Simulation, construction and characterization of a multi-functional thrombolytic agent with anti-thrombosis activity

Abstract

Prourokinase (scu-PA), a thrombolytic agent, was inserted between Gly118 and Ile119 with foreign anti-thrombosis functional motif (Lys-Gly-Asp-Trp-motif) to construct a multi-functional chimeric molecule. The molecular model of a chimera was simulated and predicted. The recombinant chimeric protein was expressed by the baculovirus-insect cell expression system and purified by affinity chromatography. The physico-chemical characteristics of the chimeric molecule were assayed. The thrombolytic activity was determined to be 90000 IU/mg of fibrinolytic special activity by the fibrin-plate method. The anti-thrombosis activities were also assayed with IC₅₀ of 9.6 ?M by an inhibition test of ADP-induced platelet aggregation.

Keywords

anti-thrombosis / thrombolytic agent / insect cell / baculovirus / prourokinase (scu-PA) / foreign functional motif