Evaluation of miR-21 and miR-150 expression in immune thrombocytopenic purpura pathogenesis: a case-control study

Abstract

BACKGROUND: Immune thrombocytopenic purpura (ITP) is a common autoimmune disorder diagnosed with thrombocytopenia and bleeding symptoms due to production of autoantibodies (Abs) against platelets. Nowadays, microRNAs are known as novel biomarkers for diagnosis of diseases. The aim of this study was to investigate the expression levels of miR-21 and miR-150 in ITP patients and determine the role of these miRNAs in ITP pathogenesis.

MATERIALS and METHODS:Thirty newly diagnosed patients with acute ITP and 30 healthy subjects( age and sex matched) as controls were enrolled in this study. The expression level of miR-21 and miR-150 was investigated using Real-time-PCR. Comparison of demographic characteristics of the cases was done using independentt-test and chi-square test. Comparison of the expression level of miR-21 and miR-150 with the related parameters was done using independentt-test or Mann–Whitney and Kruskal–Wallis test. Spearman rho correlation coefficient was used to investigate the relationship between the expression of miR-21 and miR-150 with demographic characteristics.

RESULTS: The expression of miR-21, 150 in the patients was not different compared with the control group in general. A significant relationship between the expression of miR-21 with hemoglobin, hematocrit and red blood cell hemoglobin concentration was observed.

DISCUSSION: Expression of miR-21 and miR-150 is not associated with pathogenesis of acute ITP and can involve the synergistic role of other miRNAs. Investigation of miR-21 and miR-150 expression along with other miRNAs and cytokines can be helpful in diagnosis and pathogenesis of ITP.

Keywords

immune thrombocytopenic purpura / miR-21 / miR-150

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Acknowledgments

This paper is issued from thesis of Elahe Khodadi, MSc student of hematology and blood banking. This work was financially supported by grant TH94/1 from vice chancellor for research affairs of Ahvaz Jundishapur University of Medical Sciences.

Compliance with ethics guidelines

Authors declare that they have no conflict of interest. All the procedures performed in the studies involving human participants were in accordance with the ethical standards of local ethics committee of the Ahvaz Jundishapur University of Medical Sciences (AJUMS.REC.1393.379), as well as 1964 Helsinki declaration. Written informed consent was obtained from all patients and normal subjects.

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