4, 6, 7, 8, 10 and 12 days at 27±2°C under shake culture condition. Partially purified protein content was estimated by using Lowry’s method. Pleurotus sp. and Phanerochaetae chrysosporium are more effective at the 2nd and 7th days of incubation for the production of laccase and lignin peroxidases among the effective isolates.

Key words： laccase; lignin peroxidase; extracellular enzyme; fungi; forest soil

Acknowledgements

Authors are grateful to the Kuvempu University, Jnanasahyadri, Shankaraghatta, Karnataka, India, for providing necessary facilities and support for the completion of this work. They are indebted to their family and friends for their good wishes for the success of this work.

Compliance with ethics guidelines

Shivakuamar P. BANAKAR and B. THIPPESWAMY declare that they have no conflict of interest in the publication. This article does not contain any studies with human or animal subjects performed by any of the authors.

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