Confirmation of natural hybrids between and (Gentianaceae) based on molecular evidence

Abstract

A few individuals with intermediate morphology always appeared in the sympatric distributions of Gentiana straminea and G. siphonantha. These intermediate individuals were hypothesized to be the hybrids of

References

1. Arnold M L (1997). Natural Hybridization and Evolution. New York: Oxford University Press
2. Arnold M L, Bouck A C, Cornman R S (2003). Verne Grant and Louisiana irises:is there anything new under the sun?New Phytologist, 161(1): 143–149. doi:10.1046/j.1469-8137.2003.00856.x
3. Chen S Y, Chen S L, Xia T, Wang Y J (2005). Phylogeny of Metagentiana andrelated genera (Gentianaceae) inferred from nuclear ribosomal ITSsequences. Acta Phytotaxonomica Sinica, 43(6): 491–502 (in Chinese). doi: 10.1360/aps040136
4. Doyle J J, Doyle J L (1987). A rapidDNA isolation procedure for small quantities of fresh leaf material. Phytochemical Bulletin, 19(6): 11–15
5. Ellstrand N C, Whitkus R W, Rieseberg L H (1996). Distribution of spontaneous planthybrids. Proceedings of the National Academyof Sciences of the United States of America, 9310, 50905093. doi:10.1073/pnas.93.10.5090
6. Ferris C, King R A, Gray A J (1997). Molecular evidence for the maternalparentage in the hybrid origin of Spartinaanglica C. E. Hubbard.Molecular Ecology, 6(2): 185–187. doi:10.1046/j.1365-294X.1997.00165.x
7. Grant V (1981). Plant speciation. NewYork, USA: Columbia UniversityPress
8. Hamilton M B (1999). Four primer pairs for the amplification of chloroplastintergenic regions with intraspecific variation. Molecular Ecology, 8(3): 521–523
9. Harris S A, Ingram R (1991). ChloroplastDNA and biosystematics: the effect of intraspecific diversity andplastid transmission. Taxon, 40(3): 393–412. doi:10.2307/1223218
10. Ho T N, Liu S W (2001). A worldwidemonograph of Gentiana. Beijing: Science Press (in Chinese)
11. Koch M A, Dobes C, Mitchell-Olds T (2003). Multiple hybrid formation in naturalpopulations: concerted evolution of the internal transcribed spacerof nuclear ribosomal DNA (ITS) in North American Arabis divaricarpa (Brassicaceae). Molecular Biology and Evolution, 20(3): 338–350. doi:10.1093/molbev/msg046
12. Lexer C, Welch M, Raymond O, Rieseberg L H (2003). The origins of ecological divergence in Helianthus paradoxus (Asteraceae): Selectionon transgressive characters in a novel hybrid habitat. Evolution, 57(9): 1989–2000
13. Liu J Q, Wang Y J, Wang A L, Ohba H, Abbott R J (2006). Radiation and diversificationwithin the Ligularia-Cremanthodium-Parasenecio complex (Asteraceae) triggered by uplift of the Qinghai-TibetanPlateau. Molecular Phylogenetics and Evolution, 38(1): 31–49. doi:10.1016/j.ympev.2005.09.010
14. Mansiona G, Struwe L (2004). Genericdelimitation and phylogenetic relationships within the subtribe Chironiinae(Chironieae: Gentianaceae), with special reference to Centaurium: evidence from nrDNA and cpDNAsequences. Molecular Phylogenetics andEvolution, 32(3): 951–977. doi:10.1016/j.ympev.2004.03.016
15. Mogensen H L (1996). The hows and whys of cytoplasmic inheritance in seedplants [J]. American Journal of Botany, 83(3): 383–404. doi:10.2307/2446172
16. Pringle J S (1965). Hybridization in Gentiana (Gentianaceae): a resume of J.T. Curtis' studies. Transactions of the Wisconsin Academy of Sciences, Arts and Letters, 54(3): 283–293
17. Rauscher J T, Doyle J J, Brown A H D (2002). Internal transcribed spacer repeat-specificprimers and the analysis of hybridization in the Glycine tomentella (Leguminosae) polyploid complex. Molecular Ecology, 11(12): 2691–2702. doi:10.1046/j.1365-294X.2002.01640.x
18. Rieseberg L H (1995). The role of hybridization in evolution: old wine innew skins. American Journal of Botany, 82(7): 944–944. doi:10.2307/2445981
19. Rieseberg L H, Carney S E (1998). Planthybridization. New Phytologist, 140(4): 599–624. doi:10.1046/j.1469-8137.1998.00315.x
20. Rieseberg L H, Archer M A, Wayne R K (1999). Transgressive segregation, adaptation,and speciation. Heredity, 83(4): 363–372. doi:10.1038/sj.hdy.6886170
21. Rieseberg L H, Raymond O, Rosenthal D M, Lai Z, Livingstone K, Nakazato T, Durphy J L, Schwarzbach A E, Donovan L A, Lexer C (2003). Major Ecological Transitions in Wild Sunflowers Facilitated by Hybridization. Science, 301(5637): 1211–1216. doi:10.1126/science.1086949
22. Rieseberg L H, Wood T E, Baack E J (2006). The nature of plant species. Nature, 440(7083): 524–527. doi:10.1038/nature04402
23. Schwarzbach A E, Donovan L A, Rieseberg L H (2001). Transgressive character expressionin a hybrid sunflower species. AmericanJournal of Botany, 88(2): 270–277. doi:10.2307/2657018
24. Stebbins G L (1959). The role of hybridization in evolution. Proceedings of the American Philosophical Society, 103(4): 231–251
25. Swofford DL (2000). PAUP*. Phylogenetic Analysis Using Parsimony (3 andOther Methods). Version 4.0b10. Sunderland, MA: Sinauer Associates
26. Thompson JD, Gibson TJ, Plewniak F, Jeanmouqin F, Higgins D G (1997). The ClustalX windows interface: flexible strategies for multiple sequence alignmentaided by quality analysis tools. NucleicAcids Research, 25(24): 4876–4882. doi:10.1093/nar/25.24.4876
27. Tsukaya H, Fukuda T, Yokoyama J (2003). Hybridization and introgression between Callicarpa japonica and C. mollis (Verbenaceae) in central Japan asinferred from nuclear and chloroplast DNA sequences. Molecular Ecology, 12(11): 3003–3011. doi:10.1046/j.1365-294X.2003.01961.x
28. Wang A L, Yang M H, Liu J Q (2005). Molecular Phylogeny, recent radiationand evolution of gross morphology of the Rhubarb genus Rheum (Polygonaceae) inferred from chloroplastDNA trnL-F sequences. Annals of Botany, 96(3): 489–498. doi:10.1093/aob/mci201
29. Wang Y J, Li X J, Hao G, Liu J Q (2004). Molecular phylogeny and biogeography of Androsace (Primulaceae) and the convergent evolution of cushion morphology. Acta Phytotaxonomica Sinica, 42(6): 481–499 (in Chinese)
30. Wendel J F, Schnabel A, Seelanan T (1995). Bidirectional interlocus concertedevolution following allopolyploid speciation in cotton (Gossypium). Proceedingsof the National Academy of Sciences of the United States of America, 92(1): 280–284. doi:10.1073/pnas.92.1.280
31. White T J, Bruns T, Lee S, Taylor J (1990). Amplification and direct sequencing of fungal ribosomal RNA genesfor phylogenetics. In: Innis M, Gelfand D, SninskyJ, White T, eds. PCR Protocols: A Guide to Methods and Application.San Diego: Academic Press, 315–322
32. Yuan C C, Li P X, Wang Y F, Shi S H (2004). The Confirmation of Putative Natural Hybrid Species Meconopsis × cookei G. Taylor (Papaveraceae) Based on Nuclear RibosomalDNA ITS Region Sequence. Acta GeneticaSinica, 31(9): 901–907 (in Chinese)
33. Zhang Q, Yang R, Wang Q, Liu J Q (2005). Phylogeography of Juniperus przewalskii (Cupressaceae) inferred from the chloroplast DNA trnT-trnF sequence variation. Acta PhytotaxonomicaSinica, 43(6): 503–512 (in Chinese). doi: 10.1360/aps040148