Influence of ectomycorrhizal inoculation on Pinus wallichiana and Cedrus deodara seedlings under nursery conditions

Abstract

A study was undertaken to examine the extent of root colonization by four locally isolated ectomycorrhizal (ECM) fungi (Hebeloma theobrominum, Boletus dryophilus, Scleroderma citrinum and Suillus luteus) and their effects on seedling growth in Pinus wallichiana and Cedrus deodara under nursery conditions. Seedlings of the two conifers were inoculated with mycelium of ECM fungi and were grown in pots containing sterilized forest soil for six months. The percentage of ECM colonization of roots was 38%–52% in Pinus wallichiana and 33%–48% in Cedrus deodara. ECM colonization increased shoot height, needle number, shoot and root biomass and survival of inoculated seedlings. Among the four ECM fungi Hebeloma theobrominum was more effective with Pinus wallichiana and Scleroderma citrinum with Cedrus deodara in promoting seedling survival and overall growth. All the four ECM fungi used enhanced growth of inoculated seedlings and thus can be used in afforestation and regeneration programmes in degraded forests ecosystems.

Keywords

afforestation / colonization / ectomycorrhizal / mycelium / seedling survival

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Acknowledgement

The authors are highly grateful to the Department of Biotechnology, Government of India for providing financial grant for the conduct of present research.

Compliance with ethics guidelines

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2014 Higher Education Press and Springer-Verlag Berlin Heidelberg