Mass spectrometry-based phosphoproteomics in cancer research

Abstract

Phosphorylation is one of the most common post translational modifications (PTM), participating in a large number of processes to regulate cellular functions. Phosphorylation is also one of the key factors in the origin and development of cancer. The rapid development of mass spectrometric-based phosphoproteomic technologies has made it possible for high-throughput identification and quantification of phosphorylation events. In this review, we provide a general introduction and summary of the achievements made in mass spectrometry based phosphoproteomic research, including the approaches for phosphopeptide identification and quantification, as well as instrumentation and data interpretation methods. We also review some discoveries in cancer research made possible by phosphoproteomic analysis technologies.

Keywords

phosphoproteomics / mass spectrometry / cancer research / phosphopeptide enrichment / quantitative phosphoproteomics / data interpretation

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Acknowledgments

The authors wish to thank the Walther Cancer Foundation and the Notre Dame Harper Cancer Research Institute for postdoctoral funding for XY. Also, the authors appreciate many helpful discussions with the staff of the Notre Dame Mass Spectrometry and Proteomics Facility.

RIGHTS & PERMISSIONS

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg