Combination of ARDRA and RAPD genotyping techniques
in identification of  spp. genomic species

ZHANG Yong; CHEN Yuqing; TANG Yingchun; ZHANG Kouxing

doi:10.1007/s11515-008-0087-x

Front. Biol. ›› 2008, Vol. 3 ›› Issue (4) :397 -401. DOI: 10.1007/s11515-008-0087-x

Combination of ARDRA and RAPD genotyping techniques in identification of spp. genomic species

ZHANG Yong¹, CHEN Yuqing¹, TANG Yingchun², ZHANG Kouxing²

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Abstract

A total of 10 non-repetitive multi-drug-resistant Acinetobacter strains were collected. With reference to A. calcoaceticus (ATCC23055), A. baumannii (ATCC19606), A. lwoffii (ATCC17986), and A. junii (NCTC5866), DNA fingerprint technique, amplified ribosomal DNA restriction analysis (ARDRA), and random amplified polymorphism DNA (RAPD) were carried out to identify the genomic species of Acinetobacter spp. The distances between them were calculated by the unweighted pair group method with arithmetic (UPGMA). Genotypes of Acinetobacter spp. were effectively classified and an A. junii together with nine A. baumannii isolates was genomically identified. The combination of ARDRA and RAPD DNA-fingerprint technique shows high complementarity, and could be a useful tool in Acinetobacter genomic species identification.

Keywords

Acinetobacter / DNA fingerprinting / random amplified polymorphism DNA (RAPD) / cluster analysis

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ZHANG Yong, CHEN Yuqing, TANG Yingchun, ZHANG Kouxing. Combination of ARDRA and RAPD genotyping techniques in identification of spp. genomic species. Front. Biol., 2008, 3(4): 397-401 DOI:10.1007/s11515-008-0087-x