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Genetic variation of the genus by ISSR markers
- ZHANG Li1, ZHOU Yonghong1, DING Chunbang1, YANG Ruiwu1, LIU Shigui2
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1.Triticeae Research Institute, Sichuan Agricultural University; 2.National Laboratory of Grassland Biological Control, Sichuan University
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Published |
05 Jun 2008 |
Issue Date |
05 Jun 2008 |
We investigated the genetic variation within 32 accessions distributed to 14 species and one variety by using ISSR (inter-simple sequence repeat) markers. The results showed that genetic variation was relatively higher among the accessions. A total of 593 bands were amplified by 12 ISSR primers, of which 535 bands (90.2%) were polymorphic. Eleven to 80 polymorphic bands were amplified from each prime, with an average of 44.6 bands. The interspecies GS (genetic similarity) value ranged from 0.430 to 0.866, and the average was 0.620. Cluster analysis showed that all accessions could be classified into 4 groups by ISSR markers. The different accessions in a species were clustered together, but they had genetic variation in molecular levels. There was obvious interspecies genetic variation. Species with similar morphological characteristics and from the same areas or neighboring geographical regions were clustered together and had close relationships. ISSR markers are useful in analyzing interspecies variation in Kengyilia.
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References
1. Cai L B Zhi L 1999 A taxonomicalstudy on the genus Kengyilia Yenet J. L. Yang.Acta Phytotaxonomica Sinica37(5)451467 (in Chinese)
2. Du J K Yao Y Y Ni Z F Feng H R Sun Q X 2002 Genetic diversity revealedby ISSR molecular marker in common wheat spelt compactum and progenyof recurrent selectionActa Genetica Sinica29(5)445452 (in Chinese)
3. Fang D Q Roose M L 1997 Identificationof closely related citrus cultivars with inter-simple sequence repeatmarkersTheor Appl Genet95408417. doi:10.1007/s001220050577
4. Fernandez M E Figueiras A M Benito C 2002 The use of ISSR and RAPD markers for detectingDNA polymorphism, genotype identification and genetic diversity amongbarley cultivars with known originTheorAppl Genet104845851. doi:10.1007/s00122‐001‐0848‐2
5. Gilbert J E Lewis R V Wilkinson M J Caligari P D S 1999 Developing an appropriate strategy to assess genetic variabilityin plant germplasm collectionsTheor ApplGenet9811251131. doi:10.1007/s001220051176
6. Guo B Z 1987 Flora of ChinaBeijing: SciencePress9(3)51104 (in Chinese)
7. Jensen K B 1990 Cytology and taxonomy of Elymuskengii, E. grandiglumis, E. alatavicus, and E. batalini (Poaceae; Triticeae).Genome33668673
8. Jing R C He Y Q Huang Q Y Zhu Y G 2000 Analysis of the fertility restorer gene in the wild-abortive (WA)type cytoplasmic male sterility (CMS) system with the ISSR and SSLPmarkersScientia Agricultura Sinica33(2)19 (in Chinese)
9. Jonsson B O Jonsdottir I S Cronberg N 1996 Clonal diversity and allozyme variationin population of the arctic sedge Carex bigelowii (Cyperacea)J Ecology84449459. doi:10.2307/2261206
10. Keng Y L 1959 Flora Illustalis Plantarum Primarum Sinicarum (Gramineae)BeijingSciencePress342409
11. Kojima T Nagaoka T Noda K Ogihara Y 1998 Genetic linkage map of ISSR and RAPD markers in Eikorn wheat in relationto that of RFLP markersTheor Appl Genet963745. doi:10.1007/s001220050706
12. Li J B Mou T M Fang X J 2002 Identification and genetic analysis for12 elite PGMS and TGMS rices based on ISSR markersChinese Agricultural Science Bulletin18(1)69 (in Chinese)
13. Nagaoka T Ogihara Y 1997 Applicabilityof inter-simple sequence repeat polymorphisms in wheat for use DNAas markers in comparison to RFLP and RAPD markersTheor Appl Genet94597602. doi:10.1007/s001220050456
14. Nei M Li W H 1979 Mathematicalmodel for studying genetic variation in terms of restriction endonucleasesProc Natl Acad Sci USA7652695273. doi:10.1073/pnas.76.10.5269
15. Qian W Ge S Hong DY 2000 Assessment of genetic variation of Oryza granulata detected by RAPDs and ISSRsActa Botanica Sinica42(7)741750 (in Chinese)
16. Rohlf F J 1993 NTSYS-pc Numerical Taxonomy and Multivariate Analysis System.Version 1.80 [M/CD]New YorkExeter Software, Setauket
17. Sharp P J Kresis M Shewry P R Gale M D 1988 Location of β–amylasesequences in wheat and its relativesTheorAppl Genet75286290. doi:10.1007/BF00303966
18. Yang J L Yen C Baum B R 1992 Kengyilia: synopsis and key to speciesHereditas1162528. doi:10.1111/j.1601‐5223.1992.tb00200.x
19. Yang W P Oliveira A C Godwin I Scheritz K Bennetzen J L 1996 Comparisonof DNA marker technologies in characterizing plant genome diversity:variability in Chinese sorghumsCrop Sci3616691676
20. Yen C Yang J L 1990 Kengyilia gobicola, a new taxon from westChinaCan J Bot6818941897
21. Zhang L Zhou Y H Zheng Y L Zhang Y 2003a Study on karyotypes and evolution of 6 species in KengyiliaJournalof Sichuan University (Natural Science Edition)40(2)361366 (in Chinese)
22. Zhang L Zheng Y L Wei Y M Liu S G Zhou Y H 2003b Genetic variation in Kengyilia populations based on randomly amplifiedpolymorphic DNA (RAPD) analysisJournalof Beijing Forestry University25(3)1116 (in Chinese)
23. Zhang L Zhou Y H Wei Y M Zheng Y L Liu S G 2003c Relationships among Kengyilia species based on random amplifiedmicrosatellite polymorphism (RAMP)HighTechnology Letters13(4)2833 (in Chinese)
24. Zhang X Q Yen C Yang J L Yen Y 1998 Cytogenetic analyses of Kengyilia laxifloraPlant Syst Evol2127986. doi:10.1007/BF00985222
25. Zhou Y H 1994 Study on karyotypes of 5 species of KengyiliaGuihaia14(2)163169 (in Chinese)
26. Zhou Y H Zheng Y L Yang J L Yen C Jia J Z 2000 Relationship among Kengyilia species assessed by RAPD markerActa Phytotaxonomica Sinica38(6)515521 (in Chinese)
27. Zietkiewicz E Rafalski A Labuda D 1994 Genome fingerprinting by simple sequencerepeat (SSR) – anchored polymerase chain reaction amplificationGenomics20176183. doi:10.1006/geno.1994.1151