Zingiber officinale extends Drosophila melanogaster life span in xenobiotic-induced oxidative stress conditions

Abstract

BACKGROUND: The possibility of dietary ginger to enhance oxidative stress resistance and to extend life span was studied on Drosophila melanogaster.

METHODS: Oxidative stress was induced by a reducing agent dithiothreitol. Experimental groups of male D. melanogaster were cultured on media containing: 1) no additive; 2) dithiothreitol, added into the nutritional mixture to the final concentration of 10 mM; 3) 25 mg of ginger powder g^–1 of the nutritional mixture; and 4) 10 mM of dithiothreitol and 25 mg of ginger powder g^–1 of the nutritional mixture. The number of alive fruit flies was inspected daily, and mean life span was determined for each experimental group.

RESULTS: The addition of dithiothreitol to D. melanogaster nutritional mixture was established to result in an increase in concentration of two markers of oxidative stress conditions (thiobarbituric acid reactive substances as products of lipid peroxidation and carbonylated proteins as products of protein oxidation) in fly tissues. It was followed by significant reduction of mean life span and maximum life span of the last 10% of flies. Plant preparation, being added simultaneously with dithiothreitol, significantly diminished the negative effects of this xenobiotic. In conditions of additional stress load induced by hydrogen peroxide or high temperature, survival of insects treated with dithiothreitol on the background of ginger powder was the highest.

CONCLUSIONS: Thus, the presented data give the evidence that ginger preparations can reduce oxidative stress outcomes and significantly increase the life expectancy of fruit flies in stress conditions.

Keywords

ginger / oxidative stress / Drosophila melanogaster

References

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Compliance with ethics guidelines

Volodymyr Padalko, Viktoriya Dzyuba, Olena Kozlova, Hanna Sheremet and Olena Protsenko declare that they have no conflict of interest. All institutional and national guidelines for the care and use of laboratory animals were followed.

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