Effect of testosterone replacement on feeding behaviors after acute and chronic stress in gonadectomized male NMRI mice
Sara Salehi Shemiran, Gholam Hossein Meftahi, Hedayat Sahraei, Negin Ghobadi
Effect of testosterone replacement on feeding behaviors after acute and chronic stress in gonadectomized male NMRI mice
BACKGROUND: We study the role of gonadectomy on the response to unavoidablestress and the role of testosterone replacement on gonadectomy inthe male Naval Medical Research Institute mice (30±5 g) werestudied. For this purpose, the hormonal and metabolic changes wereinvestigated.
METHODS: In the experimental group, the gonads were surgically removed, anda cannula was inserted into the left lateral ventricle. For acuteand chronic stress induction, animals were placed in the communicationbox for 30 min for one day and four consecutive days, respectively.The animals received different doses of intraventricular (ICV) testosterone(0.01, 0.05, 0.1 µg/mouse) 5 minutes or intraperitoneal(IP) testosterone (0.05, 0.01, 0.1 mg/kg) 30 minutes before the stressinduction.
RESULTS: The results showed that acute and chronic stress increases plasmacortisol concentration. IP testosterone injections of testosteronedid not decrease cortisol concentrations in response to acute stress,whereas ICV injections did reduce cortisol concentrations. The stressreduced anorexia time, while the administration of testosterone increasedanorexia time. In addition, acute stress reduced food intake in thegonadectomized mice. IP testosterone at 0.01 and 0.05 mg/kg increasedfood intake. Additionally, stress in gonadectomized mice reduced waterintake, while the IP injection of testosterone in chronic stress furtherreduced water intake. Also, stress reduced the animals’ brain/adrenalvolumes, while the IP and ICV injection of testosterone at 0.01 mg/kginhibited this effect.
CONCLUSION: The results showedthat the IP (0.05, 0.01, 0.1 mg/kg) and ICV (0.01, 0.05, 0.1 µg/mouse)administration of testosterone in the gonadectomized mice can modulatehormonal and metabolic changes induced by stress.
anorexia / cortisol / food intake / gonadectomy / water intake
[1] |
Adam T C, Epel E S (2007). Stress, eating and the reward system. Physiol Behav, 91(4): 449–458
CrossRef
Pubmed
Google scholar
|
[2] |
Amouei N, Sahraei H, Alibeik H , Meftahi G H , Bahari Z , Mohammadi A (2016). Intrahippocampal and peripheral effects of nicotine injection on the metabolic andbehavioral response to inescapable stress. Biosci Biotechnol Res Asia, 13(3): 1363–1371
CrossRef
Google scholar
|
[3] |
Arun S, Burawat J, Sukhorum W , Sampannang A , Uabundit N , Iamsaard S (2016). Changes of testicular phosphorylated proteins in response to restraint stressin male rats. J Zhejiang Univ Sci B, 17(1): 21–29
CrossRef
Pubmed
Google scholar
|
[4] |
Asalgoo S, Jahromi G P, Meftahi G H, Sahraei H (2015). Posttraumatic Stress Disorder (PTSD):Mechanisms and possible treatments. Neurophysiology, 47(6): 482–489
CrossRef
Google scholar
|
[5] |
Baldwin D S (2001). Depression and sexual dysfunction. Br Med Bull, 57(1): 81–99
CrossRef
Pubmed
Google scholar
|
[6] |
Chuang J C, Zigman J M (2010). Ghrelin’s roles in stress, mood, and anxiety regulation. Int J Pept, 2010: 460549
CrossRef
Pubmed
Google scholar
|
[7] |
Connan F, Lightman S L, Landau S, Wheeler M , Treasure J , Campbell I C (2007). An investigation of hypothalamic-pituitary-adrenal axis hyperactivity in anorexia nervosa:the role of CRH and AVP. J Psychiatr Res, 41(1-2): 131–143
CrossRef
Pubmed
Google scholar
|
[8] |
Cotrufo P, Monteleone P, d’Istria M , Fuschino A , Serino I , Maj M (2000). Aggressive behavioralcharacteristics and endogenous hormones in women with Bulimia nervosa. Neuropsychobiology, 42(2): 58–61
CrossRef
Pubmed
Google scholar
|
[9] |
Curtis AL, Bethea T, Valentino RJ. Sexually dimorphic responsesof the brain norepinephrine system to stress and corticotropin-releasingfactor Neuropsychopharmacol. 2006;31(3):544–54
|
[10] |
Dallman M F, Akana S F, Scribner K A, Bradbury M J, Walker C D, Strack A M, Cascio C S (1992). Stress, feedback and facilitation in the hypothalamo-pituitary-adrenalaxis. J Neuroendocrinol, 4(5): 517–526
CrossRef
Pubmed
Google scholar
|
[11] |
Dallman M F, Pecoraro N, Akana S F , La Fleur S E , Gomez F , Houshyar H , Bell M E , Bhatnagar S , Laugero K D , Manalo S (2003). Chronic stress and obesity: a new view of “comfort food”. Proc Natl Acad Sci USA, 100(20): 11696–11701
CrossRef
Pubmed
Google scholar
|
[12] |
Dalooei J R, Sahraei H, Meftahi G H , Khosravi M , Bahari Z , Hatef B , Mohammadi A , Nicaeili F , Eftekhari F , Ghamari F , Hadipour M , Kaka G (2016). Temporary amygdala inhibition reducesstress effects in female mice. J Adv Res, 7(5): 643–649
CrossRef
Pubmed
Google scholar
|
[13] |
Derijk R H, van Leeuwen N, Klok M D , Zitman F G (2008). Corticosteroid receptor-gene variants: modulators of the stress-response and implications for mental health. Eur J Pharmacol, 585(2-3): 492–501
CrossRef
Pubmed
Google scholar
|
[14] |
Ehteram B Z, Sahraei H, Meftahi G H , Khosravi M (2017). Effect of intermittent feeding on gonadal function in male and female NMRI mice during chronic stress. Braz Arch Biol Technol, 60(0): e17160607
CrossRef
Google scholar
|
[15] |
Erfani M, Sahraei H, Bahari Z , Meftahi G H , Hatef B , Mohammadi A , Hosseini S H (2017). Evaluation of the effect of time change in cognitivefunction in volunteers in Tehran. Glob J Health Sci, 9(2): 119–126
CrossRef
Google scholar
|
[16] |
Eslamizade M J , Saffarzadeh F , Mousavi S M , Meftahi G H , Hosseinmardi N , Mehdizadeh M , Janahmadi M (2015). Alterations in CA1 pyramidal neuronal intrinsic excitability mediated by Ih channel currents in a rat modelof amyloid beta pathology. Neuroscience, 305: 279–292
CrossRef
Pubmed
Google scholar
|
[17] |
Evanson N K, Herman J P (2015). Role of paraventricular nucleus glutamate signaling in regulationof HPA axis stress responses. Interdiscip Inf Sci, 21(3): 253–260
CrossRef
Pubmed
Google scholar
|
[18] |
Ghobadi N, Sahraei H, Meftahi G H , Bananej M , Salehi S (2016). Effect of estradiol replacement in ovariectomized NMRI micein responseto acute and chronic stress. J Appl Pharm Sci, 6(11): 176–184
CrossRef
Google scholar
|
[19] |
Habhab S, Sheldon J P, Loeb R C (2009). The relationship between stress, dietary restraint, and food preferences in women. Appetite, 52(2): 437–444
CrossRef
Pubmed
Google scholar
|
[20] |
Hammond J, Le Q, Goodyer C , Gelfand M , Trifiro M , LeBlanc A (2001). Testosterone mediated neuroprotection through the androgenreceptor in human primary neurons. J Neurochem,77(5):1319–1326
|
[21] |
Handa R J, Nunley K M, Lorens S A, Louie J P, McGivern R F, Bollnow M R (1994). Androgen regulation of adrenocorticotropinand corticosterone secretion in the male rat following novelty andfoot shock stressors. Physiol Behav, 55(1): 117–124
CrossRef
Pubmed
Google scholar
|
[22] |
Hardy M P, Gao H B, Dong Q, Ge R , Wang Q, Chai W R, Feng X, Sottas C (2005). Stress hormone and male reproductive function. Cell Tissue Res, 322(1): 147–153
CrossRef
Pubmed
Google scholar
|
[23] |
Hari Priya P, Reddy PS. Effect of restraint stress on lead induced male reproductive toxicity in rats. J Exp Zool A Ecol Genet Physiol 2012;317(7): 455–65
|
[24] |
Hassantash M, Sahraei H, Bahari Z , Meftahi G H , Vesali R (2017). The role of dopamine D2 receptors in the amygdala in metabolic andbehavioral responses to stress in male Swiss-Webster mice. Front Biol, 12(4): 298–310
CrossRef
Google scholar
|
[25] |
Hill J W (2010). Gene expression and the control offood intake by hypothalamic POMC/CART neurons. Open Neuroendocrinol J, 3: 21–27
Pubmed
|
[26] |
Kennedy SH, Dickens SE, Eisfeld BS , Bagby RM . Sexual dysfunction before ntidepressant therapy in majordepression. 62TJ Affect Disord 1999;56:201–208
|
[27] |
Krahn D D, Gosnell B A, Majchrzak M J (1990). The anorectic effects of CRH and restraint stress decrease with repeated exposures. Biol Psychiatry, 27(10): 1094–1102
CrossRef
Pubmed
Google scholar
|
[28] |
Lee T, Jarome T, Li S J , Kim J J , Helmstetter F J (2009). Chronic stress selectively reduces hippocampal volume in rats: a longitudinalMRI study. Neuroreport, 25: 1554–1558
CrossRef
Pubmed
Google scholar
|
[29] |
Liston C, Miller M M, Goldwater D S, Radley J J, Rocher A B, Hof P R, Morrison J H, McEwen B S (2006). Stress-induced alterations in prefrontal cortical dendritic morphology predict selectiveimpairments in perceptual attentional set-shifting. J Neurosci, 26(30): 7870–7874
CrossRef
Pubmed
Google scholar
|
[30] |
Lund T D, Hinds L R, Handa R J (2006). The androgen 5alpha-dihydrotestosteroneand its metabolite 5alpha-androstan-3beta, 17beta-diol inhibit thehypothalamo-pituitary-adrenal response to stress by acting throughestrogen receptor beta-expressing neurons in the hypothalamus. J Neurosci, 26(5): 1448–1456
CrossRef
Pubmed
Google scholar
|
[31] |
Lund T D, Munson D J, Haldy M E, Handa R J (2004). Androgen inhibits, while oestrogen enhances, restraint-inducedactivation of neuropeptide neurones in the paraventricular nucleusof the hypothalamus. J Neuroendocrinol, 16(3): 272–278
CrossRef
Pubmed
Google scholar
|
[32] |
Majzoub J A (2006). Corticotropin-releasing hormone physiology. Eur J Endocrinol, 155(suppl_1): 71–76
CrossRef
Google scholar
|
[33] |
McEwen B S (2007). Physiology and neurobiology of stressand adaptation: central role of the brain. Physiol Rev, 87(3): 873–904
CrossRef
Pubmed
Google scholar
|
[34] |
McEwen B S (2012). Brain on stress: how the social environmentgets under the skin. Proc Natl Acad SciUSA, 109(2 Suppl 2): 17180–17185
CrossRef
Pubmed
Google scholar
|
[35] |
McEwen B S (2016). In pursuit of resilience: stress,epigenetics, and brain plasticity. Ann N Y Acad Sci, 1373(1): 56–64
CrossRef
Pubmed
Google scholar
|
[36] |
McEwen B S, Milner T A (2007). Hippocampal formation: shedding light on the influence of sex andstress on the brain. Brain Res Brain ResRev, 55(2): 343–355
CrossRef
Pubmed
Google scholar
|
[37] |
McEwen B S, Nasca C, Gray J D (2016). Stress effects on neuronal structure: hippocampus, amygdala, and prefrontal cortex. Neuropsychopharmacology, 41(1): 3–23
CrossRef
Pubmed
Google scholar
|
[38] |
Meftahi G, Ghotbedin Z, Eslamizade M J , Hosseinmardi N , Janahmadi M (2015). Suppressive effects of resveratrol treatment on theintrinsic evoked excitability of CA1 pyramidal neurons. Cell J, 17(3): 532–539
Pubmed
|
[39] |
Meftahi G H, Janahmadi M, Eslamizade M J (2014). Effects of resveratrol on intrinsic neuronal properties of CA1 pyramidal neuronsin rat hippocampal slices. Physiol Pharmacol, 18: 144–155
|
[40] |
Mikolajczyk R T , El Ansari W , Maxwell A E (2009). Food consumption frequency and perceived stress and depressive symptomsamong students in three European countries. Nutr J, 8(1): 31
CrossRef
Pubmed
Google scholar
|
[41] |
Miyamoto H, Mitani F, Mukai K , Suematsu M , Ishimura Y (1999). Studies on cytogenesis in adult rat adrenal cortex: circadian and zonal variationsand their modulation by adrenocorticotropic hormone. J Biochem, 126(6): 1175–1183
CrossRef
Pubmed
Google scholar
|
[42] |
Mohammadian Z, Sahraei H, Meftahi G H , Ali-Beik H (2017). Effects of unilatral- and bilateral inhibition of rostral ventral tegmental area and central nucleus ofamygdala on morphine-induced place conditioning in male Wistar rat. Clin Exp Pharmacol Physiol, 44(3): 403–412
CrossRef
Pubmed
Google scholar
|
[43] |
Motahari A A, Sahraei H, Meftahi G H (2016). Role of nitric oxide on dopamine release and morphine-dependency. Basic Clin Neurosci, 7(4): 283–290
Pubmed
|
[44] |
Osanloo N, Sarahian N, Zardooz H , Sahraei H , Sahraei M , Sadeghi B (2015). Effects of memantine, an NMDA antagonist, on metabolic syndromes in femaleNMRI mice. Basic Clin Neurosci, 6(4): 239–252
Pubmed
|
[45] |
Papadopoulos A D , Wardlaw S L (2000). Testosterone suppresses the response of the hypothalamic-pituitary-adrenalaxis to interleukin-6. Neuroimmunomodulation, 8(1): 39–44
CrossRef
Pubmed
Google scholar
|
[46] |
Paxinos G, Franklin K B JThe mouse brain in stereotaxic coordinates. 2nd Edition, San Diego Academic Press, 2001
|
[47] |
Pourhashemi S F , Sahraei H , Meftahi G H , Hatef B , Gholipour B (2016). The effect of 20 minutes scuba diving on cognitive functionof professional scuba divers. Asian J Sports Med, 7(3): e38633
CrossRef
Pubmed
Google scholar
|
[48] |
Rai J, Pandey S N, Srivastava R K (2004). Testosterone hormone level in albino rats following restraint stress of long duration. J Anat Soc India, 53: 17–19
|
[49] |
Rubinow D R, Roca C A, Schmidt P J, Danaceau M A, Putnam K, Cizza G , Chrousos G , Nieman L (2005). Testosterone suppression of CRH-stimulated cortisol in men. Neuropsychopharmacology, 30(10): 1906–1912
CrossRef
Pubmed
Google scholar
|
[50] |
Sadeghi-Gharajehdaghi S , Sahraei H , Bahari Z , Meftahi G H , Jahromi G P , Ali-Beik H (2017). Effect of amygdaloid complex inhibition on nicotine-induced conditioned place preference in rats. J Appl Pharm Sci,7(03): 40–47
|
[51] |
Salleh M R (2008). Life event, stress and illness. Malays J Med Sci, 15(4): 9–18
Pubmed
|
[52] |
Segerstrom S C , Miller G E (2004). Psychological stress and the human immune system: a meta-analyticstudy of 30 years of inquiry. Psychol Bull, 130(4): 601–630
CrossRef
Pubmed
Google scholar
|
[53] |
Spritzer M D, Galea L A M (2007). Testosterone and dihydrotestosterone, but not estradiol,enhance survival of new hippocampal neurons in adult male rats. Dev Neurobiol, 67(10): 1321–1333
CrossRef
Pubmed
Google scholar
|
[54] |
Ulrich-Lai Y M , Figueiredo H F , Ostrander M M , Choi D C , Engeland W C , Herman J P (2006). Chronic stress induces adrenal hyperplasia and hypertrophy in a subregion-specificmanner. Am J Physiol Endocrinol Metab, 291(5): E965–E973
CrossRef
Pubmed
Google scholar
|
[55] |
Venero C, Borrell J (1999). Rapid glucocorticoid effects on excitatory amino acidlevels in the hippocampus: a microdialysis study in freely movingrats. Eur J Neurosci, 11(7): 2465–2473
CrossRef
Pubmed
Google scholar
|
[56] |
Viau V, Meaney M J (1996). The inhibitory effect of testosterone on hypothalamic-pituitary-adrenalresponses to stress is mediated by the medial preoptic area. J Neurosci, 16(5): 1866–1876
Pubmed
|
[57] |
Williamson M, Bingham B, Viau V (2005). Central organization of androgen-sensitive pathways to the hypothalamic-pituitary-adrenalaxis: implications for individual differences in responses to homeostaticthreat and predisposition to disease. Prog Neuropsychopharmacol Biol Psychiatry, 29(8): 1239–1248
CrossRef
Pubmed
Google scholar
|
[58] |
Wright A F, Goedert M, Hastie N D (1991). Familial Alzheimer’s disease. Beta amyloid resurrected. Nature, 349(6311): 653–654
CrossRef
Pubmed
Google scholar
|
[59] |
Yuen E Y, Wei J, Liu W , Zhong P , Li X, Yan Z (2012). Repeated stress suppresses glutamatereceptor expression and function in prefrontal cortex and impairsobject recognition memory. Neuron, 73: 962–977
CrossRef
Pubmed
Google scholar
|
/
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